In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 6 ( 2022-6-24), p. e0270610-
Abstract:
Magnesium (Mg) is an essential macronutrient for plant growth and development. Physiological and transcriptome analyses were conducted to elucidate the adaptive mechanisms to long-term Mg deficiency (MD) in banana seedlings at the 6-leaf stage. Banana seedlings were irrigated with a Mg-free nutrient solution for 42 days, and a mock control was treated with an optimum Mg supply. Leaf edge chlorosis was observed on the 9 th leaf, which gradually turned yellow from the edge to the interior region. Accordingly, the total chlorophyll content was reduced by 47.1%, 47.4%, and 53.8% in the interior, center and edge regions, respectively, and the net photosynthetic rate was significantly decreased in the 9 th leaf. Transcriptome analysis revealed that MD induced 9,314, 7,425 and 5,716 differentially expressed genes (DEGs) in the interior, center and edge regions, respectively. Of these, the chlorophyll metabolism pathway was preferentially enriched according to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The expression levels of the five candidate genes in leaves were consistent with what is expected during chlorophyll metabolism. Our results suggest that changes in the expression of genes related to chlorophyll synthesis and decomposition result in the yellowing of banana seedling leaves, and these results are helpful for understanding the banana response mechanism to long-term MD.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0270610
DOI:
10.1371/journal.pone.0270610.g001
DOI:
10.1371/journal.pone.0270610.g002
DOI:
10.1371/journal.pone.0270610.g003
DOI:
10.1371/journal.pone.0270610.g004
DOI:
10.1371/journal.pone.0270610.g005
DOI:
10.1371/journal.pone.0270610.g006
DOI:
10.1371/journal.pone.0270610.g007
DOI:
10.1371/journal.pone.0270610.t001
DOI:
10.1371/journal.pone.0270610.s001
DOI:
10.1371/journal.pone.0270610.s002
DOI:
10.1371/journal.pone.0270610.s003
DOI:
10.1371/journal.pone.0270610.s004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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