In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 10 ( 2022-10-20), p. e0275341-
Abstract:
Early chronic pancreatitis (ECP) has been reported to advance into chronic pancreatitis, it may be critical to differentiate the pathophysiology of ECP and functional dyspepsia (FD) in patients with pancreatic enzyme abnormalities (FD-P). This study aimed to clarify differences in the pathophysiology of ECP and FD-P and to determine whether duodenal inflammatory responses in the two diseases were associated with protease-activated receptor (PAR) 2, as the trypsin receptor. Methods Eighty patients who presented with FD-P and ECP were enrolled. In duodenal specimens, PAR2 mRNA levels were determined using real-time PCR. Using immunostaining, CD68-, GLP-1-, PRG2-, and CCR2-positive cells, tight junction proteins, and PAR 2 were evaluated. Results There were no significant differences in clinical symptoms and gastric motility between ECP and FD-P patients. The CD68-positive cells infiltrations and occludin expression levels in the duodenal mucosa of patients with FD-P were significantly (p 〈 0.001 and p = 0.048, respectively) lower than those in patients with ECP. Although serum trypsin levels in ECP and FD-P patents were significantly (p 〈 0.05 and p 〈 0.001, respectively) associated with duodenal eosinophils counts, elevated trypsin levels were not significantly associated with degranulated eosinophils, occludin, claudin-1 and ZO-1 expression levels in the duodenum of either group. PAR2 mRNA levels were increased in the duodenum of patients with ECP and FD-P. PAR2 was localized in the epithelial cells of the duodenal mucosa and the surface of degranulated eosinophils in ECP and FD-P patients. Conclusions Elevated trypsin levels might be partly associated with duodenal inflammatory responses through PAR2-related degranulated eosinophils and the reduction of occludin in patients with ECP and FD-P.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0275341
DOI:
10.1371/journal.pone.0275341.g001
DOI:
10.1371/journal.pone.0275341.g002
DOI:
10.1371/journal.pone.0275341.g003
DOI:
10.1371/journal.pone.0275341.g004
DOI:
10.1371/journal.pone.0275341.g005
DOI:
10.1371/journal.pone.0275341.g006
DOI:
10.1371/journal.pone.0275341.g007
DOI:
10.1371/journal.pone.0275341.g008
DOI:
10.1371/journal.pone.0275341.g009
DOI:
10.1371/journal.pone.0275341.t001
DOI:
10.1371/journal.pone.0275341.s001
DOI:
10.1371/journal.pone.0275341.s002
DOI:
10.1371/journal.pone.0275341.s003
DOI:
10.1371/journal.pone.0275341.r001
DOI:
10.1371/journal.pone.0275341.r002
DOI:
10.1371/journal.pone.0275341.r003
DOI:
10.1371/journal.pone.0275341.r004
DOI:
10.1371/journal.pone.0275341.r005
DOI:
10.1371/journal.pone.0275341.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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