In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 19, No. 5 ( 2023-5-5), p. e1011374-
Abstract:
Aedes albopictus shows a rapid global expansion and dramatic vectorial capacity for various arboviruses, thus posing a severe threat to global health. Although many noncoding RNAs have been confirmed to play functional roles in various biological processes in Ae . albopictus , the roles of circRNA remain a mystery. In the present study, we first performed high-throughput circRNA sequencing in Ae . albopictus . Then, we identified a cysteine desulfurase ( CsdA ) superfamily gene-originated circRNA, named aal-circRNA-407, which was the third most abundant circRNA in adult females and displayed a fat body highly expressed manifestation and blood feeding-dependent onset. SiRNA-mediated knockdown of circRNA-407 resulted in a decrease in the number of developing follicles and a reduction in follicle size post blood meal. Furthermore, we demonstrated that circRNA-407 can act as a sponge of aal-miR-9a-5p to promote the expression of its target gene Foxl and eventually regulate ovarian development. Our study is the first to report a functional circRNA in mosquitoes, expanding our current understanding of important biological roles in mosquitoes and providing an alternative genetic strategy for mosquito control.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1011374
DOI:
10.1371/journal.ppat.1011374.g001
DOI:
10.1371/journal.ppat.1011374.g002
DOI:
10.1371/journal.ppat.1011374.g003
DOI:
10.1371/journal.ppat.1011374.g004
DOI:
10.1371/journal.ppat.1011374.g005
DOI:
10.1371/journal.ppat.1011374.g006
DOI:
10.1371/journal.ppat.1011374.g007
DOI:
10.1371/journal.ppat.1011374.g008
DOI:
10.1371/journal.ppat.1011374.g009
DOI:
10.1371/journal.ppat.1011374.g010
DOI:
10.1371/journal.ppat.1011374.g011
DOI:
10.1371/journal.ppat.1011374.s001
DOI:
10.1371/journal.ppat.1011374.s002
DOI:
10.1371/journal.ppat.1011374.s003
DOI:
10.1371/journal.ppat.1011374.s004
DOI:
10.1371/journal.ppat.1011374.s005
DOI:
10.1371/journal.ppat.1011374.s006
DOI:
10.1371/journal.ppat.1011374.s007
DOI:
10.1371/journal.ppat.1011374.s008
DOI:
10.1371/journal.ppat.1011374.s009
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2205412-1
Permalink