In:
PLOS Biology, Public Library of Science (PLoS), Vol. 19, No. 5 ( 2021-5-18), p. e3001229-
Abstract:
DNA methylation, chromatin accessibility, and gene expression represent different levels information in biological process, but a comprehensive multiomics analysis of the mammalian heart is lacking. Here, we applied nucleosome occupancy and methylome sequencing, which detected DNA methylation and chromatin accessibility simultaneously, as well as RNA-seq, for multiomics analysis of the 4 chambers of adult and fetal human hearts, and adult mouse hearts. Our results showed conserved region-specific patterns in the mammalian heart at transcriptome and DNA methylation level. Adult and fetal human hearts showed distinct features in DNA methylome, chromatin accessibility, and transcriptome. Novel long noncoding RNAs were identified in the human heart, and the gene expression profiles of major cardiovascular diseases associated genes were displayed. Furthermore, cross-species comparisons revealed human-specific and mouse-specific differentially expressed genes between the atria and ventricles. We also reported the relationship among multiomics and found there was a bell-shaped relationship between gene-body methylation and expression in the human heart. In general, our study provided comprehensive spatiotemporal and evolutionary insights into the regulation of gene expression in the heart.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001229
DOI:
10.1371/journal.pbio.3001229.g001
DOI:
10.1371/journal.pbio.3001229.g002
DOI:
10.1371/journal.pbio.3001229.g003
DOI:
10.1371/journal.pbio.3001229.g004
DOI:
10.1371/journal.pbio.3001229.g005
DOI:
10.1371/journal.pbio.3001229.s001
DOI:
10.1371/journal.pbio.3001229.s002
DOI:
10.1371/journal.pbio.3001229.s003
DOI:
10.1371/journal.pbio.3001229.s004
DOI:
10.1371/journal.pbio.3001229.s005
DOI:
10.1371/journal.pbio.3001229.s006
DOI:
10.1371/journal.pbio.3001229.s007
DOI:
10.1371/journal.pbio.3001229.s008
DOI:
10.1371/journal.pbio.3001229.s009
DOI:
10.1371/journal.pbio.3001229.s010
DOI:
10.1371/journal.pbio.3001229.s011
DOI:
10.1371/journal.pbio.3001229.s012
DOI:
10.1371/journal.pbio.3001229.s013
DOI:
10.1371/journal.pbio.3001229.s014
DOI:
10.1371/journal.pbio.3001229.s015
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2126773-X
Permalink