In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 18, No. 8 ( 2022-8-12), p. e1010348-
Abstract:
Epithelial cells secrete apical extracellular matrices to form protruding structures such as denticles, ridges, scales, or teeth. The mechanisms that shape these structures remain poorly understood. Here, we show how the actin cytoskeleton and a provisional matrix work together to sculpt acellular longitudinal alae ridges in the cuticle of adult C . elegans . Transient assembly of longitudinal actomyosin filaments in the underlying lateral epidermis accompanies deposition of the provisional matrix at the earliest stages of alae formation. Actin is required to pattern the provisional matrix into longitudinal bands that are initially offset from the pattern of longitudinal actin filaments. These bands appear ultrastructurally as alternating regions of adhesion and separation within laminated provisional matrix layers. The provisional matrix is required to establish these demarcated zones of adhesion and separation, which ultimately give rise to alae ridges and their intervening valleys, respectively. Provisional matrix proteins shape the alae ridges and valleys but are not present within the final structure. We propose a morphogenetic mechanism wherein cortical actin patterns are relayed to the laminated provisional matrix to set up distinct zones of matrix layer separation and accretion that shape a permanent and acellular matrix structure.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010348
DOI:
10.1371/journal.pgen.1010348.g001
DOI:
10.1371/journal.pgen.1010348.g002
DOI:
10.1371/journal.pgen.1010348.g003
DOI:
10.1371/journal.pgen.1010348.g004
DOI:
10.1371/journal.pgen.1010348.g005
DOI:
10.1371/journal.pgen.1010348.g006
DOI:
10.1371/journal.pgen.1010348.g007
DOI:
10.1371/journal.pgen.1010348.g008
DOI:
10.1371/journal.pgen.1010348.g009
DOI:
10.1371/journal.pgen.1010348.g010
DOI:
10.1371/journal.pgen.1010348.g011
DOI:
10.1371/journal.pgen.1010348.s001
DOI:
10.1371/journal.pgen.1010348.s002
DOI:
10.1371/journal.pgen.1010348.s003
DOI:
10.1371/journal.pgen.1010348.s004
DOI:
10.1371/journal.pgen.1010348.s005
DOI:
10.1371/journal.pgen.1010348.s006
DOI:
10.1371/journal.pgen.1010348.s007
DOI:
10.1371/journal.pgen.1010348.s008
DOI:
10.1371/journal.pgen.1010348.s009
DOI:
10.1371/journal.pgen.1010348.s010
DOI:
10.1371/journal.pgen.1010348.s011
DOI:
10.1371/journal.pgen.1010348.s012
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2186725-2
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