In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 16, No. 12 ( 2022-12-5), p. e0010977-
Abstract:
Plasmodium ovale curtisi and P . ovale wallikeri are both endemic in sub-Saharan Africa, the Middle East and Southeast Asia. Molecular surveillance data for drug resistance in P . ovale spp . is limited at present. We analysed polymorphisms in the podhfr , pocrt and pocytb genes of P . ovale spp . in 147 samples collected from travelers returning to China from Africa. Two podhfr mutations, S58R and S113N/T were detected in P . ovale curtisi with high/moderate frequencies of 52.17% and 17.39%, respectively. Evidence of positive selection (d N /d S = 2.41) was found for podhfr in P . ovale curtisi and decreased diversity ( H e ) of microsatellite markers flanking the mutant alleles suggests that selective sweeps have occurred for both. Mutations E34G (1.50%) and L43V (1.50%) in pocrt of P . ovale curtisi , and E34G (3.70%), I102M (1.80%) and V111F (1.80%) of P . ovale wallikeri were found at low frequencies. Mutations R66K (6.20%), R75K (11.63%) and R95K (3.88%) of pocytb were found in both P . ovale curtisi and P . ovale wallikeri . These results suggest that the podhfr gene of P . ovale curtisi may be subject to drug selection in Africa, warranting further attention. We observed significant differences in the prevalence and distribution of podhfr mutations between the two P . ovale species, suggestive of fundamental biological differences between them.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0010977
DOI:
10.1371/journal.pntd.0010977.g001
DOI:
10.1371/journal.pntd.0010977.g002
DOI:
10.1371/journal.pntd.0010977.g003
DOI:
10.1371/journal.pntd.0010977.t001
DOI:
10.1371/journal.pntd.0010977.t002
DOI:
10.1371/journal.pntd.0010977.t003
DOI:
10.1371/journal.pntd.0010977.s001
DOI:
10.1371/journal.pntd.0010977.s002
DOI:
10.1371/journal.pntd.0010977.s003
DOI:
10.1371/journal.pntd.0010977.s004
DOI:
10.1371/journal.pntd.0010977.s005
DOI:
10.1371/journal.pntd.0010977.s006
DOI:
10.1371/journal.pntd.0010977.s007
DOI:
10.1371/journal.pntd.0010977.s008
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2429704-5
Permalink