In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 10 ( 2022-10-21), p. e0275239-
Abstract:
We developed a novel multi-torsional mechanical stretch stress loading device for ligamentum flavum cells and evaluated its influence on the development of ligamentum flavum hypertrophy, a common cause of lumbar spinal canal stenosis. Materials and methods Stretch strength of the device was optimized by applying 5% and 15% MSS loads for 24, 48, and 72 h. A cytotoxicity assay of human ligamentum flavum cells was performed and the results were compared to control (0% stress). Inflammatory markers (interleukin [IL]-6, IL-8), vascular endothelial growth factor [VEGF] , and extracellular matrix (ECM)-regulating cytokines (matrix metalloproteinase [MMP]-1, MMP-3 and MMP-9, and tissue inhibitor of metalloproteinase [TIMP] -1 and TIMP-2) were quantified via enzyme-linked immunosorbent assay. Results Using our multi-torsional mechanical stretch stress loading device, 5% stress for 24 hour was optimal for ligamentum flavum cells. Under this condition, the IL-6 and IL-8 levels, VEGF level, and MMP-1, MMP-3, and TIMP-2 were significantly increased, compared to the control. Conclusion Using the novel multi-torsional mechanical stretch stress loading device we confirmed that, mechanical stress enhances the production of inflammatory cytokines and angiogenic factors, and altered the expression of ECM-regulating enzymes, possibly triggering ligamentum flavum hypertrophy.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0275239
DOI:
10.1371/journal.pone.0275239.g001
DOI:
10.1371/journal.pone.0275239.g002
DOI:
10.1371/journal.pone.0275239.g003
DOI:
10.1371/journal.pone.0275239.g004
DOI:
10.1371/journal.pone.0275239.g005
DOI:
10.1371/journal.pone.0275239.g006
DOI:
10.1371/journal.pone.0275239.g007
DOI:
10.1371/journal.pone.0275239.t001
DOI:
10.1371/journal.pone.0275239.s001
DOI:
10.1371/journal.pone.0275239.s002
DOI:
10.1371/journal.pone.0275239.s003
DOI:
10.1371/journal.pone.0275239.r001
DOI:
10.1371/journal.pone.0275239.r002
DOI:
10.1371/journal.pone.0275239.r003
DOI:
10.1371/journal.pone.0275239.r004
DOI:
10.1371/journal.pone.0275239.r005
DOI:
10.1371/journal.pone.0275239.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
Permalink