In:
PLOS ONE, Public Library of Science (PLoS), Vol. 15, No. 12 ( 2020-12-30), p. e0244499-
Kurzfassung:
The synthesis of a mitochondria-targeted derivative of the classical mitochondrial uncoupler carbonyl cyanide-m-chlorophenylhydrazone (CCCP) by alkoxy substitution of CCCP with n-decyl(triphenyl)phosphonium cation yielded mitoCCCP, which was able to inhibit the uncoupling action of CCCP, tyrphostin A9 and niclosamide on rat liver mitochondria, but not that of 2,4-dinitrophenol, at a concentration of 1–2 μM. MitoCCCP did not uncouple mitochondria by itself at these concentrations, although it exhibited uncoupling action at tens of micromolar concentrations. Thus, mitoCCCP appeared to be a more effective mitochondrial recoupler than 6-ketocholestanol. Both mitoCCCP and 6-ketocholestanol did not inhibit the protonophoric activity of CCCP in artificial bilayer lipid membranes, which might compromise the simple proton-shuttling mechanism of the uncoupling activity on mitochondria.
Materialart:
Online-Ressource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0244499
DOI:
10.1371/journal.pone.0244499.g001
DOI:
10.1371/journal.pone.0244499.g002
DOI:
10.1371/journal.pone.0244499.g003
DOI:
10.1371/journal.pone.0244499.g004
DOI:
10.1371/journal.pone.0244499.g005
DOI:
10.1371/journal.pone.0244499.g006
DOI:
10.1371/journal.pone.0244499.s001
DOI:
10.1371/journal.pone.0244499.r001
DOI:
10.1371/journal.pone.0244499.r002
DOI:
10.1371/journal.pone.0244499.r003
DOI:
10.1371/journal.pone.0244499.r004
DOI:
10.1371/journal.pone.0244499.r005
DOI:
10.1371/journal.pone.0244499.r006
Sprache:
Englisch
Verlag:
Public Library of Science (PLoS)
Publikationsdatum:
2020
ZDB Id:
2267670-3
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