In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 97, No. 22 ( 2000-10-24), p. 12138-12143
Abstract:
Embryonic development in Drosophila is characterized
by an early phase during which a cellular blastoderm is formed and gastrulation takes place, and by a later postgastrulation phase in
which key morphogenetic processes such as segmentation and organogenesis occur. We have focused on this later phase in
embryogenesis with the goal of obtaining a comprehensive analysis of the zygotic gene expression that occurs during development under normal
and altered environmental conditions. For this, a functional genomic approach to embryogenesis has been developed that uses high-density
oligonucleotide arrays for large-scale detection and quantification of gene expression. These oligonucleotide arrays were used for
quantitative transcript imaging of embryonically expressed genes under standard conditions and in response to heat shock. In embryos raised
under standard conditions, transcripts were detected for 37% of the 1,519 identified genes represented on the arrays, and highly
reproducible quantification of gene expression was achieved in all cases. Analysis of differential gene expression after heat shock
revealed substantial expression level changes for known heat-shock genes and identified numerous heat shock-inducible genes. These results
demonstrate that high-density oligonucleotide arrays are sensitive, efficient, and quantitative instruments for the analysis of large scale
gene expression in Drosophila embryos.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.210066997
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2000
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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