Publikationsdatum:
2024-06-12
Beschreibung:
To assess the thermal adaptation of microscopic stages of the kelp Laminaria digitata along latitudes, we conducted laboratory experiments on samples from six locations in the NE Atlantic (Spitsbergen (SPT), Tromsø (TRM), Bodø (BOD; all Norway), Helgoland (HLG; Germany), Roscoff (ROS) and Quiberon (QUI; both France)), spanning the species' entire distribution range. Gametophyte stock cultures from the Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research were used. Prior to the experiments, cultures were stored at 15°C in iron-free ½ Provasoli enriched seawater in 3-4 µmol photons/m²/s red light. In experiment 2, samples were subjected to (sub-) optimal low temperatures (0-15°C) for 21 days, to assess gametophyte survival, sporophyte formation and growth. During the experiments, samples were kept in 15 µmol photons/m²/s white light under a 16:8h light:dark cycle. Sporophyte growth rates both in length and in width were determined as follows: GR = (x2-x1)/(t2-t1), where x is the length or width (μm) and t is the time in weeks at time point 1 and 2.
Schlagwort(e):
Calculated; common garden experiment; Counting, Stereo Microscope; DATE/TIME; Event label; Experiment day; gametogenesis; growth; Hand picking; Identification; kelp; Laboratory experiment; Laminaria digitata, gametophyte survival; Laminaria digitata, sporophyte abundance; Laminaria digitata, sporophyte formation; Laminaria digitata, sporophyte growth rate in length; Laminaria digitata, sporophyte growth rate in width; Laminaria digitata, sporophyte length to width ratio; latitude; Local adaptation; North Atlantic; Northeast Atlantic; Origin; Quiberon_L_digitata_culture; Reproduction; Species; Spitsbergen_L_digitata_culture-2; Survival; Temperature; Temperature, water; Treatment: period; Tromsø_L_digitata_culture; Type of study; Uniform resource locator/link to reference; upper survival temperature
Materialart:
Dataset
Format:
text/tab-separated-values, 2070 data points
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