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  • Springer  (67)
  • PANGAEA  (8)
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  • 11
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    Experiment on marine fungus Microascus brevicaulis strain: Comparative growth curves of LF580 and LF580-M26 (2015)
    PANGAEA
    Details
    Publication Date: 2023-03-14
    Keywords: Biomass; Biomass, standard error; Experiment day; pH; pH, standard deviation; Species; Strain; Time in hours
    Type: Dataset
    Format: text/tab-separated-values, 288 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 12
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    Physico-chemical parameters and accession numbers from the Logatchev Hydrothermal Field, located 15°N on the Mid-Atlantic Ridge (2021)
    PANGAEA
    Details
    Publication Date: 2023-03-15
    Description: Hydrothermal fluid samples were retrieved during dives made by the remotely operated vehicle (ROV) QUEST (MARUM, University of Bremen) during the HYDROMAR I (M60/3, 2004) and HYDROMAR II (M64/2, 2005) cruises to the Logatchev hyrothermal field. The samples were retrieved using a pumped flow-through system (Kiel Pumping System KIPS) specially designed for the ROV QUEST. Once on board the ship, the liquids intended for microbiological studies were concentrated on 0.2 µm pore size polycarbonate filters (Sartorius) and stored at -20°C. The pH and sulfide concentrations were determined immediately after sample recovery. The pH was measured (Mettler electrodes with Ag/AgCl reference electrode) at 25°C in unfiltered sample aliquots. Sulfide concentrations were determined photometrically following the methylene blue method or, for samples with low concentrations, by voltammetry (Metrohm Application Bulletin 199/3e). Methane was analyzed on board by applying a purge and trap technique. For on-board measurements of dissolved hydrogen the water sample was degassed into a high-grade vacuum. Aliquots of the released gas were analyzed by gas chromatography (Thermo Electron Corporation Trace GC Ultra with a pulsed discharge detector). The abundance of bacterial and archaeal taxa was investigated by sequencing of the 16S rRNA gene. The diversity of the cbbL, cbbM and aclb was investigated by sequencing of the genes.
    Keywords: Accession number, genetics; ANME-2; Area/locality; chemoautotrophy; Epsilonproteobacteria; fluid chemistry; Gene name; Hydrogen; hydrogen oxidation; Hydrogen sulfide; LHF; Logatchev_Hydrothermal-Field; Logatchev hydrothermal field; Methane; Methanococcales; Mid-Atlantic Ridge; pH; Remote operated vehicle QUEST; ROVQ; sulfur oxidation; Temperature, water; ultramafic-hosted
    Type: Dataset
    Format: text/tab-separated-values, 26 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 13
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    Experiment on marine fungus Microascus brevicaulis strain (2015)
    PANGAEA
    In:  Supplement to: Kramer, Annemarie; Beck, Hans Christian; Kumar, Abhishek; Kristensen, Lars Peter; Imhoff, Johannes F; Labes, Antje (2015): Proteomic Analysis of Anti-Cancerous Scopularide Production by a Marine Microascus brevicaulis Strain and Its UV Mutant. PLoS ONE, 10(10), e0140047, https://doi.org/10.1371/journal.pone.0140047
    Details
    Publication Date: 2023-01-13
    Description: The marine fungus Microascus brevicaulis strain LF580 is a non-model secondary metabolite producer with high yields of the two secondary metabolites scopularide A and B, which exhibit distinct activities against tumour cell lines. A mutant strain was obtained using UV mutagenesis, showing besides higher production levels faster growth and differences in pellet formation. Comparative proteomics were applied to gain deeper understanding of the regulation of production and of the physiology of this fungus and its mutant. For this purpose, an optimised protein extraction protocol was established. Here, we show the first proteome study of a marine fungus. In total, 4759 proteins were identified. The central metabolic pathway of LF580 could be mapped by using KEGG pathway analysis and GO annotation. Using iTRAQ labelling, 318 proteins were shown to be significantly regulated in the mutant strain: 189 were down- and 129 upregulated. Proteomics are a powerful tool for the understanding of regulatory aspects: The differences on proteome level could be attributed to a limited nutrient availability in wild type strain due to a strong pellet formation. This information can be applied to optimisation on strain and process level. The linkage between nutrient limitation and pellet formation in the non-model fungus M. brevicaulis is in consensus with the knowledge on model organisms like Aspergillus niger and Penicillium chrysogenum.
    Type: Dataset
    Format: application/zip, 2 datasets
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 14
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    NMR spectrum of lindgomycin from a Marine Fungus of the Lindgomycetaceae (2016)
    PANGAEA
    In:  Supplement to: Wu, Bin; Wiese, Jutta; Labes, Antje; Kramer, Annemarie; Schmaljohann, Rolf; Imhoff, Johannes F (2015): Lindgomycin, an Unusual Antibiotic Polyketide from a Marine Fungus of the Lindgomycetaceae. Marine Drugs, 13(8), 4617-4632, https://doi.org/10.3390/md13084617
    Details
    Publication Date: 2023-01-13
    Description: An unusual polyketide with a new carbon skeleton, lindgomycin (1), and the recently described ascosetin (2) were extracted from mycelia and culture broth of different Lindgomycetaceae strains, which were isolated from a sponge of the Kiel Fjord in the Baltic Sea (Germany) and from the Antarctic. Their structures were established by spectroscopic means. In the new polyketide, two distinct domains, a bicyclic hydrocarbon and a tetramic acid, are connected by a bridging carbonyl. The tetramic acid substructure of compound 1 was proved to possess a unique 5-benzylpyrrolidine-2,4-dione unit. The combination of 5-benzylpyrrolidine-2,4-dione of compound 1 in its tetramic acid half and 3-methylbut-3-enoic acid pendant in its decalin half allow the assignment of a new carbon skeleton. The new compound 1 and ascosetin showed antibiotic activities with IC50 value of 5.1 (±0.2) µM and 3.2 (±0.4) µM, respectively, against methicillin-resistant Staphylococcus aureus.
    Type: Dataset
    Format: application/pdf, 49.3 kBytes
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 15
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    Experiment on marine fungus Microascus brevicaulis strain: Overview of Gene Ontology data of mutant strain LF580-M26 in comparision to wild type strain LF580 (2015)
    PANGAEA
    Details
    Publication Date: 2023-01-13
    Keywords: Accession number; Category; Comment; Description; Enzyme code; Gene Ontology term
    Type: Dataset
    Format: text/tab-separated-values, 1828 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 16
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    (Table 2) Hydrogen sulphide and methane content, and loss on ignition in sediments obtained in the Gotland Deep, Baltic Sea (1998)
    PANGAEA
    In:  Supplement to: Schmaljohann, Rolf; Piker, Levent; Imhoff, Johannes F (1998): The distribution of methane and hydrogen sulfide in basin sediments of the central and southern Baltic Sea. Meyniana, 50, 191-211, https://doi.org/10.2312/meyniana.1998.50.191
    Details
    Publication Date: 2024-05-21
    Description: The distribution of methane and hydrogen sulfide concentrations in sediments of various basins of the Baltic Sea was investigated during 4 cruises in 1995 and 1996. Significant differences in the concentrations of both compounds were recorded between the basins and also between different areas within the Gotland Deep. High-methane sediments with distinctly increasing concentrations from the surface to deeper layers were distinguished from low-methane sediments without a clear gradient. Methane concentrations exhibited a fair correlation with the sediment accumulation rate, determined by measuring the total thickness of the post-Ancylus Holocene sequence on echosounding profiles in the Gotland Deep. Only weak correlations were observed with the content of organic matter in the surface layers of the sediments. Hydrogen sulfide concentrations in the sediments showed a positive correlation with methane concentrations, but, in contrast to methane concentrations, were strongly influenced by the transition from oxic to anoxic conditions in the water column between 1995 and 1996. Sediments in the deepest part of the Gotland Basin (〉237 m water depth), covering an area of approximately 35 km**2, were characterized by especially high accumulation rates (〉70 cm/ka) and high methane and hydrogen sulfide contents. Concentrations of these compounds decreased rapidly towards the slope of the basin.
    Keywords: AL93; Arcona Basin, Baltic Sea; BY15A; Date; Depth, bottom/max; DEPTH, sediment/rock; Depth, top/min; Direction; Distance; Elevation 2; Event label; GC; GotlDeep; Gravity corer; Hydrogen sulfide; Layer thickness; Loss on ignition; Methane, sediment; Sample code/label
    Type: Dataset
    Format: text/tab-separated-values, 613 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 17
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    Physico-chemical parameters and accession numbers from the Lilliput hydrothermal field, southern Mid-Atlantic Ridge (9°S) (2021)
    PANGAEA
    Details
    Publication Date: 2024-06-26
    Description: Hydrothermal vent fluids were sampled with a pumped flow-through system with PFA sampling bottles (Kiel Pumping System KIPS) by the ROV QUEST. Fuid chemical parameters were determined immediately after sample recovery. Ph was measured at 20°C in unfiltered sample aliquots. Sulfide concentrations were measured colorimetrcally following the methylene blue method. Fe(II) and Fe(III) was measured colorimetrically with the phenantroline assay. Total Fe was measured by reducing all Fe(III) to Fe(II) with ascorbic acid. Fe(III) was calculated from the diffrence between Fe(II) and total Fe. Light dissolved hydrocharbons were analysed on board applying a purge and trap technique. Dissolved hydrogen was mesured onboard by a GC equipped with a PDD. Stable carbon isotope signatures of methane were analysed using coupled gas chromatography-combustion-isotope ration mass spectrometry. The abundance of bacterial and archaeal taxa was investigated by sequencing of the 16S rRNA gene. The diversity of the cbbL, cbbM and aclb was investigated by sequencing of the genes.
    Keywords: Accession number, genetics; Area/locality; Candidate Divisions; Endmember fluid portion; Epsilonproteobacteria; fish; Gene name; Hydrogen; Hydrogen sulfide; Iron 2+; Iron 3+; low-temperature hydrothermal fluids; M64/1; M64/1_Lilliput-hydrothermal-field; M68/1; M68/1_Lilliput-hydrothermal-field; MARSUED2; MARSUED3; Meteor (1986); Methane; microbial community; Mid-Atlantic Ridge; pH; Remote operated vehicle QUEST; ROVQ; Sampling date; sulfur oxidation; Temperature, water; Thiomicrospira; δ13C, methane
    Type: Dataset
    Format: text/tab-separated-values, 42 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 18
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    NMR spectrum of lindgomycin (2016)
    PANGAEA
    In:  EPIC3Bremerhaven, PANGAEA
    Details
    Publication Date: 2016-01-07
    Repository Name: EPIC Alfred Wegener Institut
    Type: PANGAEA Documentation , notRev
    Format: application/pdf
    Location Call Number Limitation Availability
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    PAPER (OA)
  • 19
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    Subtercola vilae sp. nov., a novel actinobacterium from an extremely high-altitude cold volcano lake in Chile (2018)
    Springer
    In:  Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, 111 . pp. 955-963.
    Details
    Publication Date: 2021-02-08
    Description: A novel actinobacterium, strain DB165T, was isolated from cold waters of Llullaillaco Volcano Lake (6170 m asl) in Chile. Phylogenetic analysis based on 16S rRNA gene sequences identified strain DB165T as belonging to the genus Subtercola in the family Microbacteriaceae, sharing 97.4% of sequence similarity with Subtercola frigoramans DSM 13057T, 96.7% with Subtercola lobariae DSM 103962T, and 96.1% with Subtercola boreus DSM 13056T. The cells were observed to be Gram-positive, form rods with irregular morphology, and to grow best at 10–15 °C, pH 7 and in the absence of NaCl. The cross-linkage between the amino acids in its peptidoglycan is type B2γ; 2,4-diaminobutyric acid is the diagnostic diamino acid; the major respiratory quinones are MK-9 and MK-10; and the polar lipids consist of phosphatidylglycerol, diphosphatidylglycerol, 5 glycolipids, 2 phospholipids and 5 additional polar lipids. The fatty acid profile of DB165T (5% 〉) contains iso-C14:0, iso-C16:0, anteiso-C15:0, anteiso-C17:0, and the dimethylacetal iso-C16:0 DMA. The genomic DNA G+C content of strain DB165T was determined to be 65 mol%. Based on the phylogenetic, phenotypic, and chemotaxonomic analyses presented in this study, strain DB165T (= DSM 105013T = JCM 32044T) represents a new species in the genus Subtercola, for which the name Subtercola vilae sp. nov. is proposed.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1007/s10482-017-0994-4
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    OceanRep: Article in a Scientific Journal - peer-reviewed
  • 20
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    New insights into the metabolic potential of the phototrophic purple bacterium Rhodopila globiformis DSM 161T from its draft genome sequence and evidence for a vanadium-dependent nitrogenase (2018)
    Springer
    In:  Archives of Microbiology, 200 . pp. 847-857.
    Details
    Publication Date: 2021-02-08
    Description: Rhodopila globiformis: is the most acidophilic anaerobic anoxygenic phototrophic purple bacterium and was isolated from a warm acidic sulfur spring in Yellowstone Park. Its genome is larger than genomes of other phototrophic purple bacteria, containing 7248 Mb with a G + C content of 67.1% and 6749 protein coding and 53 RNA genes. The genome revealed some previously unknown properties such as the presence of two sets of structural genes pufLMC for the photosynthetic reaction center genes and two types of nitrogenases (Mo-Fe and V-Fe nitrogenase), capabilities of autotrophic carbon dioxide fixation and denitrification using nitrite. Rhodopila globiformis assimilates sulfate and utilizes the C1 carbon substrates CO and methanol and a number of organic compounds, in particular, sugars and aromatic compounds. It is among the few purple bacteria containing a large number of pyrroloquinoline quinone-dependent dehydrogenases. It has extended capacities to resist stress by heavy metals, demonstrates different resistance mechanisms to antibiotics, and employs several toxin/antitoxin systems.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1007/s00203-018-1489-z
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    OceanRep: Article in a Scientific Journal - peer-reviewed
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