In:
Journal of Leukocyte Biology, Oxford University Press (OUP), Vol. 53, No. 4 ( 1993-04-01), p. 399-403
Abstract:
Developmental delays, which impair antibacterial host defense, are present in the neutrophil system of human preterm neonates. We hypothesized that diminished production of interleukin-8 (IL-8), a neutrophil chemotactic peptide, might in part be responsible for these defects. To test this, monocytes from the blood of preterm neonates, term neonates, and adults were isolated immunologically by “negative panning” and subsequently stimulated with interleukin-1α (IL-lα), tumor necrosis factor a (TNF-α), or lipopolysaccharide (LPS), after which IL-8 levels in the supernatants were measured by ELISA. Total cellular RNA was extracted and IL-8 mRNA was assessed by Northern blotting and by competitive polymerase chain reaction (PCR) analyses. After stimulation with IL-lα, IL-8 accumulation was lowest in supernatants of monocytes from preterm neonates, intermediate in supernatants of monocytes from term neonates and greatest from monocytes of adults. Similarly, when stimulated with TNF-α or LPS, monocytes from preterm neonates produced less IL-8 than cells from term neonates and adults. These differences in IL-8 concentrations paralleled differences in IL-8 mRNA expression. J. Leukoc. Biol. 53: 399–403; 1993.
Type of Medium:
Online Resource
ISSN:
0741-5400
,
1938-3673
DOI:
10.1002/jlb.53.4.399
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
1993
detail.hit.zdb_id:
2026833-6
SSG:
12
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