In:
Journal of AOAC INTERNATIONAL, Oxford University Press (OUP), Vol. 91, No. 6 ( 2008-11-01), p. 1499-1506
Abstract:
An efficient method was developed for simultaneous determination of 13 quinolonesnamely, enoaxacin (ENO), marbofloxacin (MAR), ciprofloxacin (CIP), norfloxacin (NOR), ofloxacin (OFL), pefloxacin methanesulfonate (PEF), danofloxacin (DAN), enrofloxacin (ENR), lomefloxacin (LOM), difloxacin (DIF), sarafloxacin (SAR), oxolinic acid (OXO), and flumequine (FLU)in eggs by column liquid chromatography/electrospray ionization-tandem mass spectrometry. Samples were extracted with a phosphoric acidphosphate buffer followed by purification with a solid-phase extraction cartridge. Recoveries for the 13 quinolones were 6793 with intraday and interday coefficients of variation ranging from 4 to 9 and 2 to 18, respectively. The limit of determination was 0.05 g/kg for OXO and FLU; 0.1 g/kg for MAR, OFL, CIP, LOM, DAN, SAR, DIF, NOR, and ENR; and 0.2 g/kg for ENO and PEF. The method was also applied to study the depletion of PEF in eggs. The concentration of PEF increased and reached a maximum value on the third day, and then decreased rapidly until it could not be detected on day 32; its metabolite NOR was detectable on the second day, and then reached a maximum on the sixth day, after which it could not be detected until day 15.
Type of Medium:
Online Resource
ISSN:
1060-3271
,
1944-7922
DOI:
10.1093/jaoac/91.6.1499
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2008
Permalink