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  • 1
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    Online Resource
    Stimulatory guanine nucleotide binding protein activity in the erythrocyte membrane of patients with pseudohypoparathyroidism type I and related disorders
    Oxford University Press (OUP) ; 1986
    In:  Acta Endocrinologica Vol. 111, No. 4 ( 1986-04), p. 507-515
    Details
    In: Acta Endocrinologica, Oxford University Press (OUP), Vol. 111, No. 4 ( 1986-04), p. 507-515
    Abstract: Abstract. The activity of stimulatory guanine nucleotide regulatory protein (Ns) in the erythrocyte membrane was assayed by the reconstitution method using plasma membrane of cyc − S49 mouse lymphoma cells in 18 patients with type I pseudohypoparathyroidism (PHP-I), 2 with pseudopseudohypoparathyroidism (PPHP) and 30 normal subjects, in parallel with other clinical parameters. The Ns activity as expressed by per cent of pooled standard (mean ± se ) was 78.9 ±6.1 in PHP-I patients, which was significantly lower ( P 〈 0.01) than the value in normal subjects, 99.5 ± 2.4. In PHP-I patients, the Ns activities (Y) were in significant correlation with three clinical parameters examined (X), i.e., with body height in standard deviation score from the mean of the normal population at the corresponding age, Y = 89.4 + 10.4X (r = 0.616, P 〈 0.01); with urinary cAMP excretion in relation to creatinine [cAMP(nmol)/Cr(mg)], Y = 56.3 + 7.2X (r = 0.501, P 〈 0.05); and with TSH levels in plasma (μU/ml), Y = 129–3X (r = 0.639, P 〈 0.01). The Ns activities of PPHP were as low as 53.8 and 60.0. The decrease of Ns activity in the cell membrane may be implicated in the development of the clinical symptoms such as short stature, decrease in urinary excretion of cAMP and latent or manifest primary hypothyroidism in PHP-I and possibly in skeletal abnormality in PPHP.
    Type of Medium: Online Resource
    ISSN: 0804-4643 , 1479-683X
    URL: Article
    DOI: 10.1530/acta.0.1110507
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Oxford University Press (OUP)
    Publication Date: 1986
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  • 2
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    Online Resource
    Properties of Rat Bone Inorganic Pyrophosphatase Studied by Means of 32P-Radioassay
    Oxford University Press (OUP) ; 1971
    In:  The Journal of Biochemistry Vol. 70, No. 6 ( 1971-12), p. 1003-1010
    Details
    In: The Journal of Biochemistry, Oxford University Press (OUP), Vol. 70, No. 6 ( 1971-12), p. 1003-1010
    Type of Medium: Online Resource
    ISSN: 1756-2651 , 0021-924X
    URL: Article
    DOI: 10.1093/oxfordjournals.jbchem.a129709
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 1971
    detail.hit.zdb_id: 2009977-0
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  • 3
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    Targeting Nrf2-antioxidant signalling reverses acquired cabazitaxel resistance in prostate cancer cells
    Oxford University Press (OUP) ; 2021
    In:  The Journal of Biochemistry Vol. 170, No. 1 ( 2021-09-22), p. 89-96
    Details
    In: The Journal of Biochemistry, Oxford University Press (OUP), Vol. 170, No. 1 ( 2021-09-22), p. 89-96
    Abstract: Prostate cancer is known to have a relatively good prognosis, but long-term hormone therapy can lead to castration-resistant prostate cancer (CRPC). Cabazitaxel, a second-generation taxane, has been used for the CRPC treatment, but its tolerance is an urgent problem to be solved. In this study, to elucidate the acquisition mechanism of the cabazitaxel-resistance, we established cabazitaxel-resistant prostate cancer 22Rv1 (Cab-R) cells, which exhibited ∼sevenfold higher LD50 against cabazitaxel than the parental 22Rv1 cells. Cab-R cells showed marked increases in nuclear accumulation of NF-E2 related factor 2 (Nrf2) and expression of Nrf2-inducible antioxidant enzymes compared to 22Rv1 cells, suggesting that Nrf2 signalling is homeostatically activated in Cab-R cells. The cabazitaxel sensitivity of Cab-R cells was enhanced by silencing of Nrf2, and that of 22Rv1 cells was reduced by activation of Nrf2. Halofuginone (HF) has been recently identified as a potent Nrf2 synthetic inhibitor, and its treatment of Cab-R cells not only suppressed the Nrf2 signalling by decreasing both nuclear and cytosolic Nrf2 protein levels, but also significantly augmented the cabazitaxel sensitivity. Thus, inhibition of Nrf2 signalling may be effective in overcoming the cabazitaxel resistance in prostate cancer cells.
    Type of Medium: Online Resource
    ISSN: 0021-924X , 1756-2651
    URL: Article
    DOI: 10.1093/jb/mvab025
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2021
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  • 4
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    Specific receptors for epidermal growth factor in human bone tumour cells and its effect on synthesis of prostaglandin E2 by cultured osteosarcoma cell line
    Oxford University Press (OUP) ; 1984
    In:  Acta Endocrinologica Vol. 107, No. 1 ( 1984-09), p. 125-130
    Details
    In: Acta Endocrinologica, Oxford University Press (OUP), Vol. 107, No. 1 ( 1984-09), p. 125-130
    Abstract: Abstract. Using tumour cell lines derived from human bone tumours, specific binding sites for epidermal growth factor (EGF), a potent growth stimulator in many tissues, and its effect on synthesis of prostaglandin (PG) E 2 , a potent bone-resorbing factor, by cultured osteosarcoma cell line were studied. Three tumour cell lines, one osteosarcoma (HOSO) and two giant cell tumours of the bone (G-1 and G-2), all possessed specific binding sites for 125 I-labelled EGF: the apparent dissociation constant was ~4–10 × 10 −10 m and the maximal binding capacity was 50 000–80 000 sites/cell. EGF had no mitogenic effect in these cell lines. However, these cell lines did not have specific binding sites for 125 I-labelled parathyroid hormone (PTH) or calcitonin. HOSO line produced and secreted PGE 2 into medium, while no significant amount of PGE 2 was demonstrated in G-1 or G-2 line. EGF significantly stimulated PGE 2 production in HOSO line in a dose-dependent manner (0.5–50 ng/ml); its stimulatory effect was completely abolished by indomethacin, an inhibitor of PG biosynthesis. Exogenous PGE 1 significantly stimulated cyclic AMP formation in HOSO line, whereas PGF 2α , PTH, calcitonin, or EGF had no effect. None of these calcium-regulating hormones affected cyclic AMP generation in either G-1 or G-2 line. These data indicate that human bone tumour cells have specific EGF receptors unrelated to cell growth, and suggest that EGF may be involved in bone resorption through a PGE 2 -mediated process in human osseous tissues.
    Type of Medium: Online Resource
    ISSN: 0804-4643 , 1479-683X
    URL: Article
    DOI: 10.1530/acta.0.1070125
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Oxford University Press (OUP)
    Publication Date: 1984
    detail.hit.zdb_id: 1485160-X
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  • 5
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    Online Resource
    Concentrations of immunoreactive tonin in submandibular glands and saliva of the rat
    Oxford University Press (OUP) ; 1986
    In:  Acta Endocrinologica Vol. 111, No. 1 ( 1986-01), p. 140-144
    Details
    In: Acta Endocrinologica, Oxford University Press (OUP), Vol. 111, No. 1 ( 1986-01), p. 140-144
    Abstract: Abstract. The submandibular gland (SMG) of the rat contains tonin, an enzyme of serine proteases, which specifically cleaves angiotensinogen and angiotensin I to yield angiotensin II directly. Using a specific RIA for rat tonin, the present study was performed to examine the concentrations of immunoreactive (IR) tonin in SMG of normal Wistar male rats with various ages, those in SMG and saliva of normal adult males and females, and also its SMG levels of castrated males and testosterone-treated females. The concentrations of IR-tonin in SMG of immature 4 week old male rats were very low but rose exponentially with increasing age to reach adult levels (3–6 μg/mg wet tissue) in male rats after 6 week old or more. SMG of the adult male rat contained 10-fold more IR-tonin than that of the female rat and male saliva contained 5-fold more IR-tonin than that of females. The concentrations of SMG IR-tonin in the castrated males fell to about one-tenth the levels of normal males, whereas SMG IR-tonin levels in the testosterone-treated females increased about ten times more than those of normal females. These data confirm the sexual dimorphism of rat SMG with respect to the concentrations of tonin and indicate that androgens play an important role in regulating the synthesis and/or storage of tonin in rat SMG. It is also suggested that tonin is secreted into saliva in concentrations reflecting its SMG levels. However, biological significance of tonin in SMG and saliva remains obscure.
    Type of Medium: Online Resource
    ISSN: 0804-4643 , 1479-683X
    URL: Article
    DOI: 10.1530/acta.0.1110140
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Oxford University Press (OUP)
    Publication Date: 1986
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  • 6
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    Online Resource
    Effect of ovine corticotrophin releasing factor, bromocriptine, and dopamine on release of ACTH and β-endorphin in a patient with Cushing's disease
    Oxford University Press (OUP) ; 1985
    In:  Acta Endocrinologica Vol. 109, No. 1 ( 1985-05), p. 7-12
    Details
    In: Acta Endocrinologica, Oxford University Press (OUP), Vol. 109, No. 1 ( 1985-05), p. 7-12
    Abstract: Abstract. Release of immunoreactive ACTH and β-endorphin (β-EP) in response to corticotrophin-releasing factor (CRF) and dopaminergic agents was studied in vivo and in vitro in a patient with Cushing's disease. Iv administration of synthetic ovine (o) CRF significantly stimulated plasma ACTH release, accompanied by increase of plasma cortisol levels. Oral administration of bromocriptine significantly suppressed plasma cortisol levels. Although reduced responses of plasma ACTH and cortisol to o-CRF was observed 1 month after removal of the pituitary adenoma, these normalized 6 months after operation. In vitro perifusion of the pituitary adenoma obtained by surgery revealed that o-CRF also stimulated ACTH and β-EP release in a dose-responsive manner (10 −9 m –10 −5 m ) and that dopamine suppressed their basal secretion. Gel exclusion chromatography of the perfusates showed that the predominant component of ACTH and β-EP before and after o-CRF stimulation coeluted with standard ACTH and β-EP, respectively. The present data suggest that o-CRF is a potent secretagogue for ACTH and β-EP release from the human pituitary adenoma causing Cushing's disease and that ACTH secretion from certain adenomas, possibly originating from the intermediate lobe of the pituitary gland, is partly regulated by a dopaminergic mechanism.
    Type of Medium: Online Resource
    ISSN: 0804-4643 , 1479-683X
    URL: Article
    DOI: 10.1530/acta.0.1090007
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Oxford University Press (OUP)
    Publication Date: 1985
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  • 7
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    Online Resource
    Specific binding sites for epidermal growth factor and its effect on human chorionic gonadotrophin secretion by cultured tumour cell lines: comparison between trophoblastic and non-trophoblastic cells
    Oxford University Press (OUP) ; 1982
    In:  Acta Endocrinologica Vol. 101, No. 2 ( 1982-10), p. 281-286
    Details
    In: Acta Endocrinologica, Oxford University Press (OUP), Vol. 101, No. 2 ( 1982-10), p. 281-286
    Abstract: Using human trophoblastic (SCH) and non-trophoblastic (HeLa S 3 ) tumour cell lines, specific binding sites for epidermal growth factor (EGF), a potent stimulator of growth in many tissues, and its effect on secretion of human chorionic gonadotrophin (hCG) and/ or its subunits were compared between these two tumour cells. Both SCH and HeLa S 3 cells possessed two populations of specific binding sites for 125 I-labelled EGF: the high affinity (K d ∼10 −10 m ) and the low affinity (K d ∼ 7 × 10 −10 m ) system. Tetradecanoyl phorbol acetate (TPA), a tumour promotor, showed a potent competitor of labelled tracer binding to its receptor sites in both cell lines. EGF stimulated both hCG-α and hCG and/or hCG-β secretion in a dose-responsive manner from SCH cells, whereas it had no effect on hCG-α secretion from HeLa S 3 cells. In contrast, dibutyryl cyclic AMP plus theophylline, a phosphodiesterase inhibitor, enhanced hCG-α secretion from both cells, while TPA had no effect in either cells. These data suggest that EGF may play a physiological role in hCG secretion from trophoblastic tissues and that the mechanism by which hCG and/or its subunits are secreted may differ between trophoblastic and non-trophoblastic tumour cells.
    Type of Medium: Online Resource
    ISSN: 0804-4643 , 1479-683X
    URL: Article
    DOI: 10.1530/acta.0.1010281
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Oxford University Press (OUP)
    Publication Date: 1982
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  • 8
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    Online Resource
    EFFECTS OF ALPHA- AND BETA-ADRENERGIC BLOCKERS ON BINDING OF THYROTROPHIN TO FAT CELL MEMBRANE
    Oxford University Press (OUP) ; 1979
    In:  Acta Endocrinologica Vol. 90, No. 2 ( 1979-02), p. 240-248
    Details
    In: Acta Endocrinologica, Oxford University Press (OUP), Vol. 90, No. 2 ( 1979-02), p. 240-248
    Abstract: Alpha- (phentolamine) and beta-adrenergic blocking agents (propranolol) and quinidine similarly enhance the specific binding of TSH to the guinea pig fat cell membranes over the concentration range of 2 × 10 −4 to 4 × 10 ™3 m , increasing the binding affinity of TSH to the membranes. The percentage bound increased from 8 % in the absence of agents to 32 % (phentolamine), 29% (propranolol) and 24% (quinidine), respectively in the presence of these agents (1.5 × 10 −3 m ). Each minimal detectable quantity of TSH was approximately 10 μU per tube in the presence of these agents (10 −3 m ) as compared to 100 μU per tube in their absence. Both phentolamine and propranolol appeared to enhance the TSH binding to fat cell membranes through membrane-active, non-specific effects besides their alpha- and beta-adrenergic blocking activities.
    Type of Medium: Online Resource
    ISSN: 0804-4643 , 1479-683X
    URL: Article
    DOI: 10.1530/acta.0.0900240
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Oxford University Press (OUP)
    Publication Date: 1979
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  • 9
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    Online Resource
    Individual and combined effects of intact PTH, amino-terminal, and a series of truncated carboxyl-terminal PTH fragments on alkaline phosphatase activity in dexamethasone-treated rat osteoblastic osteosarcoma cells, ROS 17/2.8
    Oxford University Press (OUP) ; 1993
    In:  Acta Endocrinologica Vol. 128, No. 4 ( 1993-04), p. 367-372
    Details
    In: Acta Endocrinologica, Oxford University Press (OUP), Vol. 128, No. 4 ( 1993-04), p. 367-372
    Abstract: The individual and combined effects of intact PTH, amino-terminal, and a series of truncated carboxyl-terminal PTH fragments on alkaline phosphatase activity were examined in dexamethasone-treated rat osteoblastic osteosarcoma cells ROS 17/2.8. Dexamethasone-induced alkaline phosphatase activity was inhibited not only by hPTH(1–84) and amino-terminal PTH fragment hPTH(1–34), but also by carboxyl-terminal PTH fragment hPTH(69–84) in a dose-related fashion. At 10 −7 mol/1, hPTH(1–84) completely abolished dexamethasone-induced alkaline phosphatase activity, while hPTH(1–34) and hPTH(69–84) reduced alkaline phosphatase activity to 0.16±0.02 and 0.80±0.03 fold, respectively, of the control value obtained in the absence of PTH peptides. The combination of hPTH(1–34) and hPTH(69—84) resulted in reduction of alkaline phosphatase activity to the level obtained by hPTH(1-84). The shorter carboxyl-terminal PTH fragment hPTH(71–84) did not affect alkaline phosphatase activity or modulate the action of hPTH(1–34). The longer carboxyl-terminal PTH fragment hPTH(53-84) stimulated alkaline phosphatase activity up to 1.23±0.03 fold and partially blunted the inhibitory effect of hPTH(1–34) on alkaline phosphatase activity. These findings suggest that carboxyl-terminal PTH fragments could exert diverse effects on the target cells, depending on the length of deletion of amino-terminal amino acids of PTH molecule, and interact with amino-terminal PTH fragment. The two amino-terminal amino acids of hPTH(69–84) and the 53–68 portion of hPTH(53–84) might be responsible for the respective inhibitory and stimulatory effects of the peptides on alkaline phosphatase activity.
    Type of Medium: Online Resource
    ISSN: 0804-4643 , 1479-683X
    URL: Article
    DOI: 10.1530/acta.0.1280367
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Oxford University Press (OUP)
    Publication Date: 1993
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  • 10
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    Online Resource
    A CASE OF CUSHING'S SYNDROME STUDIED BY URINARY CORTICOTROPHIN ASSAY
    Oxford University Press (OUP) ; 1963
    In:  Acta Endocrinologica Vol. 42, No. 2 ( 1963-02), p. 163-167
    Details
    In: Acta Endocrinologica, Oxford University Press (OUP), Vol. 42, No. 2 ( 1963-02), p. 163-167
    Abstract: A 20 year old female developed typical features of Cushing's syndrome, with increased urinary and plasma 17-OHCS, which showed a distinct diurnal variation, exaggerated response to exogenous ACTH, and suppression of response to 3 mg dexamethasone daily. Total adrenalectomy was performed for bilateral adrenal hyperplasia. ACTH in urine showed a distinct increase before and after operation. More than 30 mg of cortisol was required to suppress the elevated urinary ACTH, suggesting an abnormal response of the pituitary gland to exogenous steroid.
    Type of Medium: Online Resource
    ISSN: 0804-4643 , 1479-683X
    URL: Article
    DOI: 10.1530/acta.0.0420163
    RVK:
    WA 15000
    Language: Unknown
    Publisher: Oxford University Press (OUP)
    Publication Date: 1963
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