Publication Date:
2012-10-10
Description:
We developed a highly scalable ‘shotgun’ DNA synthesis technology by utilizing microchip oligonucleotides, shotgun assembly and next-generation sequencing technology. A pool of microchip oligonucleotides targeting a penicillin biosynthetic gene cluster were assembled into numerous random fragments, and tagged with 20 bp degenerate barcode primer pairs. An optimal set of error-free fragments were identified by high-throughput DNA sequencing, selectively amplified using the barcode sequences, and successfully assembled into the target gene cluster.
Keywords:
Synthetic Biology and Assembly Cloning
Print ISSN:
0305-1048
Electronic ISSN:
1362-4962
Topics:
Biology
Permalink