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  • Ovid Technologies (Wolters Kluwer Health)  (21)
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  • Ovid Technologies (Wolters Kluwer Health)  (21)
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Fachgebiete(RVK)
  • 1
    Online-Ressource
    Online-Ressource
    Assessment of Antimicrobial Activity of Dehydrated Amniotic Membrane in Infectious Keratitis: A Small Retrospective Case Series and In Vitro Study
    Ovid Technologies (Wolters Kluwer Health) ; 2021
    In:  Cornea Vol. 40, No. 10 ( 2021-10), p. 1348-1352
    Details
    In: Cornea, Ovid Technologies (Wolters Kluwer Health), Vol. 40, No. 10 ( 2021-10), p. 1348-1352
    Kurzfassung: To describe a small case series of infectious keratitis with poor visual outcomes after amniotic membrane (AM) placement and to prospectively evaluate whether AM demonstrates antibacterial activity in vitro against pathogens commonly isolated from infectious corneal ulcers. Methods: A retrospective case series and in vitro study of antibacterial activity of dehydrated AM using disk diffusion and measurement of inhibitory zones for bacterial assessment and inverted microscopy analysis for Acanthamoeba sp. growth. Results: Three cases of known etiology infectious keratitis are described where the clinical presentation worsened after treatment with AM. In vitro analysis of dehydrated AM, with and without a soft contact lens, demonstrated no inhibition of growth against Pseudomonas aeruginosa or Streptococcus pneumoniae. There was minimal growth inhibition of Staphylococcus aureus , although these zones of inhibition were much smaller than that surrounding the positive control. For Acanthamoeba sp., solubilized, dehydrated AM did not alter cyst density. Conclusions: In an in vitro analysis, dehydrated AM did not provide evidence for a potentially clinically meaningful antibacterial effect against organisms commonly isolated from corneal ulcers.
    Materialart: Online-Ressource
    ISSN: 0277-3740
    URL: Article
    DOI: 10.1097/ICO.0000000000002627
    RVK:
    XA 37258
    RVK:
    XA 10000
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2021
    ZDB Id: 2045943-9
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 2
    Online-Ressource
    Online-Ressource
    Remote corneal suturing wet lab: microsurgical education during the COVID-19 pandemic
    Ovid Technologies (Wolters Kluwer Health) ; 2020
    In:  Journal of Cataract and Refractive Surgery Vol. 46, No. 12 ( 2020-12), p. 1667-1673
    Details
    In: Journal of Cataract and Refractive Surgery, Ovid Technologies (Wolters Kluwer Health), Vol. 46, No. 12 ( 2020-12), p. 1667-1673
    Kurzfassung: To study the feasibility and efficacy of a new remote wet lab for microsurgical education using a corneal suturing task. Setting: Department of Ophthalmology, University of California San Francisco, San Francisco, California, USA. Design: Prospective randomized controlled study. Methods: Ten ophthalmology residents were stratified by postgraduate year and randomized to perform a corneal suturing task consisting of placing the 4 cardinal sutures for a penetrating keratoplasty in porcine eyes with or without remote ophthalmology attending feedback. Subsequently, both groups repeated the same task without remote feedback to test whether initial remote feedback affected subsequent performance. Finally, the group without feedback was crossed over to repeat the same corneal suturing task with remote feedback. The effectiveness of the remote wet lab was assessed subjectively by survey and objectively by grading each suture pass. Results: Resident-reported comfort with corneal suturing improved significantly after the remote wet lab for all residents. Residents and attendings rated the remote wet lab as equally or more effective compared with previous in-person wet labs and overall effective in corneal suturing. Attendings rated the remote wet lab as effective in multiple domains of microsurgical education using a modified microsurgical global rating scale. Objective corneal suturing performance was similar for both groups. Conclusions: The remote wet lab was feasible and effective for training ophthalmology residents in corneal suturing. This represents a new social distancing compliant platform for microsurgical education during the COVID-19 pandemic.
    Materialart: Online-Ressource
    ISSN: 0886-3350 , 1873-4502
    URL: Article
    DOI: 10.1097/j.jcrs.0000000000000374
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2020
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 3
    Online-Ressource
    Online-Ressource
    Reply: Remote corneal suturing wet lab: microsurgical education during the COVID-19 pandemic.
    Ovid Technologies (Wolters Kluwer Health) ; 2021
    In:  Journal of Cataract and Refractive Surgery Vol. 47, No. 4 ( 2021-04), p. 556-557
    Details
    In: Journal of Cataract and Refractive Surgery, Ovid Technologies (Wolters Kluwer Health), Vol. 47, No. 4 ( 2021-04), p. 556-557
    Materialart: Online-Ressource
    ISSN: 0886-3350 , 1873-4502
    URL: Article
    DOI: 10.1097/j.jcrs.0000000000000619
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2021
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 4
    Online-Ressource
    Online-Ressource
    Impact of Sample Collection Order on the Diagnostic Performance of Metagenomic Deep Sequencing for Infectious Keratitis
    Ovid Technologies (Wolters Kluwer Health) ; 2022
    In:  Cornea Vol. 41, No. 1 ( 2022-01), p. 39-44
    Details
    In: Cornea, Ovid Technologies (Wolters Kluwer Health), Vol. 41, No. 1 ( 2022-01), p. 39-44
    Kurzfassung: The purpose of this article was to evaluate the impact of sample collection order on the diagnostic yield of metagenomic deep sequencing (MDS) for determining the causative pathogen in infectious keratitis. Methods: We performed a cross-sectional diagnostic test evaluation among subjects with infectious keratitis at Aravind Eye Hospital in Madurai, India. All subjects underwent corneal scrapings of the affected eye to obtain potassium hydroxide smear, Gram stain, bacterial culture, and fungal culture, in this order. The order of MDS specimen collection relative to smear and culture samples was randomized and served as the primary predictor. Outcomes included the normalized copy number of pathogenic RNA detected by MDS, the proportion of MDS samples that were diagnostic, and the agreement of MDS results with cultures. Results: MDS samples from 46 subjects with corneal ulcers were evaluated. MDS was positive in 33 subjects (76%) and had 74% overall agreement with culture results. There was no association between order of MDS sample collection and normalized copy number of genetic material detected ( P = 0.62) or the likelihood of MDS positivity ( P = 0.46). However, the likelihood of agreement between MDS and cultures decreased when MDS corneal swabs were collected after other diagnostic corneal scrapings ( P = 0.05). Conclusions: The overall yield of MDS for detecting the cause of infectious keratitis was not affected by sample collection order. However, diagnostic agreement between MDS and cultures decreased when MDS samples were collected after other specimens. Additional investigation is warranted to determine whether this represents increased sensitivity of MDS compared with cultures or higher susceptibility to contaminants.
    Materialart: Online-Ressource
    ISSN: 0277-3740
    URL: Article
    DOI: 10.1097/ICO.0000000000002766
    RVK:
    XA 37258
    RVK:
    XA 10000
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2022
    ZDB Id: 2045943-9
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 5
    Online-Ressource
    Online-Ressource
    Validation of a Novel Confocal Microscopy Imaging Protocol With Assessment of Reproducibility and Comparison of Nerve Metrics in Dry Eye Disease Compared With Controls
    Ovid Technologies (Wolters Kluwer Health) ; 2021
    In:  Cornea Vol. 40, No. 5 ( 2021-05), p. 603-612
    Details
    In: Cornea, Ovid Technologies (Wolters Kluwer Health), Vol. 40, No. 5 ( 2021-05), p. 603-612
    Kurzfassung: The purposes of this study were to assess the reproducibility of a novel standardized technique for capturing corneal subbasal nerve plexus images with in vivo corneal confocal microscopy and to compare nerve metrics captured with this method in participants with dry eye and control participants. Methods: Cases and controls were recruited based on their International Statistical Classification of Diseases and Related Health Problems (ICD-10) diagnoses. Participants completed the following 3 ocular symptom questionnaires: the Ocular Surface Disease Index, Neuropathic Pain Symptom Inventory, and Dry Eye Questionnaire 5. A novel eye fixation-grid system was used to capture 30 standardized confocal microscopy images of the central cornea. Each participant was imaged twice by different operators. Seven quantitative nerve metrics were analyzed using automated software (ACCmetrics, Manchester, United Kingdom) for all 30 images and a 6-image subset. Results: Forty-seven participants were recruited (25 classified as dry eye and 22 controls). The most reproducible nerve metrics were corneal nerve fiber length [intraclass correlation (ICC) = 0.86], corneal nerve fiber area (ICC = 0.86), and fractal dimension (ICC = 0.90). Although differences were not statistically significant, all mean nerve metrics were lower in those with dry eye compared with controls. Questionnaire scores did not significantly correlate with nerve metrics. Reproducibility of nerve metrics was similar when comparing the entire 30-image montage to a central 6-image subset. Conclusions: A standardized confocal imaging technique coupled with quantitative assessment of corneal nerves produced reproducible corneal nerve metrics even with different operators. No statistically significant differences in in vivo corneal confocal microscopy nerve metrics were observed between participants with dry eye and control participants.
    Materialart: Online-Ressource
    ISSN: 0277-3740
    URL: Article
    DOI: 10.1097/ICO.0000000000002549
    RVK:
    XA 37258
    RVK:
    XA 10000
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2021
    ZDB Id: 2045943-9
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 6
    Online-Ressource
    Online-Ressource
    Synergy Testing of Antiamoebic Agents for Acanthamoeba: Antagonistic Effect of Voriconazole
    Ovid Technologies (Wolters Kluwer Health) ; 2019
    In:  Cornea Vol. 38, No. 10 ( 2019-10), p. 1309-1313
    Details
    In: Cornea, Ovid Technologies (Wolters Kluwer Health), Vol. 38, No. 10 ( 2019-10), p. 1309-1313
    Kurzfassung: To determine whether combinations of commonly used antiamoebic agents display synergy in their ability to kill Acanthamoeba cysts in vitro. Methods: Synergy testing was performed with a microdilution checkerboard assay on 10 clinical Acanthamoeba keratitis isolates collected at the Proctor Foundation from 2008 to 2012. Each isolate was exposed to pairwise combinations of chlorhexidine, propamidine, and voriconazole. The minimum cysticidal concentration (MCC) for each drug pair was estimated for each isolate, and the summed fractional cysticidal concentration (ΣFCC) was calculated for each drug combination in the checkerboard, with synergy defined as a lack of growth at a ΣFCC ≤ 0.5 and antagonism as growth at a ΣFCC 〉 4. Results: Chlorhexidine and propamidine were cysticidal, with median MCCs of 12.5 (range 1.5–50) and 11.7 (range 0.2–250), respectively. Voriconazole was not cysticidal, with a median MCC of 〉 10,000 μg/mL. The combination of chlorhexidine and propamidine did not markedly change the cysticidal activity compared with either drug alone. By contrast, voriconazole antagonized the cysticidal activity of both chlorhexidine and propamidine, with Acanthamoeba growth observed at antagonistic ΣFCCs in 27 of 49 (55.1%, 95% confidence interval 35.7%–78.6%) checkerboard combinations of voriconazole and chlorhexidine and in 58 of 147 (39.5%, 95% confidence interval 14.3%–50.3%) combinations of voriconazole and propamidine. Conclusions: In an in vitro assay, voriconazole reduced the cysticidal activity of 2 commonly used antiamoebic drugs. Although the in vivo drug interactions could be different, these observations may be useful in cases of nonhealing Acanthamoeba keratitis being treated with combination therapies that include voriconazole.
    Materialart: Online-Ressource
    ISSN: 0277-3740
    URL: Article
    DOI: 10.1097/ICO.0000000000002055
    RVK:
    XA 37258
    RVK:
    XA 10000
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2019
    ZDB Id: 2045943-9
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 7
    Online-Ressource
    Online-Ressource
    Antiamoebic Susceptibility in Acanthamoeba Keratitis: Comparison of Isolates From South India and Northern California
    Ovid Technologies (Wolters Kluwer Health) ; 2023
    In:  Cornea Vol. 42, No. 1 ( 2023-01), p. 110-112
    Details
    In: Cornea, Ovid Technologies (Wolters Kluwer Health), Vol. 42, No. 1 ( 2023-01), p. 110-112
    Kurzfassung: Outcomes of Acanthamoeba keratitis are often worse in India than in the United States. The goal of this study was to determine whether antiamoebic susceptibility patterns were different when comparing Acanthamoeba isolates from India with those of the United States. Methods: Acanthamoeba isolates were obtained from corneal scrapings of 43 patients with infectious keratitis seen at the Francis I. Proctor Foundation (N = 23) and Aravind Eye Hospital (N = 20) from 2008 through 2012 and plated on growth media. A previously described minimum cysticidal concentration (MCC) assay was performed by a single laboratory technician to assess susceptibility to 5 antiamoebic agents for all isolates. Testing was conducted in triplicate, with the median MCC chosen for analyses. Results: The MCC (μg/mL) of polyhexamethylene biguanide was 6.25 [IQR 5.47–12.5] for Aravind isolates and 6.25 [IQR 6.25–9.375] for Proctor isolates ( P = 0.75), corresponding values were 6.25 [IQR 3.125–6.25] and 3.125 [IQR 3.125–9.375] for chlorhexidine ( P = 0.81), 2500 [IQR 2500–5000] and 5000 [IQR 1250–20,000] for voriconazole ( P = 0.25), 15.6 [IQR 15.6–39.0625] and 15.6 [IQR 15.6–31.25] for hexamidine ( P = 0.92), and 15.6 [IQR 7.81–15.6] and 15.6 [IQR 7.81–31.25] for propamidine ( P = 0.42). Conclusions: This study found no statistically significant differences in antiamoebic susceptibility of Indian versus US samples from Acanthamoeba keratitis clinical isolates. These findings suggest that differences in antiamoebic susceptibility are likely not responsible for differential outcomes in Acanthamoeba keratitis between the 2 locations.
    Materialart: Online-Ressource
    ISSN: 0277-3740
    URL: Article
    DOI: 10.1097/ICO.0000000000003060
    RVK:
    XA 37258
    RVK:
    XA 10000
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2023
    ZDB Id: 2045943-9
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 8
    Online-Ressource
    Online-Ressource
    Case Series: Unbiased Deep Sequencing Analysis of Acute Infectious Conjunctivitis in an Ambulatory Eye Center in Berkeley, California
    Ovid Technologies (Wolters Kluwer Health) ; 2023
    In:  Optometry and Vision Science Vol. 100, No. 4 ( 2023-4), p. 276-280
    Details
    In: Optometry and Vision Science, Ovid Technologies (Wolters Kluwer Health), Vol. 100, No. 4 ( 2023-4), p. 276-280
    Kurzfassung: Acute infectious conjunctivitis poses significant challenges to eye care providers. It can be highly transmissible, and because etiology is often presumed, correct treatment and management can be difficult. This study uses unbiased deep sequencing to identify causative pathogens of infectious conjunctivitis, potentially allowing for improved approaches to diagnosis and management. PURPOSES This study aimed to identify associated pathogens of acute infectious conjunctivitis in a single ambulatory eye care center. CASE REPORTS This study included patients who presented to the University of California Berkeley eye center with signs and symptoms suggestive of infectious conjunctivitis. From December 2021 to July 2021, samples were collected from seven subjects (ages ranging from 18 to 38). Deep sequencing identified associated pathogens in five of seven samples, including human adenovirus D, Haemophilus influenzae , Chlamydia trachomatis , and human coronavirus 229E. CONCLUSIONS Unbiased deep sequencing identified some unexpected pathogens in subjects with acute infectious conjunctivitis. Human adenovirus D was recovered from only one patient in this series. Although all samples were obtained during the COVID-19 pandemic, only one case of human coronavirus 229E and no SARS-CoV-2 were identified.
    Materialart: Online-Ressource
    ISSN: 1538-9235 , 1040-5488
    URL: Article
    DOI: 10.1097/OPX.0000000000002010
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2023
    ZDB Id: 2083924-8
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 9
    Online-Ressource
    Online-Ressource
    Ocular Clinical Signs and Diagnostic Tests Most Compatible With Keratoconjunctivitis Sicca: A Latent Class Approach
    Ovid Technologies (Wolters Kluwer Health) ; 2020
    In:  Cornea Vol. 39, No. 8 ( 2020-08), p. 1013-1016
    Details
    In: Cornea, Ovid Technologies (Wolters Kluwer Health), Vol. 39, No. 8 ( 2020-08), p. 1013-1016
    Kurzfassung: To evaluate the ocular signs and tests for keratoconjunctivitis sicca (KCS) in the absence of a gold standard. Methods: Cross-sectional study of participants from the Sjögren's International Collaborative Clinical Alliance (SICCA) registry. Participants had oral/ocular/rheumatologic examinations, blood/saliva samples collected, and salivary gland biopsy. Latent class analysis (LCA) identified clusters of patients based on 3 to 4 predictor variables relating to signs or tests of KCS. The resulting model-based “gold standard” classification formed the basis for estimated sensitivity and specificity associated with these predictors. Results: A total of 3514 participants were enrolled into SICCA, with 52.9% classified as SS. LCA revealed a best-fit model with 2 groups. For the gold standard–positive group, an abnormal tear breakup time, ocular staining score (OSS), and Schirmer I had a sensitivity of 99.5%, 91.0%, and 47.4%, respectively. For the gold standard–negative group, an abnormal tear breakup time, OSS, and Schirmer I had a specificity of 32.0%, 84.0%, and 88.5%, respectively. OSS components (fluorescein and lissamine staining), exhibited a sensitivity of 82.6% and 90.5%, respectively, in the gold standard–positive group, whereas these signs in the gold standard–negative group had a specificity of 88.8% and 73.0%, respectively. Conclusions: OSS and its components (fluorescein and lissamine staining) differentiated 2 groups from each other better than other KCS parameters and had relatively high sensitivity and specificity.
    Materialart: Online-Ressource
    ISSN: 0277-3740
    URL: Article
    DOI: 10.1097/ICO.0000000000002311
    RVK:
    XA 37258
    RVK:
    XA 10000
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2020
    ZDB Id: 2045943-9
    Standort Signatur Einschränkungen Verfügbarkeit
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    Online-Ressource
  • 10
    Online-Ressource
    Online-Ressource
    Practice Patterns and Clinician Opinions for Treatment of Ocular Rosacea
    Ovid Technologies (Wolters Kluwer Health) ; 2023
    In:  Cornea Vol. 42, No. 11 ( 2023-11), p. 1349-1354
    Details
    In: Cornea, Ovid Technologies (Wolters Kluwer Health), Vol. 42, No. 11 ( 2023-11), p. 1349-1354
    Kurzfassung: Management of ocular rosacea is challenged by the limited evidence-based systemic treatment guidelines and lack of elucidated mechanisms of treatment efficacy. Methods: We conducted an online survey of clinicians who regularly treat ocular rosacea to elicit their opinions on treatment algorithms and understanding of the treatment's primary mechanism of action. Descriptive statistics and univariate comparisons were reported. Results: One hundred thirty-two participants completed the online survey. Of the 132 respondents, 74% were cornea specialists. Most respondents (85%) favored systemic tetracyclines over macrolides. Providers' specialty training did not significantly influence preference between tetracyclines and macrolides for ocular rosacea management. Among tetracycline prescribers, there was no consensus regarding initial dosage and duration prescribing patterns. Most macrolide prescribers (88%) initiated a 3-week course of 1 gram of azithromycin weekly. Long-term management strategy for treatment-responsive patients varied: 46% preferred to half the initial dose, 29% discontinued pharmacotherapy, and 16% chronically pulse-dosed patients. Most tetracycline prescribers (90%) and macrolide prescribers (73%) postulate their chosen agents' primary mechanism of effect for ocular rosacea is anti-inflammatory. However, there was no consensus in identifying anti-inflammatory doses of either drug class. Furthermore, there is discordance between prescribers’ intended mechanistic effect with the selection of initial dosages for both tetracycline and macrolides for ocular rosacea. Conclusions: Among clinicians who commonly treat ocular rosacea, there is significant community equipoise regarding which dose of tetracycline is best for initial systemic treatment of this disease. In addition, a consensus understanding regarding mechanism of action of this treatment is lacking.
    Materialart: Online-Ressource
    ISSN: 0277-3740
    URL: Article
    DOI: 10.1097/ICO.0000000000003157
    RVK:
    XA 37258
    RVK:
    XA 10000
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2023
    ZDB Id: 2045943-9
    Standort Signatur Einschränkungen Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Online-Ressource
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