In:
Optics Express, Optica Publishing Group, Vol. 30, No. 8 ( 2022-04-11), p. 12545-
Abstract:
Based on synchronous phase shift determination, we propose a differential phase measurement method for differential interference contrast (DIC) microscopy. An on-line phase shift measurement device is used to generate carrier interferograms and determine the phase shift of DIC images. Then the differential phase can be extracted with the least-squares phase-shifting algorithm. In addition to realizing on-line, dynamic, real-time, synchronous and high precision phase shift measurement, the proposed method also can reconstruct the phase of the specimen by using the phase-integral algorithm. The differential phase measurement method reveals obvious advantages in error compensation, anti-interference, and noise suppression. Both simulation analysis and experimental result demonstrate that using the proposed method, the accuracy of phase shift measurement is higher than 0.007 rad. Very accurate phase reconstructions were obtained with both polystyrene microspheres and human vascular endothelial.
Type of Medium:
Online Resource
ISSN:
1094-4087
Language:
English
Publisher:
Optica Publishing Group
Publication Date:
2022
detail.hit.zdb_id:
1491859-6
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