In:
Biomedical Optics Express, Optica Publishing Group, Vol. 12, No. 8 ( 2021-08-01), p. 5139-
Abstract:
A technique using Linnik-based optical coherence microscopy (OCM), with built-in fluorescence microscopy (FM), is demonstrated here to describe cellular-level morphology for fresh porcine and biobank tissue specimens. The proposed method utilizes color-coding to generate digital pseudo-H & E (p-H & E) images. Using the same camera, colocalized FM images are merged with corresponding morphological OCM images using a 24-bit RGB composition process to generate position-matched p-H & E images. From receipt of dissected fresh tissue piece to generation of stitched images, the total processing time is 〈 15 min for a 1-cm 2 specimen, which is on average two times faster than frozen-section H & E process for fatty or water-rich fresh tissue specimens. This technique was successfully used to scan human and animal fresh tissue pieces, demonstrating its applicability for both biobank and veterinary purposes. We provide an in-depth comparison between p-H & E and human frozen-section H & E images acquired from the same metastatic sentinel lymph node slice (∼10 µm thick), and show the differences, like elastic fibers of a tiny blood vessel and cytoplasm of tumor cells. This optical sectioning technique provides histopathologists with a convenient assessment method that outputs large-field H & E-like images of fresh tissue pieces without requiring any physical embedment.
Type of Medium:
Online Resource
ISSN:
2156-7085
,
2156-7085
Language:
English
Publisher:
Optica Publishing Group
Publication Date:
2021
detail.hit.zdb_id:
2572216-5
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