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  • Wiley-Blackwell  (23)
  • Nature Publishing Group (NPG)  (18)
  • 1
    Digitale Medien
    Digitale Medien
    Species-specificity of the cohesin-dockerin interaction between Clostridium thermocellum and Clostridium cellulolyticum: Prediction of specificity determinants of the dockerin domain (1997)
    New York, NY : Wiley-Blackwell
    Proteins: Structure, Function, and Genetics 29 (1997), S. 517-527 
    Details
    ISSN: 0887-3585
    Schlagwort(e): cellulosome ; cellulases ; cohesin domain ; scaffoldin subunit ; EF-hand motif ; molecular modeling ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: The cross-species specificity of the cohesin-dockerin interaction, which defines the incorporation of the enzymatic subunits into the cellulosome complex, has been investigated. Cohesin-containing segments from the cellulosomes of two different species, Clostridium thermocellum and Clostridium cellulolyticum, were allowed to interact with cellulosomal (dockerin-containing) enzymes from each species. In both cases, the cohesin domain of one bacterium interacted with enzymes from its own cellulosome in a calcium-dependent manner, but the same cohesin failed to recognize enzymes from the other species. Thus, in the case of these two bacteria, the cohesin-dockerin interaction seems to be species-specific. Based on intra- and cross-species sequence comparisons among the different dockerins together with their known specificities, we tender a prediction as to the amino-acid residues critical to recognition of the cohesins. The suspected residues were narrowed down to only four, which comprise a repeated pair located within the calcium-binding motif of two duplicated sequences, characteristic of the dockerin domain. According to the proposed model, these four residues do not participate in the binding of calcium per se; instead, they appear to serve as recognition codes in promoting interaction with the cohesin surface. Proteins 29:517-527, 1997. © 1997 Wiley-Liss, Inc.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
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  • 2
    Digitale Medien
    Digitale Medien
    Thermogravimetric analysis of polystyrene: Influence of sample weight and heating rate on thermal and kinetic parameters (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 61 (1996), S. 187-197 
    Details
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: This article studies the influence of the heating rate and sample weight on the thermal decomposition of polystyrene (first-order kinetics). For this purpose, the kinetic parameters (i.e., frequency factor and activation energy), variables at the maximum decomposition rate (such as conversion, reaction rate, and temperature), as well as some characteristic temperatures have been determined for a series of experiments where the heating rate varies (0.5-11.5 K/min) and also, the sample weight (6.0-25.1 mg). Some mathematical equations have been developed that allow: (1) evaluation of the activation energy of thermal decomposition by different ways and comparing the results obtained; (2) relating different parameters between themselves, such as the heating rate with the temperature at the maximum decomposition rate or the frequency factor with the heating rate and sample weight. Finally, some theoretical explanations of the variation of thermal and kinetic parameters have been proposed. © 1996 John Wiley & Sons, Inc.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
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  • 3
    Digitale Medien
    Digitale Medien
    Effect of the crosslinking degree on curing kinetics of an epoxy-anhydride systemThis article was presented during the 6th European Symposium on Thermal Analysis and Calorimetry held in Grado, Italy, September 11-16, 1994. (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 56 (1995), S. 1413-1421 
    Details
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: The cure kinetics of a diglycidyl ether of bisphenol A (DGEBA)-based epoxy resin with methyltetrahydrophthalic anhydride and an accelerator was studied by nonisothermal DSC data. The systems were uncured resin and partially cured with the following extents of cure measured by the residual heat method (αDSC): 0.37, 0.63, 0.81, and 0.90. The activation energy calculated by the Kissinger method increases from 63 kJ/mol for the uncured epoxy to 77 kJ/mol for the partially cured with αDSC = 0.90. Additionally, the activation energy calculated by the isoconversional method shows a dependence on the conversion degree α. The activation energy tends to decreases initially with the conversion degree, possibly due to the autocatalytic effect; then, it passes through a minimum about α = 0.4 and, finally, increases slightly due to the increase of crosslinks which reduce the mobility of the unreacted groups. A simple, consistent method of kinetic analysis was applied. This method enables one to select the most convenient model and the calculation of kinetic parameters. A two-parameter (m, n) autocatalytic model (S̆esták-Berggren equation) was found to be the most convenient model to study the curing of epoxy systems. The results show a dependence of the kinetic parameters on the initial degree of crosslinking of the partially cured epoxy. The exponent m tends to decrease with the extent of cure, while the exponent n remains practically invariable. These results show a change of the kinetic when the initial extent of cure of the epoxy system increases. The In A data, A being the preexponenetial factor in the Arrhenius dependence of the temperature on the rate of conversion, increase with the extent of cure, showing a correlation with the calculated activation energy values. The nonisothermal DSC curves theoretically calculated show a very good agreement with the experimental data. The two-parameter (m, n) autocatalytic model gives a good description of the curing kinetics of epoxy resins with different extents of cure. © 1995 John Wiley & Sons, Inc.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/app.1995.070561104
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  • 4
    Digitale Medien
    Digitale Medien
    FTIR and DSC study of HDPE structural changes and mechanical properties variation when exposed to weathering aging during canadian winter (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 60 (1996), S. 153-159 
    Details
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: The aging of high-density polyethylene (HDPE) when exposed to drastic climatic conditions (Canadian winter, characterized by low temperature and abrupt temperature variations between the night and the day) was studied. The importance of degradation was determined by evaluating the microstructural changes in HDPE (i.e., oxidations, ramifications, and polymeric chain breaking) by means of FTIR spectrophotometry. The crystallinity variation in HDPE by FTIR and DSC was also studied. Both techniques led to coherent results: there was a loss of crystallinity due to weathering degradation. This crystallinity reduction produced a drastic decrease in impact energy. However, the other properties evaluated were not significantly affected. © 1996 John Wiley & Sons, Inc.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
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  • 5
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    Efficacy and safety of biologics in erythrodermic psoriasis: a multicentre, retrospective study (2012)
    Wiley-Blackwell
    Details
    Publikationsdatum: 2012-04-15
    Beschreibung: Background:  Even though efficacy of biologics has been extensively studied in psoriasis vulgaris, studies in erythrodermic psoriasis, the most severe form of the disease, have beenscarcelyreported. Objectives:  To address the efficacy and safety of biologics in patients with erythrodermic psoriasis. Methods:  A multicentre national retrospective study was performed using the French Psoriasis Group network. Patients showing psoriasis involving at least 90% of body surface area (BSA), and in whom severity of the disease had been evaluated before and after 3 and/or 6 months of treatment with biologics, were enrolled in the study. Results were expressed using intention to treat analysis. Results:  We included 28 patients, representing 42 flares of erythrodermic psoriasis treated with infliximab (n=24), adalimumab (7), etanercept (6), ustekinumab (3) or efalizumab (2). A 75% improvement of BSA or PASI index 12 to 14 weeks after treatment onset was reached in 48% of flares treated with infliximab, in 50% of those treated with adalimumab and in 40% of those treated with etanercept. Twelve serious adverse events consisting of bacterial infection in 7 of them were observed. Biological treatment was discontinued for safety concern in 19% of cases. A given biologic was pursued up to 48 weeks in 34% of flares. Conclusion:  Biologics show overall good short-term efficacy, but treatment switch due to lack of efficacy or side effects is frequently observed on a longer term, with one third of patients still receiving the drug after one year. The most significant safety concern consists of severe infections.
    Print ISSN: 0007-0963
    Digitale ISSN: 1365-2133
    Thema: Medizin
    Publiziert von Wiley-Blackwell
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  • 6
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    TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells (2013)
    Nature Publishing Group (NPG)
    Details
    Publikationsdatum: 2013-07-02
    Beschreibung: Nature Cell Biology 15, 818 (2013). doi:10.1038/ncb2774 Authors: Annamaria Biroccio, Julien Cherfils-Vicini, Adeline Augereau, Sébastien Pinte, Serge Bauwens, Jing Ye, Thomas Simonet, Béatrice Horard, Karine Jamet, Ludovic Cervera, Aaron Mendez-Bermudez, Delphine Poncet, Renée Grataroli, Claire T’kint de Rodenbeeke, Erica Salvati, Angela Rizzo, Pasquale Zizza, Michelle Ricoul, Céline Cognet, Thomas Kuilman, Helene Duret, Florian Lépinasse, Jacqueline Marvel, Els Verhoeyen, François-Loïc Cosset, Daniel Peeper, Mark J. Smyth, Arturo Londoño-Vallejo, Laure Sabatier, Vincent Picco, Gilles Pages, Jean-Yves Scoazec, Antonella Stoppacciaro, Carlo Leonetti, Eric Vivier & Eric Gilson
    Print ISSN: 1465-7392
    Digitale ISSN: 1476-4679
    Thema: Biologie , Medizin
    Publiziert von Nature Publishing Group (NPG)
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  • 7
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    The relationship between heterotrophic feeding and inorganic nutrient availability in the scleractinian coral T. reniformis under a short-term temperature increase (2015)
    Wiley-Blackwell
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    Publikationsdatum: 2015-10-01
    Beschreibung: Worldwide increase in seawater temperature represents one of the major threats affecting corals, which experience bleaching, and thereafter a significant decrease in photosynthesis and calcification. The impact of bleaching on coral physiology may be exacerbated when coupled with eutrophication, i.e., increasing plankton, inorganic nutrient concentrations, sedimentation and turbidity due to coastal urbanization. Whereas zooplankton provision (heterotrophy) may alleviate the negative consequences of thermal stress, inorganic nutrient supply may exacerbate them, which creates a paradox. Our experimental study aims to disentangle the effects of these two components of eutrophication on the physiological response of Turbinaria reniformis subject to normal and to a short-term temperature increase. Additionally, three different inorganic nutrient ratios were tested to assess the influence of nutrient stoichiometry on coral physiology: control (ambient SW 0.5 μ M N and 0.1 μ M P), N only (ambient + 2 μ M N) and N + P (ambient + 2 μ M N and + 0.5 μ M P). Our results show a deleterious effect of a 2 μ M nitrate enrichment alone (N) on coral photosynthetic processes under thermal stress as well as on calcification rates when associated with heterotrophy. On the contrary, a coupled nitrate and phosphorus enrichment (N + P) maintained coral metabolism and calcification during thermal stress and enhanced them when combined with heterotrophy. Broadly, our results shed light on the tight relationship existing between inorganic nutrient availability and heterotrophy. Moreover, it assesses the relevance of N: P stoichiometry as a determining factor for the health of the holobiont that may be adapted to specific nutrient ratios in its surrounding environment.
    Print ISSN: 0024-3590
    Digitale ISSN: 1939-5590
    Thema: Biologie , Geologie und Paläontologie , Physik
    Publiziert von Wiley-Blackwell im Namen von The Association for the Sciences of Limnology and Oceanography (ASLO).
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  • 8
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    Hyperpolarized [1,13C]pyruvate in lysed human erythrocytes: effects of co-substrate supply on reaction time courses (2014)
    Wiley-Blackwell
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    Publikationsdatum: 2014-08-12
    Beschreibung: Hyperpolarized [1, 13 C]pyruvate was injected rapidly into haemolysates in which hydrolysis of nicotinamide adenine dinucleotide (phosphate) (NAD(P))/NAD(P)H had been inhibited with nicotinamide. Haemolysates provide a stable glycolytic system in which membrane permeability is not a flux-controlling step, and they enable the concentration of NADH to be adjusted experimentally while keeping the rest of the sample with the same composition as that of the cytoplasm of the cell (albeit diluted twofold at the time of injection of the [1, 13 C]pyruvate). We showed that the maximum amplitude of the 13 C NMR signal from the [1, 13 C] L -lactate, produced from [1, 13 C]pyruvate, and the time at which it occurred was dependent on NADH concentration, as predicted by enzyme-kinetic analysis. The main feature of such curves was dictated by the immediacy of the supply of the co-substrate of lactate dehydrogenase (LDH, EC 1.1.1.27), and we posit that this also pertains in vivo in various tissues including neoplasms. By constructing an appropriate mathematical model and by using a Markov-chain Monte Carlo approach, we fitted experimental data to estimate LDH and NADH concentrations. Experiments carried out with only endogenous NADH present enabled the estimation of its effective concentration in human RBCs; the ability to make this estimate is a special feature of the rapid-dissolution dynamic nuclear polarization method. We found an endogenous NADH concentration in human RBCs two to four times higher than previously reported. Copyright © 2014 John Wiley & Sons, Ltd. We measured lactate dehydrogenase and NADH concentrations in human erythrocytes by using 13 C NMR rapid-dissolution dynamic nuclear polarization. A detailed enzyme kinetic model was used in the description of the experimental system, and a Markov-chain Monte Carlo procedure yielded estimates of parameter values. We report new estimates of concentrations of LDH and NADH in human erythrocytes; the former was about three times lower than previously reported while the latter was about three times higher.
    Print ISSN: 0952-3480
    Digitale ISSN: 1099-1492
    Thema: Medizin
    Publiziert von Wiley-Blackwell
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  • 9
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    Vertical and Oblique ionospheric soundings over the long haul HF link between Antarctica and Spain (2015)
    Wiley-Blackwell
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    Publikationsdatum: 2015-08-28
    Beschreibung: This paper presents a comparative study between the oblique sounding results, the ITU Rec533 HF prediction model and the vertical sounding results of a transequatorial long haul link. The long haul link is a 12760km link between the Spanish Antarctic Station, SAS, located in the Livingston Island and the Ebro Observatory (OE) in Spain. The data was collected during three consecutive surveys (2009/10, 2010/11 and 2011/12). The ionospheric channel from the SAS to the OE is studied in terms of frequency availability as function of time using the measurements of an oblique incidence sounder (OIS) and measurements of several vertical incidence sounding stations (VIS) placed near the estimated radiopropagation path. The results obtained show promising correlations between VIS and OIS measurements, and led us think that the frequency of largest availability for this particular long haul radio link can be estimated from the VIS sounding measurements.
    Print ISSN: 0048-6604
    Digitale ISSN: 1944-799X
    Thema: Geologie und Paläontologie , Physik
    Publiziert von Wiley-Blackwell im Namen von American Geophysical Union (AGU).
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  • 10
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    Thermally tolerant corals have limited capacity to acclimatize to future warming (2014)
    Wiley-Blackwell
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    Publikationsdatum: 2014-03-12
    Beschreibung: Thermal stress affects organism performance differently depending on the ambient temperature to which they are acclimatized, which varies along latitudinal gradients. This study investigated whether differences in physiological responses to temperature are consistent with regional differences in temperature regimes for the stony coral Oculina patagonica . To resolve this question we experimentally assessed how colonies originating from four different locations characterized by 〉3°C variation in mean maximum annual temperature responded to warming from 20 to 32°C. We assessed plasticity in symbiont identity, density, and photosynthetic properties, together with changes in host tissue biomass. Results show that, without changes in the type of symbiont hosted by coral colonies, O. patagonica has limited capacity to acclimatize to future warming. We found little evidence of variation in overall thermal tolerance, or in thermal optima, in response to spatial variation in ambient temperature. Given that the invader O. patagonica is a relatively new member of the Mediterranean coral fauna our results also suggest that coral populations may need to remain isolated for a long period of time for thermal adaptation to potentially take place. Our study indicates that for O. patagonica , mortality associated with thermal stress manifests primarily through tissue breakdown under moderate but prolonged warming (which does not impair symbiont photosynthesis and, therefore, does not lead to bleaching). Consequently, projected global warming is likely to causes repeat incidents of partial and whole colony mortality and might drive a gradual range contraction of Mediterranean corals. This article is protected by copyright. All rights reserved.
    Print ISSN: 1354-1013
    Digitale ISSN: 1365-2486
    Thema: Biologie , Energietechnik , Geographie
    Publiziert von Wiley-Blackwell
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