Keywords:
High performance liquid chromatography.
;
Biomolecules -- Separation.
;
Electronic books.
Description / Table of Contents:
Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.
Type of Medium:
Online Resource
Pages:
1 online resource (791 pages)
Edition:
2nd ed.
ISBN:
9780203911594
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=216201
Language:
English
Note:
Intro -- Preface -- Contents -- Contributors -- Silica as a Support in HPLC of Biomacromolecules -- Organic Polymer Support Materials -- Size Exctusion Chromatography -- Ion-Exchange Chromatography -- Reversed-Phase and Hydrophobic Interaction Chromatography of Proteins and Peptides -- Metal Interaction Chromatography -- Preparative Chromatography -- Sample Preparation -- Gradient Elution Separation of Large Biomolecules -- Anaiyticai HPLC of Peptides -- HPLC of Membrane Proteins -- HPLC of Cereat Endosperm Storage Proteins -- Anatysis of Hemogtobin Variants and HbA1c -- Antibodies -- Gtycoproteins -- tmmunodetection of Proteins in High-Reso!ution Separation Systems -- Characterization of Proteins, Peptides, and Po!ynuc!eotides by Mass Spectrometry -- Detection and Anatysis of Proteins by High- Performance Liquid Chromatography with Photodiode Array Detection -- index.
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