In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 63, No. Pt_1 ( 2013-01-01), p. 212-218
Abstract:
A novel actinomycete, strain A4036 T , was isolated from a soil sample collected from the Jabi district in Abuja, Nigeria. The taxonomic position of strain A4036 T was established using a combination of genotypic and phenotypic analyses. The organism formed extensively branched substrate and aerial hyphae that generated spiral chains of spores with warty surfaces. The cell wall contained meso -diaminopimelic acid and the cell-wall sugars were glucose, madurose, mannose and ribose. The predominant menaquinone was MK-9(H 4 ). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylmethylethanolamine, phosphatidylinositol mannoside, hydroxy-phosphatidylethanolamine, hydroxy-phosphatidylmethylethanolamine, two unidentified phospholipids and four unknown glucosamine-containing phospholipids. The major cellular fatty acids were iso-C 16 : 0 2-OH, iso-C 16 : 0 and 10-methyl C 17 : 0 . On the basis of 16S rRNA gene sequence similarity studies, strain A4036 T grouped in the genus Nonomuraea , being most closely related to Nonomuraea angiospora IFO 13155 T (99.05 %), Nonomuraea candida HMC10 T (98.78 %), Nonomuraea kuesteri GW 14-1925 T (98.49 %), Nonomuraea endophytica YIM 65601 T (98.42 %), Nonomuraea maheshkhaliensis 16-5-14 T (98.40 %), Nonomuraea turkmeniaca DSM 43926 T (98.38 %), Nonomuraea helvata IFO 14681 T (98.29 %), Nonomuraea rubra DSM 43768 T (98.10 %) and Nonomuraea salmonea DSM 43678 T (98.06 %). Levels of 16S rRNA gene sequence similarity to the type strains of other species of the genus Nonomuraea were 〈 98 %. Despite the high 16S rRNA gene sequence similarities, DNA–DNA relatedness values and phenotypic data demonstrated that strain A4036 T was clearly distinguished from all closely related species of the genus Nonomuraea . Thus, this isolate is considered to represent a novel species of the genus Nonomuraea , for which the name Nonomuraea jabiensis sp. nov. is proposed. The type strain is A4036 T ( = DSM 45507 T = KCTC 19870 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.039362-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2013
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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