In:
Journal of Medical Microbiology, Microbiology Society, Vol. 58, No. 7 ( 2009-07-01), p. 945-950
Abstract:
Clinical isolates of Mycobacterium avium ( n =81) from patients with pulmonary infections who were HIV-negative and isolates ( n =33) from HIV-positive patients were subjected to genetic analysis by PCR detection of three M. avium -specific insertion sequences (IS 901 , IS 1245 and IS 1311 ), and nucleotide sequencing of the heat-shock protein 65 ( hsp65 ) gene. All clinical isolates were identified as ‘ M. avium subspecies hominissuis ’ by sequence analysis of hsp65 . Compared with clinical isolates of M. avium reported elsewhere, IS 1245 was found less frequently in Japanese isolates (96/114 isolates, 84 %) and IS 901 was detected more frequently (76/114 isolates, 67 %). One isolate was found to lack IS 1311 , which has not been reported previously for ‘ M. avium subsp. hominissuis ’. Nucleotide sequence analysis of the PCR products for IS 901 revealed that all clinical isolates had the same new insertion sequence, designated IS Mav6 , which had 60 point mutations compared with the nucleotide sequence of the original IS 901 . These results suggest that ‘ M. avium subsp. hominissuis ’ with IS Mav6 is prevalent in Japan. IS Mav6 may have implications for the virulence of M. avium and contribute to an increase of M. avium infections in this country.
Type of Medium:
Online Resource
ISSN:
0022-2615
,
1473-5644
DOI:
10.1099/jmm.0.008623-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2009
detail.hit.zdb_id:
2083944-3
SSG:
12
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