In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 72, No. 4 ( 2022-04-28)
Abstract:
Strain NGK65 T , a novel hexadecane degrading, non-motile, Gram-positive, rod-to-coccus shaped, aerobic bacterium, was isolated from plastic polluted soil sampled at a landfill. Strain NGK65 T hydrolysed casein, gelatin, urea and was catalase-positive. It optimally grew at 28 °C, in 0–1% NaCl and at pH 7.5–8.0. Glycerol, d -glucose, arbutin, aesculin, salicin, potassium 5-ketogluconate, sucrose, acetate, pyruvate and hexadecane were used as sole carbon sources. The predominant membrane fatty acids were iso-C 16:0 followed by iso-C 17:0 and C 18:1 ω 9 c . The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and hydroxyphosphatidylinositol. The cell-wall peptidoglycan type was A3γ, with ll -diaminopimelic acid and glycine as the diagnostic amino acids. MK 8 (H 4 ) was the predominant menaquinone. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NGK65 T belongs to the genus Nocardioides (phylum Actinobacteria ), appearing most closely related to Nocardioides daejeonensis MJ31 T (98.6%) and Nocardioides dubius KSL-104 T (98.3%). The genomic DNA G+C content of strain NGK65 T was 68.2%. Strain NGK65 T and the type strains of species involved in the analysis had average nucleotide identity values of 78.3–71.9% as well as digital DNA–DNA hybridization values between 22.5 and 19.7%, which clearly indicated that the isolate represents a novel species within the genus Nocardioides . Based on phenotypic and molecular characterization, strain NGK65 T can clearly be differentiated from its phylogenetic neighbours to establish a novel species, for which the name Nocardioides alcanivorans sp. nov. is proposed. The type strain is NGK65 T (=DSM 113112 T =NCCB 100846 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.005319
Language:
English
Publisher:
Microbiology Society
Publication Date:
2022
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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