In:
Journal of Medical Microbiology, Microbiology Society, Vol. 59, No. 5 ( 2010-05-01), p. 573-579
Abstract:
In this study, we investigated the anti-inflammatory and reinforcing barrier effects of Lactobacillus casei subsp. rhamnosus (Lcr35) on Caco-2 intestinal epithelial cells already exposed to Salmonella LPS. Using the Transwell co-culture model, Salmonella LPS was apically added to polarized Caco-2 cells co-cultured with peripheral blood mononuclear cells (PBMCs) in the basolateral compartment. LPS-stimulated Caco-2 cells were incubated with Lcr35 for 1, 6, 24 or 48 h. Apical inoculation of Lcr35 after 48 h significantly inhibited the basolateral secretion of interleukin-8 (IL-8) in the Caco-2/PBMC co-culture. The PCR analysis showed that Lcr35 significantly downregulated mRNA expression of monocyte chemoattractant protein 1 (MCP-1) ( P 〈 0.05) and had a trend of decreasing mRNA expression of IL-8 ( P =0.05), but did not alter mRNA expression of transforming growth factor- β 1 in LPS-stimulated Caco-2 cells at 48 h after addition of Lcr35. Compared to non-LPS-pretreated controls, transepithelial electrical resistance (TEER) of the polarized Caco-2 cell monolayers pretreated with LPS for 48 h was decreased by 9.9 % ( P 〈 0.05). Additionally, compared to those cells only treated with LPS, apical co-incubation with Lcr35 showed biphasic TEER levels increased by 12.1 % ( P 〈 0.001), 5.7 % ( P 〈 0.05) and 86.8 % ( P 〈 0.001) in the Caco-2 cell monolayers compared to those without Lcr35 treatment after 1, 6 and 48 h, respectively. In conclusion, Lcr35 can exert anti-inflammatory effects and ameliorate barrier dysfunction in the Salmonella LPS-pretreated inflamed intestinal epithelium in vitro .
Type of Medium:
Online Resource
ISSN:
0022-2615
,
1473-5644
DOI:
10.1099/jmm.0.009662-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2010
detail.hit.zdb_id:
2083944-3
SSG:
12
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