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  • 1
    In: Medycyna Weterynaryjna, Medycyna Weterynaryjna - Redakcja, Vol. 76, No. 07 ( 2020), p. 6422-2020
    Abstract: The aim of this study was to determine the influence of the ageing period on the physicochemical properties and lipid quality indicators of pig meat from the native Puławska breed. The experiment was carried out on samples of skeletal muscles (adjacent part of M. longissimus thoracis and M. longissimus lumborum – LTL, and M. semimembranosus – SM) collected 24 h after slaughter from 30 carcasses of Puławska castrated male pigs. Following collection, samples were packaged in vacuum and stored for up to 14 day at a temperature of 4°C (± 1°C). The meat pH, colour (CIE L*a*b*), water-holding capacity (drip loss – DL and cooking loss – CL), shear force (WBSF), energy force, and lipid quality indicators (free fatty acids – FFA, peroxide value – PV, and thiobarbituric acid reactive substances expressed as malondialdehyde MDA – TBARS) were measured. A typical course of postmortem glycogenolysis for normal meat was demonstrated. The pH of muscles 45 min after slaughter was 6.55 for LTL and 6.61 for SM, and after 24 h it was 5.62 and 5.66, respectively. As ageing progressed, the DL of the meat increased significantly (on average 2.4 times), while CL were not significantly changed, being 24.83% on average. WBSF decreased by approximately 40% for LTL, and by 18% for SM. The WBSF value adopted for tender meat ( 〈 45 N/cm2) was obtained on day 14 of the experiment. The progressive oxidative changes in intramuscular fat were not advanced, averaging from 2.06% for FFA, 0.62 meq O2/kg for PV and 0.24 MDA/kg for TBARS on day 4 post mortem to 3.71%, 1.82 meq O2/kg and 0.48 mg MDA/kg, respectively, on day 14 post mortem. Ageing of pork under vacuum positively influenced the quality characteristics of the meat, with relatively minor oxidative changes. An extended ageing period of up to 14 days can measurably improve the quality characteristics of fresh meat obtained from Puławska breed pigs, especially for culinary purposes.
    Type of Medium: Online Resource
    ISSN: 0025-8628
    Language: Unknown
    Publisher: Medycyna Weterynaryjna - Redakcja
    Publication Date: 2020
    detail.hit.zdb_id: 2777172-6
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  • 2
    Online Resource
    Online Resource
    Medycyna Weterynaryjna - Redakcja ; 2019
    In:  Medycyna Weterynaryjna Vol. 75, No. 02 ( 2019), p. 6204-2019
    In: Medycyna Weterynaryjna, Medycyna Weterynaryjna - Redakcja, Vol. 75, No. 02 ( 2019), p. 6204-2019
    Abstract: The aim was to assess the quality and safety of traditional smoked meats produced from the meat of Puławska breed pigs. The research material consisted of two skeletal muscles, namely, semimembranosus and longissimus dorsi, taken from 10 carcasses of Puławska breed fatteners. Smoked meats were produced with a 3% addition of salt, spices and acid milk whey (2%). The products were assessed in terms of their basic chemical components (water, protein, fat and salt), colour parameters in the CIE L*a*b* colour space and the content of contaminants, i.e. PAHs [chrysen, benzo(a)anthracene, benzo(b)fluoranthene, benzo(a)pyrene] and residues of nitrates (III) and (IV). The muscle type had no significant effect on the chemical composition of the products, although a higher content of fat and a lower content of salt were found in loin (by 2.79 and 0.38 percentage points, respectively). Smoked meat from longissimus dorsi was significantly lighter in colour (L*; 60.85 vs. 69.28), whereas smoked meats from ham showed a significantly greater degree of redness (a*; 13.24 vs. 8.75). The degree of yellowness of both products was similar (b*; 8.56 vs. 8.85). The content of 4 PHAs and B(a)P in smoked meats from ham was below the permissible limits for traditional products, which are 30 μg/kg and 5 μg/kg, respectively. In the case of smoked meats from loin, the threshold value (30 μg/kg) for the total of 4 PHAs was exceeded in two products, which affected their relatively high average content in smoked meat from this muscle (48.28 μg/kg). Nitrates III and IV were present only in one smoked meat from ham at the levels of 12 ± 2.7 mg/kg (expressed as NaNO2) and 48 ± 11 mg/kg (expressed as NaNO3), respectively. For the other products (from both ham and loin), the results were below the detection limit (results not presented in the table). From the nutritional point of view, the chemical composition of these smoked meats was beneficial: the protein content was high, the fat content was optimal, the content of table salt did not exceed the permissible limit, and there were only trace quantities of nitrates III and V. As far as PHAs are concerned, some measures should be taken to reduce their level, especially in smoked meats from loin.
    Type of Medium: Online Resource
    ISSN: 0025-8628
    Language: Unknown
    Publisher: Medycyna Weterynaryjna - Redakcja
    Publication Date: 2019
    detail.hit.zdb_id: 2777172-6
    SSG: 22
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  • 3
    Online Resource
    Online Resource
    Medycyna Weterynaryjna - Redakcja ; 2019
    In:  Medycyna Weterynaryjna Vol. 75, No. 05 ( 2019), p. 6258-2019
    In: Medycyna Weterynaryjna, Medycyna Weterynaryjna - Redakcja, Vol. 75, No. 05 ( 2019), p. 6258-2019
    Abstract: Oligodendrocytes (OLs) are myelinating cells of the central nervous system (CNS). They are a highly specialized type of glial cell in the CNS of vertebrates, which guarantee the transmission of action potentials over long distances by producing a myelin sheath that wraps adjacent axons. Although they are often credited merely with participation in myelination, recent research has led to a radical change in the understanding the role of these glial cells. OLs are currently understood to be plastic and adaptive cells, capable of responding quickly to changes taking place in the spatial neuronal network in the CNS. Due to their complex differentiation process and their physiology, OLs are among the most sensitive cells in the CNS. Finding answers about their interactions with other types of glial cells may result in benefits in the form of neuroprotection and axon plasticity. Damage to OLs and the myelin sheath is one of processes contributing to the development of crippling neurological diseases, although the role of these cells in neurodegeneration remains controversial. This article not only presents OLs as cells whose ultimate goal is to produce myelin sheaths, but also discusses their involvement in neurodegenerative diseases.
    Type of Medium: Online Resource
    ISSN: 0025-8628
    Language: Unknown
    Publisher: Medycyna Weterynaryjna - Redakcja
    Publication Date: 2019
    detail.hit.zdb_id: 2777172-6
    SSG: 22
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  • 4
    Online Resource
    Online Resource
    Medycyna Weterynaryjna - Redakcja ; 2020
    In:  Medycyna Weterynaryjna Vol. 76, No. 03 ( 2020), p. 6379-2020
    In: Medycyna Weterynaryjna, Medycyna Weterynaryjna - Redakcja, Vol. 76, No. 03 ( 2020), p. 6379-2020
    Abstract: The aim of the study was to assess the endogenic production of vitamin C contained in the sow’s colostrum and milk and its influence on piglet rearing parameters. The study was conducted on 60 Zlotnicka White sows and 642 piglets from their litters (366 male and 276 female piglets). The sows were in their 3rd, 4th, 6th or 7th lactation. There were 15 sows in each lactation group. The study covered a period of one lactation. The feed of the sows was not supplemented with vitamin C. During the experiment the piglets received only their mothers’ milk. The piglets were weighed on the 1st, 7th and 21st day of life (kg). Their mortality between the 1st and 7th day of life and between the 8th and 21st day of life (%) was recorded. The daily growth (g) was measured on each day when the piglets were weighed. Samples of colostrum and milk were collected manually from all active mammary glands on the 1st, 7th and 21st day of lactation. On each day of the experiment, two samples of about 7 ml were collected from each sow. A total of 360 samples were analysed (60 sows × 3 days × 2 samples). In order to verify the influence of vitamin C on piglet rearing, two concentration levels of vitamin C were distinguished according to its content in colostrum and milk (Tab. 1). The research showed that the content of vitamin C varied during lactation. The highest mean concentration of vitamin C, i.e. 1.14 mg/100 ml, was noted on the 1st day. On the 21st day it decreased to 0.65 mg/100 ml. During 21 days of lactation the content of vitamin C decreased by 43%. Sows in their fourth lactation produced colostrum with the highest content of vitamin C, i.e. 1.29 mg/100 ml. Animals that lactated for the sixth time had the highest content of vitamin C in their milk, i.e. 0.75 and 0.70 mg/100 ml, on the 7th and 21st day, respectively. The daily growth of piglets was greater if the content of vitamin C in colostrum and milk collected during rearing was high (level II). On average, the differences amounted to about 68 g. The higher the content of vitamin C in the secretion from the sows’ mammary glands was, the lower the piglet mortality was. Statistically significant (p ≤ 0.01) relationships were found between daily growth and the content of vitamin C. The higher concentration of vitamin C produced better results in rearing in the form of a faster growth and lower mortality of piglets.
    Type of Medium: Online Resource
    ISSN: 0025-8628
    Language: Unknown
    Publisher: Medycyna Weterynaryjna - Redakcja
    Publication Date: 2020
    detail.hit.zdb_id: 2777172-6
    SSG: 22
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  • 5
    Online Resource
    Online Resource
    Medycyna Weterynaryjna - Redakcja ; 2022
    In:  Medycyna Weterynaryjna Vol. 77, No. 12 ( 2022), p. 6726-2022
    In: Medycyna Weterynaryjna, Medycyna Weterynaryjna - Redakcja, Vol. 77, No. 12 ( 2022), p. 6726-2022
    Abstract: The aim of the research was to evaluate the genetic structure of Ukrainian Holstein cattle (n = 150) of the state enterprise Chayka (Kiev region, Ukraine) on the basis of the analysis of 12 microsatellite DNA loci and 3 QTL genes: k-casein (CSN3), prolactin (PRL), and fatty acid synthase (FASN). A total of 93 allelic variants were identified, and the largest number of alleles were found for TGLA53, TGLA122 and TGLA227 loci, the mean PIC = 0.693, and the heterozygosity index for all loci was 0.7314. The greatest number of effective alleles per locus were found for the BM1824 locus. Based on association analyses, complex genotypes were established for the three genes (CSN3, PRL, FASN). It was found that the AAPRL AACSN3 AAFASN genotype determined the highest milk yield (8351 kg / lactation) and the protein content in milk (3.10%), whereas the AAPRL ABCSN3 GGFASN genotype determined the highest level of fat content in milk (3.80%).
    Type of Medium: Online Resource
    ISSN: 0025-8628
    Language: Unknown
    Publisher: Medycyna Weterynaryjna - Redakcja
    Publication Date: 2022
    detail.hit.zdb_id: 2777172-6
    SSG: 22
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  • 6
    In: Medycyna Weterynaryjna, Medycyna Weterynaryjna - Redakcja, Vol. 73, No. 7 ( 2017), p. 395-398
    Abstract: The paper presents the genetic characteristics of Pulawska breed pigs carried out on the basis of cytogenetic and molecular studies. Among chromosome markers, polymorphic variants of specific chromosome structures such as centromeric heterochromatin areas (C bands) and nucleolar organizing regions (NOR bands) were taken into account. The described breed-specific tendencies concerning size polymorphism of these structures are the source of chromosome markers that are useful for identifying the linkage with genes controlling important production traits. On the other hand, molecular studies presented in this work included DNA markers of the STR and SNP type (with particular consideration of the PRL, FST, MC4R, TNNT3, MTTP and DIO3 genes), which are useful in determining the genetic background of functional traits, as well as the characteristics of the breeds for terms of genetic variation, especially conservative breeds, where it is appropriate to maintain the existing genetic diversity and intra-breed variability.
    Type of Medium: Online Resource
    ISSN: 0025-8628
    Language: Unknown
    Publisher: Medycyna Weterynaryjna - Redakcja
    Publication Date: 2017
    detail.hit.zdb_id: 2777172-6
    SSG: 22
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  • 7
    Online Resource
    Online Resource
    Medycyna Weterynaryjna - Redakcja ; 2019
    In:  Medycyna Weterynaryjna Vol. 75, No. 01 ( 2019), p. 6165-2019
    In: Medycyna Weterynaryjna, Medycyna Weterynaryjna - Redakcja, Vol. 75, No. 01 ( 2019), p. 6165-2019
    Abstract: The aim of the study was to analyze sexual activities of gilts representing various genotype groups in the RBP4 and MC4R locus. Observations of gilt behaviour during the first and the second oestrus (before initiation of the reproduction phase) were carried out in the Pulawska breed from conservation breeding. The evaluation process comprised the duration of the heat as well as the intensity of heat behaviours. Polymorphism identification in RBP4 (SNP: rs55618789) and MC4R (SNP: rs81219178) genes was performed, which resulted in the identification of three (MC4R: AA, AG and GG) and two (RBP4: CC and CT) gilt genotype groups. Significantly varying sexual activities were observed: the most intensive heat symptoms were demonstrated by CT gilts in the course of the first (P ≤ 0.05) and second (P ≤ 0.01) oestrus (locus RBP4) reaching, on average, 2.15 ± 0.13 and 2.52 ± 0.13 pt. It was further determined that the heats of CT gilts lasted longer and this difference, in the second heat, was significant (P ≤ 0.01). In the case of the MC4R locus, significant differences in gilt sexual activities were observed only in the course of the second oestrus (P ≤ 0.05). In addition, a strong association was also demonstrated of AA (locus MC4R) and CC (locus RBP4) genotypes with the thickness of the subcutaneous fat tissue (P ≤ 0.05) amounting in these gilts to 11.57 ± 0.21 mm and 11.50 ± 0.19 mm, respectively.
    Type of Medium: Online Resource
    ISSN: 0025-8628
    Language: Unknown
    Publisher: Medycyna Weterynaryjna - Redakcja
    Publication Date: 2019
    detail.hit.zdb_id: 2777172-6
    SSG: 22
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  • 8
    In: Medycyna Weterynaryjna, Medycyna Weterynaryjna - Redakcja, Vol. 78, No. 09 ( 2022), p. 6698-2022
    Abstract: The study aimed to determine the influence of NRAMP1 gene polymorphisms on the pig's productive traits. Genomic DNA was isolated from blood samples (n=50) from pigs of the Ukrainian Large White breed. Genotyping was performed at NRAMP1 loci at positions 72 and 364 (AvaII) SNP and 176 and 334 (HinfI) SNP, counting from the beginning of the amplified fragment 536 bp. Polymorphism was found at the last two these loci. For NRAMP1HinfI 334 SNP, the frequency of the allele T was 1.9 times as high as that of the allele C. The observed genotype distribution deviated significantly from the expected one for NRAMP1HinfI 334 SNP (χ2 = 10.150; p 〈 0.01). Pigs of the Ukrainian Large White breed showed a rather high NRAMP1HinfI 334 SNP polymorphism (PIC = 0.35). Relationships were found between the Exon2 of the NRAMP1 gene (HinfI 334 SNP) polymorphism and the productive traits of pigs (average daily gains, p 〈 0.05; fat thickness, p 〈 0.01). The TT genotype positively affected the growth rate of the experimental pigs from day 28 to day 120, and their average daily gains were higher by 17.7 grams or 3.2% (p 〈 0.05). At day 180, the body weight of pigs with the TT NRAMP1HinfI 334 SNP genotype was higher by 5.04 kg (p 〈 0.05). At the same time, they had thicker backfat (P 〈 0.01). 〈 /td 〉
    Type of Medium: Online Resource
    ISSN: 0025-8628
    Language: Unknown
    Publisher: Medycyna Weterynaryjna - Redakcja
    Publication Date: 2022
    detail.hit.zdb_id: 2777172-6
    SSG: 22
    Location Call Number Limitation Availability
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