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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of ornithology 137 (1996), S. 503-513 
    ISSN: 1439-0361
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Bei der Analyse der Elementrepertoires von individuell gekennzeichneten Drosselrohrsängern ergab das kumulative Auftragen neuer Elementtypen gegen die Zahl untersuchter Strophen eine deutliche Abflachung der Kurve ab der 35. Strophe. Diese Zahl muß daher als minimal notwendige Strophenzahl für die Repertoireanalyse angesehen werden. Die Repertoiregrößen von zehn Vögeln lagen zwischen 42 und 54 Elementtypen. Ein deutlicher Einfluß der Zahl der Weibchen pro Männchen auf die Repertoiregröße war nicht ersichtlich. 1993 wurden in der Teilpopulation 71 Elementtypen festgestellt, von denen 47 % so gut wie bei allen Vögeln auftraten. Die Elementsharingraten aller untersuchten Männchen-Dyaden lagen zwischen 0,69 und 0,90 und damit vergleichsweise hoch. Sie waren schwach negativ mit der Entfernung der Reviere der verglichenen Sänger korreliert. Im Laufe der Brutsaison traten bei zwei Individuen z. T. deutliche Veränderungen quantitativer Strophenparameter, des Repertoires und des Grades der Organisiertheit der Strophen auf. Ob die Vögel zu Beginn der Brutsaison eine dem Subsong ähnliche Phase durchlaufen ist unklar. Auch über zwei bzw. drei Jahre wiesen zwei Vögel z. T. erhebliche Unterschiede im dargebrachten Elementrepertoire auf. Fraglich ist, ob tatsächlich noch Änderungen des Repertoires stattfanden oder ob jeweils auf unterschiedliche Subsets des Repertoires zurückgegriffen wurde. Die Ähnlichkeit des Gesangs mit dem Müggelsee-Repertoire nahm mit dem Abstand der Herkunftsorte ab. Möglicherweise ist durch einen Umkreis von 40 km mit hoher Repertoireübereinstimmung der hauptsächliche Dismigrationsraum einer Population gekennzeichnet.
    Notes: Abstract We investigated the variability of song in the Great Reed Warbler within a colour ringed population at the Müggelsee in Berlin. The repertoires of 10 individual males contained between 42 and 54 syllable types. The males showed a relatively high rate of syllable sharing in their songs (0.69 to 0.90). These similarities show a weak negative correlation with the distance of the territories involved. Quantitative and qualitative parameters in the song of individual males changed significantly within a breeding season as well as between seasons. This indicates that the birds are either able to learn new syllables during their lives or that they use different parts of a given pool of syllables. Songs recorded within a circle of about 40 km around the study site show more similarities than between songs of birds from more distant populations. Probably, this area represents the range of regular dispersal.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2048
    Keywords: Allergenic protein ; Oryza (proteins) ; Protein (NaCl-soluble) ; Protein synthesis inhibitor ; Sodium chloride-soluble protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ten new proteins from rice (Oryza saliva L. cv. Bahia) including four protein-synthesis inhibitors and two immunoglobulin E (IgE)-binding proteins have been isolated and characterized. These proteins as well as one previously known component, α-globulin, were purified from a 0.5 M NaCl extract of rice endosperm by a new, apparently non-denaturing, isolation procedure developed for rice proteins. The method is based on extractions of this complex protein mixture with a diluted volatile salt solution and an aqueous solution of ethanol. This preliminary step results in an improvement in the separation of these proteins, thus facilitating their subsequent purification by reversed-phased high-performance liquid chromatography. These new proteins have similar relative molecular masses (Mrs) from 11000 to 17000. The purity of the proteins was analyzed by micro two-dimensional gel electrophoresis. Four of these components were found to be in-vitro protein-synthesis inhibitors in a cell-free system from rat brain. The NH2-terminal amino-acid sequences of these four inhibitors were determined from 12 to 26 cycles after direct blotting of the separated proteins from electrophoresis gels. Three of these proteins with Mrs between 16000 and 17000 showed a high degree of homology ranging from 57% to 75% but seem to be unrelated to the fourth inhibitor. In addition, the α-globulin and one of the new low-molecular-weight proteins of Mr 12500 seemed to show allergenic properties since they bound IgE antibodies from the sera of hypersensitive patients. Boths proteins have blocked NH2-terminal amino acids.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    The protein journal 11 (1992), S. 359-360 
    ISSN: 1573-4943
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-9686
    Keywords: Heart ; Left ventricle ; LV contractility ; ESPVR ; Pig ; Rat ; Magnetic resonance imaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract The end systolic pressure–volume relation (ESPVR) has been shown to be a relatively load independent measure of left ventricular (LV) contractility. Recently, several single-beat ESPVR computation methods have been developed, enabling the quantification of LV contractility without the need to alter vascular loading conditions on the heart. Using a single-beat ESPVR method, which has been validated previously in humans and assumes that normalized elastance is constant between individuals of a species, we studied the effects of myocardial infarction on LV contractility in two species, the rat and the pig. In our studies, LV pressure was acquired invasively and LV volume determined noninvasively with magnetic resonance imaging, at one week postinfarction in pigs and at 12 weeks postinfarction in rats. Normalized systolic elastance curves in both animal species were not statistically different from that of humans. Also, the slope of the ESPVR $$\left( {E_{es} } \right)$$ decreased significantly following infarction in both species, while the volume-axis intercept $$\left( {V_0 } \right)$$ was unaffected. These results indicate that a single-beat ESPVR method can be used to measure the inotropic response of the heart to myocardial infarction, and that the basis for this method (i.e., constant normalized elastance) is applicable to a variety of mammalian species. © 2000 Biomedical Engineering Society. PAC00: 8719Uv, 8761Lh, 8719Hh, 8719Rr, 8719Ff
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1439-0361
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary In 1991, a pair of Two-barred Crossbills bred successfully on a cemetery of Berlin, which is the first certain breeding record in Central Europe of this species. Two young fledged May 29. The tree stand of the cemetery consists of 80 % deciduous trees and 20 % conifers only, which means the selection of an open, mixed deciduous forest type. This event has to be seen in connection with a very intense invasion of Crossbills to Central and Western Europe. Some notes are given on plumage characters, behaviour and vocalizations.
    Notes: Zusammenfassung Der Bindenkreuzschnabel (Loxia leucoptera) brütete 1991 in einem Paar erfolgreich auf einem Berliner Friedhof und damit erstmals sicher in Mitteleuropa. Zwei Jungvögel wurden am 29. Mai flügge. Die Baumschicht des Friedhofs besteht zu 80 % aus Laubholz und nur zu 20 % aus Nadelholz, d. h. die Art wählte hier den offenen, gemischten Laubwaldtyp. Dieser Nachweis ist mit einer sehr großen Invasion von Kreuzschnäbeln nach Mittel- und Westeuropa in Verbindung zu bringen. Einige die bisherigen Erkenntnisse ergänzende Beobachtungen werden zu Gefiedermerkmalen, Verhalten und stimmlichen Äußerungen mitgeteilt.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 18 (1997), S. 723-743 
    ISSN: 0192-8651
    Keywords: Chemistry ; Theoretical, Physical and Computational Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: A Monte Carlo docking procedure that combines random displacements of the substrate and protein side chains with minimization of the enzyme - substrate complex is described and applied to finding the binding mode of the blocked tetrapeptide N-acetyl-Leu-Pro-Phe-methylamide to the FK506 binding protein (FKBP). The tetrapeptide, an analog of the preferred FKBP substrate, and the FKBP binding site are flexible during the docking procedure. The twisted-imide transition-state form of the substrate is used during docking. The enzyme charges are scaled individually to account for solvent screening of specific binding site residues during the Monte Carlo sampling. To evaluate the relative binding free energies of the resulting structures, a rapid method for calculating polar and nonpolar solvation effects is introduced. Accurate electrostatic solute - solvent energies are calculated by solving the finite-difference linearized Poisson - Boltzmann equation; nonpolar contributions to the stability of the different conformers are estimated by the free energy of cavity formation, which is obtained from the molecular surface, and the solute - solvent van der Waals energy, which is calculated with a continuum approach. In the conformation of the enzyme - substrate complex with the lowest free energy, the tetrapeptide is bound as a type VIa proline turn with solvent accessible ends to permit longer polypeptide chains to act as substrates. Except for the imide carbonyl, which is involved in polar interactions with aromatic side chains of the FKBP binding site, all of the seven potential hydrogen bond donors or acceptors of the tetrapeptide are satisfied. The FKBP binding site has a similar conformation in the substrate complex as in the FKBP-FK506 cocrystal structure, except for the predicted reorientation of the Tyr 82 hydroxyl, which plays an important role in substrate binding. The present model for the FKBP - substrate complex is in agreement with the recently determined crystal structure of a cyclic peptide - FK506 hybrid bound to FKBP and supports the structure obtained previously by iterative model building. In addition, it is consistent with the observed effects of FKBP point mutations on the enzyme activity. The approach described here should be useful, in general, for the prediction of the structure of a molecule in solution or as part of a complex. It provides for the effective sampling of conformational space and for the inclusion of solvent effects. © 1997 by John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Publication Date: 2018-05-31
    Description: Sustainability, Vol. 10, Pages 1800: Morphology Dependent Assessment of Resilience for Urban Areas Sustainability doi: 10.3390/su10061800 Authors: Kai Fischer Stefan Hiermaier Werner Riedel Ivo Häring The formation of new threats and the increasing complexity of urban built infrastructures underline the need for more robust and sustainable systems, which are able to cope with adverse events. Achieving sustainability requires the strengthening of resilience. Currently, a comprehensive approach for the quantification of resilience of urban infrastructure is missing. Within this paper, a new generalized mathematical framework is presented. A clear definition of terms and their interaction builds the basis of this resilience assessment scheme. Classical risk-based as well as additional components are aligned along the timeline before, during and after disruptive events, to quantify the susceptibility, the vulnerability and the response and recovery behavior of complex systems for multiple threat scenarios. The approach allows the evaluation of complete urban surroundings and enables a quantitative comparison with other development plans or cities. A comprehensive resilience framework should cover at least preparation, prevention, protection, response and recovery. The presented approach determines respective indicators and provides decision support, which enhancement measures are more effective. Hence, the framework quantifies for instance, if it is better to avoid a hazardous event or to tolerate an event with an increased robustness. An application example is given to assess different urban forms, i.e., morphologies, with consideration of multiple adverse events, like terrorist attacks or earthquakes, and multiple buildings. Each urban object includes a certain number of attributes, like the object use, the construction type, the time-dependent number of persons and the value, to derive different performance targets. The assessment results in the identification of weak spots with respect to single resilience indicators. Based on the generalized mathematical formulation and suitable combination of indicators, this approach can quantify the resilience of urban morphologies, independent of possible single threat types and threat locations.
    Electronic ISSN: 2071-1050
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by MDPI Publishing
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  • 8
    Publication Date: 2013-08-07
    Description: Plasmacytoid dendritic cells (pDCs) play an important role in innate and adaptive immunity and were shown to be identical to previously described natural IFN-α-producing (NIP) cells. Here, we describe two functionally distinct pDC subpopulations that are characterized by the differential expression of stem cell antigen-1 (Sca-1; Ly-6A/E). Sca-1 − pDCs are mainly found in the bone marrow, appear first during development, show a higher proliferative activity and represent the more precursor phenotype. Sca-1 + pDCs are mostly located in secondary lymphoid organs and represent a later developmental stage. Sca-1 − pDCs give rise to a Sca-1 + subset upon activation or in response to endogenous type I IFN. Interestingly, in contrast to Sca-1 − pDCs, Sca-1 + pDCs are defective in IFN-α production upon endosomal TLR9 stimulation, whereas lysosomal signaling via TLR9 is functional in both subsets. Gene expression analysis revealed that osteopontin (Opn) is strongly upregulated in Sca-1 − pDCs. These data provide evidence for the molecular basis of the observed functional heterogeneity, as the intracellular isoform of Opn couples TLR9 signaling to IFN-α expression. Taken together, our results indicate that Sca-1 − pDCs are an early developmental stage of pDCs with distinct innate functions representing the true murine NIP cell.
    Print ISSN: 0014-2980
    Electronic ISSN: 1521-4141
    Topics: Medicine
    Published by Wiley-Blackwell
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  • 9
    Publication Date: 2018-01-25
    Description: In order to identify anaplastic lymphoma kinase-driven non-small cell lung cancer (ALK + NSCLC) patients with a worse outcome, who might require novel therapeutic approaches, we retrospectively analyzed all stage IV cases treated at our institutions with one of the main echinoderm microtubule-associated protein-like 4 (EML4)-ALK fusion variants V1, V2, V3 as detected by next-generation sequencing (NGS) or RT-PCR (n=67). Progression under tyrosine kinase inhibitor (TKI) treatment was evaluated both according to Response Evaluation Criteria in Solid Tumors (RECIST) and by the need to change systemic therapy. EML4-ALK fusion variants V1, V2 and V3 were found in 39%, 10% and 51% of cases respectively. Patients with V3-driven tumors had more metastatic sites at diagnosis than cases with the V1 and V2 variants (mean 3.3 vs. 1.9 and 1.6, p=0.005), which suggests increased disease aggressiveness. Furthermore, V3-positive status was associated with earlier failure after treatment with first and second generation ALK TKI (median progression-free survival [PFS] by RECIST in the first line 7.3 vs. 39.3 months, p=0.01), platinum-based combination chemotherapy (median PFS 5.4 vs. 15.2 months for the first line, p=0.008) and cerebral radiotherapy (median brain PFS 6.1 months vs. not reached for cerebral radiotherapy during first-line treatment, p=0.028), and with inferior overall survival (39.8 vs. 59.6 months in median, p=0.017). Thus, EML4-ALK fusion variant V3 is a high-risk feature for ALK + NSCLC. Determination of V3 status should be considered as part of the initial workup for this entity in order to select patients for more aggressive surveillance and treatment strategies. This article is protected by copyright. All rights reserved.
    Print ISSN: 0020-7136
    Electronic ISSN: 1097-0215
    Topics: Biology , Medicine
    Published by Wiley-Blackwell
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  • 10
    Publication Date: 2014-05-14
    Description: Obtaining functional information on the human lung is of tremendous interest in the characterization of lung defects and pathologies. However, pulmonary ventilation and perfusion maps usually require contrast agents and the application of electrocardiogram (ECG) triggering and breath holds to generate datasets free of motion artifacts. This work demonstrates the possibility of obtaining highly resolved perfusion-weighted and ventilation-weighted images of the human lung using proton MRI and the SElf-gated Non-Contrast-Enhanced FUnctional Lung imaging (SENCEFUL) technique. The SENCEFUL technique utilizes a two-dimensional fast low-angle shot (FLASH) sequence with quasi-random sampling of phase-encoding (PE) steps for data acquisition. After every readout, a short additional acquisition of the non-phase-encoded direct current (DC) signal necessary for self-gating was added. By sorting the quasi-randomly acquired data according to respiratory and cardiac phase derived from the DC signal, datasets of representative respiratory and cardiac cycles could be accurately reconstructed. By application of the Fourier transform along the temporal dimension, functional maps (perfusion and ventilation) were obtained. These maps were compared with dynamic contrast-enhanced (DCE, perfusion) as well as standard Fourier decomposition (FD, ventilation) reference datasets. All datasets were additionally scored by two experienced radiologists to quantify image quality. In addition, one initial patient examination using SENCEFUL was performed. Functional images of healthy volunteers and a patient diagnosed with hypoplasia of the left pulmonary artery and left-sided pulmonary fibrosis were successfully obtained. Perfusion-weighted images corresponded well to DCE-MRI data; ventilation-weighted images offered a significantly better depiction of the lung periphery compared with standard FD. Furthermore, the SENCEFUL technique hints at a potential clinical relevance by successfully detecting a perfusion defect in the patient scan. It can be concluded that SENCEFUL enables highly resolved ventilation- and perfusion-weighted maps of the human lung to be obtained using proton MRI, and might be interesting for further clinical evaluation. Copyright © 2014 John Wiley & Sons, Ltd. SElf-gated Non-Contrast-Enhanced FUnctional Lung imaging (SENCEFUL) is a non-contrast-enhanced technique to obtain highly resolved images of lung morphology, ventilation and perfusion. SENCFUL involves the acquisition of the direct current (DC) self-gating signal which was additionally recorded during imaging in free breathing without electrocardiogram (ECG) triggering. The data are retrospectively sorted according to the DC signal, thereby obtaining time-resolved datasets of cardiac and respiratory motion, which allow the calculation of qualitative functional lung maps by applying the Fourier transform, similar to Fourier decomposition.
    Print ISSN: 0952-3480
    Electronic ISSN: 1099-1492
    Topics: Medicine
    Published by Wiley-Blackwell
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