Description: Draft genome sequence of the Tibetan antelope Nature Communications 4, 1858 (2013). doi:10.1038/ncomms2860 Authors: Ri-Li Ge, Qingle Cai, Yong-Yi Shen, A San, Lan Ma, Yong Zhang, Xin Yi, Yan Chen, Lingfeng Yang, Ying Huang, Rongjun He, Yuanyuan Hui, Meirong Hao, Yue Li, Bo Wang, Xiaohua Ou, Jiaohui Xu, Yongfen Zhang, Kui Wu, Chunyu Geng, Weiping Zhou, Taicheng Zhou, David M. Irwin, Yingzhong Yang, Liu Ying, Haihua Bao, Jaebum Kim, Denis M. Larkin, Jian Ma, Harris A. Lewin, Jinchuan Xing, Roy N. Platt, David A. Ray, Loretta Auvil, Boris Capitanu, Xiufeng Zhang, Guojie Zhang, Robert W. Murphy, Jun Wang, Ya-Ping Zhang & Jian Wang

Description: Nature Protocols 7, 2080 (2012). doi:10.1038/nprot.2012.115 Authors: Ting Zhou, Christina Benda, Sarah Dunzinger, Yinghua Huang, Jenny Cy Ho, Jiayin Yang, Yu Wang, Ya Zhang, Qiang Zhuang, Yanhua Li, Xichen Bao, Hung-Fat Tse, Johannes Grillari, Regina Grillari-Voglauer, Duanqing Pei & Miguel A Esteban Human induced pluripotent stem cells (iPSCs) have been generated with varied efficiencies from multiple tissues. Yet, acquiring donor cells is, in most instances, an invasive procedure that requires laborious isolation. Here we present a detailed protocol for generating human iPSCs from exfoliated renal epithelial cells

Description: Medical staff in the department of obstetrics and gynecology are a group of professionals reportedly at high risk of work-related musculoskeletal disorders (WMSD), however, little is known about the current status of this problem in China. The aim of this study was to investigate prevalence and risk factors of work-related musculoskeletal disorders among this population in China. A self-developed questionnaire was distributed to 1017 obstetrics and gynecology practitioners to collect information on musculoskeletal symptoms and relevant factors. Prevalence and severity of work-related musculoskeletal disorders in different parts of the body were calculated and the relationship between personal and ergonomic factors and work-related musculoskeletal disorders was analyzed using Chi-square test and unconditional logistic regression models. The results indicated a high prevalence of 85.5% among the subjects, with the shoulder (n = 575, 62.0%), neck (n = 560, 60.3%) and lower back (n = 504, 54.3%) being the three most affected regions. Individual, postural, work-environmental as well as psychosocial factors were recognized to be associated with WMSDs in different body parts. Therefore, attention must be given to the problem of musculoskeletal disorders among Chinese obstetrics and gynecology staff. It is recommended to develop good life habits, improve work environment, adjust work organization as well as train on proper postures in their daily operation.

Description: The estimation of soil organic carbon (SOC) stock in terrestrial ecosystems of China is of particular importance because it exerts a major influence on worldwide terrestrial carbon (C) storage and global climate change. Map-based estimates of SOC stocks conducted in previous studies have typically been applied on planimetric areas, which led to the underestimation of SOC stock. In the present study, SOC stock in China was estimated using a revised method on three-dimensional (3-D) surfaces, which considered the undulation of the landforms. Data were collected from the 1:4 M China Soil Map and a search work from the Second Soil Survey in China. Results indicated that the SOC stocks were 28.8 Pg C and 88.5 Pg C in soils at depths of 0–20 cm and 0–100 cm, corresponding to significant increases of 5.66% and 5.44%, respectively. Regression analysis revealed that the SOC stock accumulated with the increase of areas on 3-D surfaces. These results provide more reasonable estimates and new references about SOC stocks in terrestrial ecosystems of China. The method of estimation on 3-D surfaces has scientific meaning to promote the development of new approaches to estimate accurate SOC stocks.

Description: Water, Vol. 9, Pages 966: Climate Change Impacts on Flow and Suspended Sediment Yield in Headwaters of High-Latitude Regions—A Case Study in China’s Far Northeast Water doi: 10.3390/w9120966 Authors: Yuyan Zhou Y. Xu Weihua Xiao Jianhua Wang Ya Huang Heng Yang Climate change is expected to have stronger effects on water resources in higher latitude regions. Despite intensive research on possible hydrological responses in those regions to a warmer environment, our knowledge on erosion and sediment yield induced by the climate change in high-latitude headwaters is still limited. In this study, we estimated suspended sediment yields from 2021 to 2050 in a typical headwater area of far Northeast China to elucidate potential impacts of future climate change on surface runoff and erosion in higher latitude regions. We first parameterized the Soil and Water Assessment Tool (SWAT) using historical measurements to estimate runoff from the river basin. The model performed well in both the calibration (2006–2011) and the validation (2012–2014) periods, with an R2 of 0.85 and 0.88 and a Nash-Sutcliffe Efficiency (NSE) of 0.7 and 0.73, respectively. We also utilized historical measurements on sediment yields from the period 2006–2014 to develop a runoff-sediment yield rating curve, and the rating curve obtained an excellent goodness of fit (R2 = 0.91, p < 0.001). We then applied the calibrated SWAT model to two climate change projections, also known as Representative Concentration Pathways (RCP4.5 and RCP8.5), for the period from 2021 to 2050 to obtain future runoff estimates. These runoff estimates were then used to predict future sediment yield by using the developed runoff-sediment yield rating curve. Our study found a significant increase of annual sediment yield (p < 0.05) for both climate change projections (RCP4.5 = 237%; RCP8.5 = 133%) in this, China’s high-latitude region. The increases of sediment yield were prevalent in summer and autumn, varying from 102–299% between the two RCPs scenarios. Precipitation was the dominated factor that determined the variation of runoff and sediment yield. A warming climate could bring more snowmelt-induced spring runoff and longer rainy days in autumn, hence leading to higher erosion. These findings demonstrate that under the changing climate, soils in this high-latitude headwater area would be eroded twice to three times that of the baseline period (1981–2010), indicating a potential risk to the downstream water quality and reservoir management.

Description: Sustainability, Vol. 10, Pages 2701: Factors Causing Farmland Price-Value Distortion and Their Implications for Peri-Urban Growth Management Sustainability doi: 10.3390/su10082701 Authors: Yu-Hui Chen Chun-Lin Lee Guan-Rui Chen Chiung-Hsin Wang Ya-Hui Chen Taiwan’s Agricultural Development Act (ADA) of 2000 relaxed farmland ownership criteria and allowed non-farmers to own farms. Although this opened up the market and induced a growth in farmland trading, relaxing these criteria without proper monitoring resulted in rapid development of farmhouses that fragmented farmlands, adversely affecting agricultural production and the quality of peri-urban environments, and increased management difficulties. Relaxing farmland ownership criteria also provided opportunities for speculation, which pushed up farmland prices, causing farmland price to deviate from its production value. We used a price:value ratio as an index of price-value distortion to explore farmland price-value distortion spatially using a geographical information system (GIS). Yilan County was used as a case study since its agricultural lands suffer high development pressure due to ready accessibility from the Taipei metropolitan area. Ordinary least square and quantile regression were used to identify factors driving distortion in Yilan County. Finally, we discuss the distortion and key factors for specific sites in Yilan to assess the urban sprawl and propose a preliminary course of action for peri-urban growth management. Our findings suggest that residential activities stimulate farmland price-value distortion but do not enhance farmland value. Designation of a land parcel as agricultural within an urban area allows for speculation and increases distortion. The land parcel’s association with infrastructure such as road and irrigation systems, and the price of agricultural products, are significantly correlated with distortion. Most of these identified factors increased farmland price because of the potential for non-agricultural land-use. We propose that to resolve farmland price-value distortion in Yilan, multi-functional values, in addition to agriculture, must be envisioned.

Description: Visually impaired people have difficulty accessing information about public transportation systems. Several systems have been developed for assisting visually impaired and blind people to use the city bus. Most systems provide only one-way communication and require high-cost and complex equipment. The purpose of this study is to reduce the difficulties faced by visually impaired people when taking city buses, using an interactive wireless communication system. The system comprised a user module and a bus module to establish a direct one-to-one connection. When the user inputs 4-digit numbers, the user module immediately sends out the information. If the bus module receives the matched bus number, it buzzes and the warning LED flashes to notify the bus driver that someone is waiting to board on the bus. User tests were conducted by two visually impaired people in a simulated vehicle and a city bus. The success rate of interactive wireless communication, recognizing the arrival of the bus and boarding the correct bus reached 100% in all of the tests. The interactive wireless communication aid system is a valid and low-cost device for assisting visually impaired people to use city buses.

Description: ERCC2 is indispensable for nucleotide excision repair pathway, and its functional polymorphisms may be associated with cancer risk. In a large case-control study of 1126 esophageal squamous cell carcinomas (ESCC) patients and 1131 controls, we genotyped two SNPs in ERCC2 (rs238406 G 〉 T and rs13181 T 〉 G) and assessed their associations with ESCC risk. We found a significantly elevated ESCC risk associated with the rs238406 T variant genotypes (adjusted OR = 1.30 and 1.24, 95% CI = 1.02–1.66 and 1.03–1.49 for TG and TG/TT, respectively, compared with GG), particularly in the subgroup of those smoked more than 16 pack-years. Multivariate logistic regression analysis suggested a possible multiplicative gene-environment interaction between rs238406 genotypes and smoking (Pinteraction = 0.026) on ESCC risk. Although no significant risk associations were observed for rs13181, further mini meta-analysis with our and 18 other published studies of 5,012 cases and 8,238 controls found evidence of an association between the rs13181 variant G allele and esophageal cancer risk (TG/GG vs. TT, OR = 1.17; 95% CI = 1.02–1.33). Interestingly, we consistently found a significant correlation between variant genotypes of these two SNPs and ERCC2 mRNA expression. These findings suggest that potentially functional SNPs in ERCC2 may contribute to ESCC risk. Scientific Reports 4 doi: 10.1038/srep06281

Description: This study analyzes the duration and division of the flood season in the Fenhe River Basin over the period of 1957–2014 based on daily precipitation data collected from 14 meteorological stations. The Mann–Kendall detection, the multiscale moving t-test, and the Fisher optimal partition methods are used to evaluate the impact of climate change on flood season duration and division. The results show that the duration of the flood season has extended in 1975–2014 compared to that in 1957–1974. Specifically, the onset date of the flood season has advanced 15 days, whereas the retreat date of the flood season remains almost the same. The flood season of the Fenhe River Basin can be divided into three stages, and the variations in the onset and retreat dates of each stage are also examined. Corresponding measures are also proposed to better utilize the flood resources to adapt to the flood season variations.

Description: Epidemiologic characteristics, knowledge and risk factors of unintentional burns in rural children in Zunyi, Southwest China Scientific Reports, Published online: 17 October 2016; doi:10.1038/srep35445