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  • 1
    In: Agronomy, MDPI AG, Vol. 10, No. 2 ( 2020-02-01), p. 195-
    Abstract: In the field of pesticide spraying, droplet size is one of the most important factors affecting droplet deposition and drift. In order to study the effect of different droplet size parameters on droplet deposition distribution and drift of aerial spraying by using plant protection UAV, an aerial spraying test with the same spraying rate and different size droplets in rice canopy was carried out by using multi-rotor unmanned aerial vehicles (UAV) and four TEEJET nozzles with different orifice sizes (these droplets with a volume median diameter (VMD) of 95.21, 121.43, 147.28, and 185.09 μm, respectively), and the deposition distribution and penetration of droplets in the target area and the drift distribution of droplets in the non-target area were compared and analyzed. The results showed that the deposition distribution and penetration of droplets in the target area and the drift distribution of droplets in the non-target area were influenced by the droplet size. The droplet deposition rate in the upper and lower rice canopies were increased in the target area with the increase of droplet size. The penetration results of droplets also increased with the increase of droplet size, and that of droplets with a VMD of 185.09 μm was the best, reaching 38.13%. The average values of the cumulative drift rate of droplets in the rice canopy in the four tests were 73.87%, 50.26%, 35.91%, and 23.06%, respectively, and the cumulative drift rate and the drift distance of droplets decreased with the increase of droplet size, which indicated that the increase of droplet size can effectively reduce droplet drift. It demonstrated that the droplet size is one of the most important factors affecting droplet deposition and drift for pesticide spraying by plant protection UAV, and for the application of plant protection UAV with extra-low volume spraying, the use of droplets with VMD less than 160 μm should be avoided and a more than 10 m buffer zone should be considered downwind of the spraying field to avoid drug damage caused by pesticide drift. The results have fully revealed the effect of droplet size parameters on droplet deposition and drift of aerial spraying. Moreover, the influence of the wind field below the rotors on the distribution of droplet deposition was surmised and analyzed from the perspective of plant protection UAV. It is important for optimizing the droplet parameters of aerial spraying, increasing the spraying efficiency, and realizing precision agricultural aviation spray.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2607043-1
    SSG: 23
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  • 2
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 24, No. 13 ( 2023-07-06), p. 11160-
    Abstract: Most children with a neurogenic bladder (NB) have bladder fibrosis, which causes irreversible bladder dysfunction and damage to the upper urinary tract. However, the mechanism of bladder fibrosis remains unclear. This study aimed to investigate the underlying causes of bladder fibrosis. Here, the lumbar 6 (L6) and sacral 1 (S1) spinal nerves of Sprague Dawley rats were severed bilaterally to establish NB models. Using RNA-seq, we discovered that the NF-κB signaling pathway and inflammation were upregulated in spinal cord injury (SCI)-induced bladder fibrosis. Subsequent Western blotting, enzyme-linked immunosorbent assays, immunohistochemical staining, and immunofluorescence staining verified the RNA-seq findings. To further clarify whether the NF-κB signaling pathway and pyroptosis were involved in bladder fibrosis, a TGF-β1-treated urinary epithelial cell line (SV-HUC-1 cells) was used as an in vitro model. Based on the results of RNA-seq, we consistently found that the NF-κB signaling pathway and pyroptosis might play important roles in TGF-β1-treated cells. Further experiments also confirmed the RNA-seq findings in vitro. Moreover, using the NLRP3 inhibitor MCC950 rescued TGF-β1-induced fibrosis, and the NF-κB signaling pathway inhibitor BAY 11-7082 effectively rescued TGF-β1-induced pyroptosis and the deposition of extracellular matrix by SV-HUC-1 cells. In summary, our research demonstrated for the first time that the NF-κB signaling pathway inhibition rescued bladder epithelial cells pyroptosis and fibrosis in neurogenic bladders.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 3
    In: Cells, MDPI AG, Vol. 11, No. 23 ( 2022-11-29), p. 3836-
    Abstract: Background: Neurogenic bladder (NB) patients exhibit varying degrees of bladder fibrosis, and the thickening and hardening of the bladder wall induced by fibrosis will further affect bladder function and cause renal failure. Our study aimed to investigate the mechanism of bladder fibrosis caused by a spinal cord injury (SCI). Methods: NB rat models were created by cutting the bilateral lumbar 6 (L6) and sacral 1 (S1) spinal nerves. RNA-seq, Western blotting, immunofluorescence, cell viability and ELISA were performed to assess the inflammation and fibrosis levels. Results: The rats showed bladder dysfunction, upper urinary tract damage and bladder fibrosis after SCI. RNA-seq results indicated that hypoxia, EMT and pyroptosis might be involved in bladder fibrosis induced by SCI. Subsequent Western blot, ELISA and cell viability assays and immunofluorescence of bladder tissue confirmed the RNA-seq findings. Hypoxic exposure increased the expression of HIF-1α and induced EMT and pyroptosis in bladder epithelial cells. Furthermore, HIF-1α knockdown rescued hypoxia-induced pyroptosis, EMT and fibrosis. Conclusion: EMT and pyroptosis were involved in the development of SCI-induced bladder fibrosis via the HIF-1α pathway. Inhibition of the HIF-1α pathway may serve as a potential target to alleviate bladder fibrosis caused by SCI.
    Type of Medium: Online Resource
    ISSN: 2073-4409
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2661518-6
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  • 4
    In: Viruses, MDPI AG, Vol. 15, No. 8 ( 2023-08-18), p. 1761-
    Abstract: OTUD6A is a deubiquitinase that plays crucial roles in various human diseases. However, the precise regulatory mechanism of OTUD6A remains unclear. In this study, we found that OTUD6A significantly inhibited the production of type I interferon. Consistently, peritoneal macrophages and bone marrow-derived macrophages from Otud6a−/− mice produced more type I interferon after virus infection compared to cells from WT mice. Otud6a−/−− mice also exhibited increased resistance to lethal HSV-1 and VSV infections, as well as LPS attacks due to decreased inflammatory responses. Mechanistically, mass spectrometry results revealed that UBC13 was an OTUD6A-interacting protein, and the interaction was significantly enhanced after HSV-1 stimulation. Taken together, our findings suggest that OTUD6A plays a crucial role in the innate immune response and may serve as a potential therapeutic target for infectious disease.
    Type of Medium: Online Resource
    ISSN: 1999-4915
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2516098-9
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