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  • 1
    In: Nutrients, MDPI AG, Vol. 15, No. 2 ( 2023-01-16), p. 468-
    Abstract: We studied the activities of Siraitia grosvenorii extracts (SGE) on airway inflammation in a mouse model of chronic obstructive pulmonary disease (COPD) stimulated by cigarette smoke extract (CSE) and lipopolysaccharide (LPS), as well as in LPS-treated human bronchial epithelial cell line (BEAS-2B). SGE improved the viability of LPS-incubated BEAS-2B cells and inhibited the expression and production of inflammatory cytokines. SGE also attenuated the mitogen-activated protein kinase (MAPK)-nuclear factor-kappa B (NF-κB) signaling activated by LPS stimulation in BEAS-2B cells. In mice stimulated by CSE and LPS, we observed the infiltration of immune cells into the airway after COPD induction. SGE reduced the number of activated T cells, B cells, and neutrophils in bronchoalveolar fluid (BALF), lung tissue, mesenteric lymph node, and peripheral blood mononuclear cells, as well as inhibited infiltration into organs and mucus production. The secretion of cytokines in BALF and the expression level of pro-inflammatory cytokines, mucin 5AC, Transient receptor potential vanilloid 1, and Transient receptor potential ankyrin 1 in lung tissue were alleviated by SGE. In addition, to investigate the activity of SGE on expectoration, we evaluated phenol red secretions in the trachea of mice. SGE administration showed the effect of improving expectoration through an increase in phenol red secretion. Consequently, SGE attenuates the airway inflammatory response in CSE/LPS-stimulated COPD. These findings indicate that SGE may be a potential herbal candidate for the therapy of COPD.
    Type of Medium: Online Resource
    ISSN: 2072-6643
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2518386-2
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  • 2
    In: Agronomy, MDPI AG, Vol. 12, No. 9 ( 2022-08-29), p. 2056-
    Abstract: Saponarin content in barley sprouts may vary greatly with environmental conditions, such as climate, leading to difficulty in uniformly producing saponarin-rich barley sprouts in situ farmlands throughout the year. This research was an early attempt to identify the optimal conditions of various climatic factors, such as temperature, light, and humidity according to seasonal change, for maximizing the saponarin content of sprouted barley through the two-year field experiment. As a result, the growth index, as leaf length relative to growth period, of barley sprouts varied greatly with sowing time, and they tended to decrease with an increase in the ambient temperature, such as average daily temperature. In contrast, higher saponarin contents were observed in the sprouts collected in March, April, September, and October than those collected from May to August. We also found significantly positive correlations of saponarin content with daily temperature range and average light period, indicating that they could be decisive climatic factors for the production of barley sprouts with a higher saponarin content. Interestingly, the polynomial relationship between saponarin yield and leaf length showed the highest yield with 2.18 mg plant−1 at 15.9 cm in length, suggesting a best cutting time for the production of saponarin-rich barely sprouts based on the leaf length. Overall, the decisive climatic factors according to seasonal change for saponarin biosynthesis may be considered to be daily temperature differences and light hours.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2607043-1
    SSG: 23
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