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  • MDPI AG  (49)
  • 1
    In: Agronomy, MDPI AG, Vol. 10, No. 4 ( 2020-03-27), p. 465-
    Abstract: In the southern Huang-Huai-Hai (HHH) region, China, maize production is frequently threatened by waterlogging at the seedling stage and by drought at the big flare stage. A two-year field experiment was performed to explore whether subsoiling (SS) in the winter wheat season could improve the photosynthetic capacity and increase the water use efficiency (WUE) of summer maize using the variety, Luyu9105. A split design was adopted in the experiment. The main plots used tillage practices, including SS and rotary tillage (RT). The subplots consisted of two irrigation methods, i.e., applied supplemental irrigation at the big flare stage (I) and no irrigation at the big flare stage (NI). The results showed that the SS treatment significantly increased soil water content (SWC) in the 40–60 cm soil layer. The SS treatment improved green leaf area index (gLAI) by 15.1%–30.2%, and enhanced the ear-leaf net photosynthetic rate (Pn), stomatal conductance (gs), intercellular CO2 concentration (ci) and transpiration rate (Tr), and was accompanied by an increase in the grain-filling duration (T) by 5 days and the mean grain-filling rate (Va). The SS treatment decreased the stomatal limitation (Ls), indicating that RT treatment, which was under lower SWC, led to a decrease in Pn. Applied supplemental irrigation under RT treatment was able to compensate for the growth of leaves, but could not reverse the decreasing trend in the gLAI. Ultimately, the SS treatment improved WUE by 9.1%–9.9%, and increased grain yields by 10.0%–29.3%. Therefore, this study showed that in the southern Huang-Huai-Hai Plain, which has a yellow cinnamon soil type, the practice of SS can improve the photosynthetic characteristics of leaves and WUE of rainfed summer maize.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2607043-1
    SSG: 23
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  • 2
    In: Cells, MDPI AG, Vol. 11, No. 24 ( 2022-12-12), p. 4013-
    Abstract: Dynein axonemal heavy chain 5 (DNAH5) is the most mutated gene in primary ciliary dyskinesia (PCD), leading to abnormal cilia ultrastructure and function. Few studies have revealed the genetic characteristics and pathogenetic mechanisms of PCD caused by DNAH5 mutation. Here, we established a child PCD airway organoid directly from the bronchoscopic biopsy of a patient with the DNAH5 mutation. The motile cilia in the organoid were observed and could be stably maintained for an extended time. We further found abnormal ciliary function and a decreased immune response caused by the DNAH5 mutation through single-cell RNA sequencing (scRNA-Seq) and proteomic analyses. Additionally, the directed induction of the ciliated cells, regulated by TGF-β/BMP and the Notch pathway, also increased the expression of inflammatory cytokines. Taken together, these results demonstrated that the combination of multiomics analysis and organoid modelling could reveal the close connection between the immune response and the DNAH5 gene.
    Type of Medium: Online Resource
    ISSN: 2073-4409
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2661518-6
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  • 3
    In: Agronomy, MDPI AG, Vol. 13, No. 3 ( 2023-03-14), p. 846-
    Abstract: The sustainable high yield of crops is critically important under the current situation of global climate warming. In order to improve regional yield, it is urgent to clarify the limiting factors of local grain yield and change the traditional planting measurements to adapt to the warming climate and make full use of climate resources. Long-term field experiments over seven years from 2014 to 2021 were conducted with the same maize cultivar (i.e., Luyu9105) with seven planting density treatments: 3.0 × 104 (D1), 4.5 × 104 (D2), 6.0 × 104 (D3), 7.5 × 104 (D4), 9.0 × 104 (D5), 10.5 × 104 (D6), and 12.0 × 104 (D7) plants per hectare in Taihe and Hefei, which belong to the southern Huang-Huai-Hai (SHHH) and southeast (SE) maize-producing areas in China. According to the field experiment data, differences in grain yield, ear number, kernel number per spike, and 1000-kernel weight of different treatments were analyzed. The utilization efficiency of climate resources in Taihe and Hefei was calculated using daily solar radiation, mean temperature, and precipitation data. The results showed that Taihe had 7.8% higher solar radiation during the growing season of maize than Hefei, while accumulated temperature ≥10 °C (AT10) was 3.9% lower than Hefei. The grain yields of different planting densities in Taihe were 9.7~23.6% higher than in Hefei. The agronomic optimal planting density (AOPD) was 8.6 × 104 plants ha−1 in Taihe and 8.0 × 104 plants ha−1 in Hefei. Compared to the actual grain yields, when the agronomic optimal planting densities were adopted, the simulated yield increased by 51.3% and 59.6%, respectively. The radiation utilization efficiency, temperature utilization efficiency, and precipitation utilization efficiency in Taihe were 12.9%, 24.6%, and 26.7% higher than the values of Hefei, respectively, and D4 and D5 treatments had significantly higher climatic resource utilization efficiency than D1 and D2 treatment. The grain yield was negatively correlated with accumulated temperature ≥10 °C and positively correlated with solar radiation. The multiple linear regression model among solar radiation, accumulated temperature was ≥10 °C, and grain yield was y = 0.550R−0.562AT10 + 14,593.6 (R = 0.379). Accumulated temperature ≥10 °C was the main climatic factor affecting the grain yield due to the higher occurrence probability of a maximum temperature ≥35 °C. Overall, in the future, increasing planting density and alleviating heat stress may enhance grain yield. These results could provide cultivation measurements with regional characteristics to adapt to the local climate and maximize the utilization efficiency of climatic resources.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2607043-1
    SSG: 23
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  • 4
    Online Resource
    Online Resource
    MDPI AG ; 2022
    In:  International Journal of Environmental Research and Public Health Vol. 19, No. 23 ( 2022-11-25), p. 15703-
    In: International Journal of Environmental Research and Public Health, MDPI AG, Vol. 19, No. 23 ( 2022-11-25), p. 15703-
    Abstract: Soil erodibility research is of theoretical and practical significance to the prediction and prevention of regional soil erosion. At present, the study on soil erodibility in the lateritic red soil area of eastern Guangdong province is relatively lacking. Taking the forest land soil of the Yinna mountainous area as the research object, the physical and chemical properties (organic matter mass fraction, texture, moisture, bulk density, pH, aggregate content) of soil samples at different altitudes were measured with field survey sampling and indoor analysis. Soil erodibility K values were simulated with different models (the EPIC model, the Torri model, and the Shirazi model) and the regional applicability of the K simulation models was discussed. The influence of soil properties on soil erodibility was analyzed. The results showed that: (1) K values in the Yinna mountainous area are between 0.0250 and 0.0331 t·hm2·h/MJ·mm·hm2, and the K value in the subsoil layer (20–40 cm) is higher than that of the topsoil layer (0–20 cm). These values decreased significantly with the increase of altitude. The soil in the study area belongs to low–medium to medium erodible soil types. (2) The three models have certain applicability in the Yinna mountainous area, but the simulation results still lack validation. (3) Soil particle size composition is the most important factor affecting the K value in the study area. As far as the topsoil is concerned, K values increase with the increase of clay and silt content and decrease with the increase of sand content and aggregate stability. Soil erodibility has no significant correlation with pH and bulk density and has no clear relationship with the content of soil organic carbon and soil moisture. The research results can provide basic data for regional soil and water conservation and the construction of K value databases of different soil types in China.
    Type of Medium: Online Resource
    ISSN: 1660-4601
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2175195-X
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  • 5
    In: Journal of Clinical Medicine, MDPI AG, Vol. 12, No. 8 ( 2023-04-14), p. 2863-
    Abstract: Periprosthetic joint infection (PJI) is a devastating complication. This study aimed to unravel the veil of the N6-methyladenine (m6A) modification in PJI. Synovium, synovial fluid, sonication fluid and bone samples were collected intraoperatively from Staphylococcus aureus PJI and aseptic failure (AF) patients. The overall m6A level was detected by the m6A RNA methylation quantification kit, and the expression of m6A-related genes was quantified by real-time PCR and Western blot. Finally, an epitranscriptomic microarray and bioinformatics analysis were performed. We showed that there was a significant difference in overall m6A level between the PJI group and the AF group (PJI group had a higher overall m6A level). The expression level of METTL3 was higher in the PJI group than that in the AF group. There were 2802 differential m6A-modified mRNAs. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that differential m6A-modified mRNAs were significantly enriched in the NOD-like receptor signaling pathway, Th17 cell differentiation and the IL-17 signaling pathway, which indicates that the m6A modification might be involved in the processes of infection and immune response, bone metabolism and programmed cell death in PJI. In summary, the present work demonstrated that m6A modification plays a role in PJI and might be a therapeutic target for developing effective treatment strategies.
    Type of Medium: Online Resource
    ISSN: 2077-0383
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2662592-1
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  • 6
    In: Molecules, MDPI AG, Vol. 23, No. 5 ( 2018-04-24), p. 989-
    Type of Medium: Online Resource
    ISSN: 1420-3049
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2008644-1
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  • 7
    Online Resource
    Online Resource
    MDPI AG ; 2013
    In:  International Journal of Molecular Sciences Vol. 14, No. 9 ( 2013-09-10), p. 18615-18628
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 14, No. 9 ( 2013-09-10), p. 18615-18628
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2013
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 8
    In: Micromachines, MDPI AG, Vol. 14, No. 3 ( 2023-02-21), p. 505-
    Abstract: In conventional parameters design, the driving circuit is usually simplified as an RLC second-order circuit, and the switching characteristics are optimized by selecting parameters, but the influence of switching characteristics on the driving circuit is not considered. In this paper, the insight mechanism for the gate-source voltage changed by overshoot and ringing caused by the high switching speed of SiC MOSFET is highlighted, and we propose an optimized design method to obtain optimal parameters of the SiC MOSFET driving circuit with consideration of parasitic parameters. Based on the double-pulse circuit, we evaluated the influence of main parameters on the gate-source voltage, including driving voltage, driving resistance, gate parasitic inductance, and stray inductance of the power circuit. A SiC-based boost PFC is constructed and tested. The test results show that the switching loss can be reduced by 7.282 W by using the proposed parameter optimization method, and the over-voltage stress of SiC MOSFET is avoided.
    Type of Medium: Online Resource
    ISSN: 2072-666X
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2620864-7
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  • 9
    In: Biosensors, MDPI AG, Vol. 12, No. 9 ( 2022-09-01), p. 706-
    Abstract: The COVID-19 pandemic poses a threat to global health. Due to its high sensitivity, specificity, and stability, real-time fluorescence quantitative (real-time PCR) detection has become the most extensively used approach for diagnosing SARS-CoV-2 pneumonia. According to a report from the World Health Organization, emerging and underdeveloped nations lack nucleic acid detection kits and polymerase chain reaction (PCR) instruments for molecular biological detection. In addition, sending samples to a laboratory for testing may result in considerable delays between sampling and diagnosis, which is not favorable to the timely prevention and control of new crown outbreaks. Concurrently, there is an urgent demand for accurate PCR devices that do not require a laboratory setting, are more portable, and are capable of completing testing on-site. Hence, we report on HDLRT-qPCR, a new, low-cost, multiplexed real-time fluorescence detection apparatus that we have developed for on-site testing investigations of diverse diseases in developing nations. This apparatus can complete on-site testing rapidly and sensitively. The entire cost of this instrument does not exceed USD 760. In order to demonstrate the applicability of our PCR instrument, we conducted testing that revealed that we achieved gradient amplification and melting curves comparable to those of commercially available equipment. Good consistency characterized the testing outcomes. The successful detection of target genes demonstrates the reliability of our inexpensive PCR diagnostic technique. With this apparatus, there is no need to transport samples to a central laboratory; instead, we conduct testing at the sampling site. This saves time on transportation, substantially accelerates overall testing speed, and provides results within 40 min.
    Type of Medium: Online Resource
    ISSN: 2079-6374
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2662125-3
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  • 10
    Online Resource
    Online Resource
    MDPI AG ; 2022
    In:  Processes Vol. 10, No. 11 ( 2022-11-12), p. 2378-
    In: Processes, MDPI AG, Vol. 10, No. 11 ( 2022-11-12), p. 2378-
    Abstract: This paper investigates the pyrolysis of PODEn (n = 3, 4, 5) using ReaxFF molecular dynamics simulation. A large-scale model, which contains 2000 PODEn molecules, is simulated at 3000 K. The higher frequencies of the initial PODEn decomposition reaction at α or β C-O bond show that the α or β C-O bond in PODEn is not easy to break, which is approximately half the number of the other type of C-O bond dissociation. Furthermore, the bond dissociation energies (BDEs) are calculated using the ReaxFF method. The BDE of α or β C-O bond is higher than that of the other C-O bond, ~3–11 kcal/mol, indicating that BDE is one of the factors causing the different proportions of bonds broken. The evolution of pyrolysis products is also investigated. The results reveal that long-chain pyrolysis products from the initial PODEn decomposition are prone to further reaction, while a large amount of CH3O and CH3 remains in the system. This helps explain the difficulty in α and β C-O bond dissociation reactions. The results of the pyrolysis products are consistent with the result in further chemical kinetic simulation. The C2 species in pyrolysis products is relatively low, especially for C2H4 and C2H3, which is around zero. This supports the ability of PODEn to reduce soot emission.
    Type of Medium: Online Resource
    ISSN: 2227-9717
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2720994-5
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