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  • MDPI AG  (17)
  • 1
    In: Foods, MDPI AG, Vol. 11, No. 18 ( 2022-09-13), p. 2814-
    Kurzfassung: Ultrafine grinding is an important pretreatment to achieve the physical modification of dietary fiber. In this study, ultrafine grinding treatments were performed for different times to give pea insoluble dietary fiber (PIDF) samples with varied particle sizes (D50). The correlations and quantitative relationships between the microstructures of multi-scales PIDF and its in vitro glucose adsorption and diffusion behaviors were comprehensively evaluated. The results indicated that the specific surface area (SSA), pore volume (PV) and oxygen-to-carbon surface ratio (O/C) of PIDF were significantly increased by ultrafine grinding at the cellular scale, while D50 and cellulose crystallinity (CrI) were significantly decreased. These changes significantly improved the glucose adsorption capacity (GAC) of PIDF. The order of importance of microstructural changes on GAC was O/C 〉 PV 〉 SSA 〉 CrI 〉 D50. GAC showed positive exponential relationships with SSA, PV, and O/C and showed a negative linear relationship with CrI. The ability to retard glucose diffusion increased significantly with decreased fiber particle size because of improved adsorption and interception of glucose and the dense physical barrier effect of PIDF. The quantitative equation of maximum glucose dialysis retardation index was GDRImax = −1.65 ln(D50) + 16.82 ln(GAC) − 68.22 (R2 = 0.99). The results could provide theoretical support for quantitative and targeted intervention of dietary fiber structure for blood glucose control.
    Materialart: Online-Ressource
    ISSN: 2304-8158
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2022
    ZDB Id: 2704223-6
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    In: Sustainability, MDPI AG, Vol. 15, No. 1 ( 2022-12-30), p. 663-
    Kurzfassung: In recent decades, due to the intensification of human production and living activities, the process of soil acidification in China has been greatly accelerated, which has become an important factor limiting the sustainable development of agriculture. In this paper, an oyster shell soil conditioner prepared from discarded oyster shells was applied to the field and Shixia longan and chives were used as experimental objects for field experiments. Each crop was comprised of two groups. The application amount of longan in the control group was 0 kg/tree, and that in the experimental group was 8 kg/tree. The distribution of chives applied in the control group was 0 kg/m2, while that in the experimental group was 0.65 kg/m2. The results showed that, after the application of oyster shell soil conditioner, the soil pH value in Shixia longan experimental field increased by 1.30 units, and the content of soil organic matter, alkali hydrolyzed nitrogen and exchangeable calcium increased by 57.63%, 71.98%, and 49.13%. At the same time, the single fruit weight of Shixia longan increased by 6.37%, the soluble sugar content increased by 16.18%, and the titratable acid of the fruit decreased by 10.95%. Compared with the control group, the soil pH value of chives increased by 1.03 units, its yield increased by 57.8%, and various morphological indicators were improved. The results showed that the application of oyster shell soil conditioner could significantly improve the soil acidification of Shixia longan and chives, improve soil fertility, and effectively improve the yield and quality of fruits and vegetables.
    Materialart: Online-Ressource
    ISSN: 2071-1050
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2022
    ZDB Id: 2518383-7
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    In: Remote Sensing, MDPI AG, Vol. 15, No. 4 ( 2023-02-16), p. 1084-
    Kurzfassung: The rapid and accurate extraction of wide-area coseismic landslide locations is critical in earthquake emergencies. At present, the extraction of coseismic landslides is mainly based on post-earthquake site investigation or the interpretation of human–computer interactions based on remote sensing images. However, the identification efficiency is low, which seriously delays the earthquake emergency response. On the basis of the available multisource and multiscale remote sensing data, numerous studies have been carried out on the methods of coseismic landslide extraction, such as pixel analysis, object-oriented analysis, change detection, and machine learning. However, the effectiveness of coseismic landslide extraction was low in wide areas with complex topographic and geomorphic backgrounds. Therefore, this paper offers a comprehensive study of the factors influencing coseismic landslides and researches rapid and accurate wide-area coseismic landslide extraction methods with multisource remote sensing and geoscience technology. These techniques include digital elevation modeling (DEM) and its derived slopes and aspects. An embedded multichannel spectral–topographic feature fusion model for coseismic landslide extraction based on DeepLab V3+ is proposed, and a knowledge-enhanced deep learning information extraction method integrating geological knowledge is formed. Using the Jiuzhaigou Ms7.0 earthquake (seismic intensity VIII) in Sichuan Province, China, a comparison of landslide extraction models and strategies is carried out. The results show that the model proposed in this paper achieves the best balance in the accuracy and efficiency of wide-area extractions. Using multiple feature data of coseismic landslides, the problem of mixed pixels is solved. The rate of the misidentification of landslides as clouds, snow, buildings, and roads is significantly lower than in other methods. The identified landslide boundaries are smoother and more accurate, and the connectivity is better. Compared with other methods, ours can more accurately eliminate landslides not triggered by the Jiuzhaigou earthquake. While using the image block strategy to ensure extraction efficiency, it also improves the extraction accuracy of wide-area coseismic landslides in complex backgrounds.
    Materialart: Online-Ressource
    ISSN: 2072-4292
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2023
    ZDB Id: 2513863-7
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
    In: Molecules, MDPI AG, Vol. 28, No. 10 ( 2023-05-18), p. 4158-
    Kurzfassung: Tetrodotoxin (TTX) inhibits neurotransmission in animals, and there is no specific antidote. In clinical practice in China, Althaea rosea (A. rosea flower) extract has been used to treat TTX poisoning. In this work, the efficacy of the ethyl acetate fraction extract of A. rosea flower in treating TTX poisoning in rats was investigated. A high-performance liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was developed to determine nine neurotransmitters in rat brain tissue, including γ-aminobutyric acid (GABA), dopamine (DA), 5-hydroxytryptamine (5-HT), noradrenaline (NE), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 5-hydroxyindole-3-acetic acid (5-HIAA), epinephrine (E), and tyramine (Tyn). The detoxifying effect of A. rosea flower was verified by comparing the changes in neurotransmitters’ content in brain tissue before and after poisoning in rats. The assay was performed in multiple reaction monitoring mode. The quantification method was performed by plotting an internal-standard working curve with good linearity (R2 〉 0.9941) and sensitivity. Analyte recoveries were 94.04–107.53% (RSD 〈 4.21%). Results indicated that the levels of 5-HT, DA, E, and NE in the brains of TTX-intoxicated rats decreased, whereas the levels of GABA, Tyn, and 5-HIAA showed an opposite trend, and HVA and DOPAC were not detected. The levels of all seven neurotransmitters returned to normal after the gavage administration of ethyl acetate extract of A. rosea flower to prove that the ethyl acetate extract of A. rosea flower had a therapeutic effect on TTX poisoning. The work provided new ideas for studies on TTX detoxification.
    Materialart: Online-Ressource
    ISSN: 1420-3049
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2023
    ZDB Id: 2008644-1
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 5
    Online-Ressource
    Online-Ressource
    MDPI AG ; 2022
    In:  Buildings Vol. 12, No. 10 ( 2022-10-17), p. 1709-
    In: Buildings, MDPI AG, Vol. 12, No. 10 ( 2022-10-17), p. 1709-
    Kurzfassung: Blockchain has great potential for facilitating the development of the construction industry but has not been widely used to this end. The objective of this study was to identify the factors affecting the adoption of blockchain in the construction industry from the technical, organizational, and environmental dimensions with the help of theories related to technology adoption. Empirical results showed that relative advantage, compatibility, competitive pressure, technological maturity, organizational readiness, and policy have an impact on intention to adopt blockchain in the construction industry through perceived usefulness or perceived ease of use. Competitive pressure has the greatest impact on the internal variables of the technology acceptance model (TAM) (0.696). Perceived cost of adoption does not have a significant effect on blockchain adoption behavior. However, in contrast to previous research, organizational readiness has a negative effect (−0.03) on perceived usefulness. The research results provide inspiration for further research on the impact mechanisms of blockchain adoption in the construction industry, as well as guidance for governments to formulate blockchain adoption policies and guidance for the widespread application of blockchain in construction.
    Materialart: Online-Ressource
    ISSN: 2075-5309
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2022
    ZDB Id: 2661539-3
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 6
    Online-Ressource
    Online-Ressource
    MDPI AG ; 2016
    In:  Sensors Vol. 16, No. 10 ( 2016-09-22), p. 1562-
    In: Sensors, MDPI AG, Vol. 16, No. 10 ( 2016-09-22), p. 1562-
    Materialart: Online-Ressource
    ISSN: 1424-8220
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2016
    ZDB Id: 2052857-7
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 7
    In: Energies, MDPI AG, Vol. 14, No. 10 ( 2021-05-12), p. 2782-
    Kurzfassung: The Chang 7 continental shale oil reservoir is tight. The recovery factor is extremely low, the remaining oil is very high, and injecting water to improve oil recovery effectiveness is too hard. Therefore, in this paper, physical simulation experiments of CO2 huff-n-puff shale oil and NMR tests were conducted to study the cycle numbers and permeability on the recovery degree, as well as the characteristics of shale oil mobilization and the remaining oil micro distribution. The results showed that the cumulative oil recovery factors (ORFs) gradually increased in the natural logarithmic form, the single cycle ORFs decreased rapidly in exponential form with the huff-n-puff cycle number, and the biggest economic cycle numbers were between approximately 3 and 5. Furthermore, the higher the permeability, the higher the ORF, but the difference of ORF decreased between the two experimental samples with the cycles. In addition, the gap of production and recovery degree was large between the different scale pores, the ORF of macropores was 6–8 times that of micropores, and the final remaining oil was mainly distributed in the micropores, accounting for 82.29% of the total amount; meanwhile, the macropores comprised less than 0.5%. In the process of huff-n-puff, CO2 flowed into macropores, mesopores, and smallpores under the pressure differential effect, but a small amount of CO2 slowly diffused into micropores, resulting in the ORF of the former with more free oil being higher and the ORF of micropores with more adsorbed oil being lower. Therefore, promoting a better contact and reaction between CO2 and shale oil of micropores is one of the key ways to effectively develop the Chang 7 continental shale oil and enhance oil recovery.
    Materialart: Online-Ressource
    ISSN: 1996-1073
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2021
    ZDB Id: 2437446-5
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 8
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 23, No. 4 ( 2022-02-14), p. 2113-
    Kurzfassung: Bacterial leaf pustule (BLP), caused by Xanthornonas axonopodis pv. glycines (Xag), is a worldwide disease of soybean, particularly in warm and humid regions. To date, little is known about the underlying molecular mechanisms of BLP resistance. The only single recessive resistance gene rxp has not been functionally identified yet, even though the genotypes carrying the gene have been widely used for BLP resistance breeding. Using a linkage mapping in a recombinant inbred line (RIL) population against the Xag strain Chinese C5, we identified that quantitative trait locus (QTL) qrxp–17–2 accounted for 74.33% of the total phenotypic variations. We also identified two minor QTLs, qrxp–05–1 and qrxp–17–1, that accounted for 7.26% and 22.26% of the total phenotypic variations, respectively, for the first time. Using a genome-wide association study (GWAS) in 476 cultivars of a soybean breeding germplasm population, we identified a total of 38 quantitative trait nucleotides (QTNs) on chromosomes (Chr) 5, 7, 8, 9,15, 17, 19, and 20 under artificial infection with C5, and 34 QTNs on Chr 4, 5, 6, 9, 13, 16, 17, 18, and 20 under natural morbidity condition. Taken together, three QTLs and 11 stable QTNs were detected in both linkage mapping and GWAS analysis, and located in three genomic regions with the major genomic region containing qrxp_17_2. Real-time RT-PCR analysis of the relative expression levels of five potential candidate genes in the resistant soybean cultivar W82 following Xag treatment showed that of Glyma.17G086300, which is located in qrxp–17–2, significantly increased in W82 at 24 and 72 h post-inoculation (hpi) when compared to that in the susceptible cultivar Jack. These results indicate that Glyma.17G086300 is a potential candidate gene for rxp and the QTLs and QTNs identified in this study will be useful for marker development for the breeding of Xag-resistant soybean cultivars.
    Materialart: Online-Ressource
    ISSN: 1422-0067
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2022
    ZDB Id: 2019364-6
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 9
    Online-Ressource
    Online-Ressource
    MDPI AG ; 2019
    In:  International Journal of Molecular Sciences Vol. 20, No. 7 ( 2019-03-27), p. 1533-
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 20, No. 7 ( 2019-03-27), p. 1533-
    Kurzfassung: Methoxylated coumarins represent a large proportion of officinal value coumarins while only one enzyme specific to bergaptol O-methylation (BMT) has been identified to date. The multiple types of methoxylated coumarins indicate that at least one unknown enzyme participates in the O-methylation of other hydroxylated coumarins and remains to be identified. Combined transcriptome and metabonomics analysis revealed that an enzyme similar to caffeic acid O-methyltransferase (COMT-S, S is short for similar) was involved in catalyzing all the hydroxylated coumarins in Peucedanum praeruptorum. However, the precise molecular mechanism of its substrate heterozygosis remains unsolved. Pursuing this question, we determined the crystal structure of COMT-S to clarify its substrate preference. The result revealed that Asn132, Asp271, and Asn325 govern the substrate heterozygosis of COMT-S. A single mutation, such as N132A, determines the catalytic selectivity of hydroxyl groups in esculetin and also causes production differences in bergapten. Evolution-based analysis indicated that BMT was only recently derived as a paralogue of caffeic acid O-methyltransferase (COMT) via gene duplication, occurring before the Apiaceae family divergence between 37 and 100 mya. The present study identified the previously unknown O-methylation steps in coumarin biosynthesis. The crystallographic and mutational studies provided a deeper understanding of the substrate preference, which can be used for producing specific O-methylation coumarins. Moreover, the evolutionary relationship between BMT and COMT-S was clarified to facilitate understanding of evolutionary events in the Apiaceae family.
    Materialart: Online-Ressource
    ISSN: 1422-0067
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2019
    ZDB Id: 2019364-6
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 10
    In: Foods, MDPI AG, Vol. 12, No. 14 ( 2023-07-20), p. 2764-
    Kurzfassung: Halosulfuron-methyl (HM) is widely used for the removal of noxious weeds in corn, sugarcane, wheat, rice, and tomato fields. Despite its high efficiency and low toxicity, drift to nontarget crops and leaching of its metabolites to groundwater pose potential risks. Considering the instability of HM, the pyrazole sulfonamide of HM was used to generate a hapten and antigen to raise a high-quality monoclonal antibody (Mab, designated 1A91H11) against HM. A direct competitive immunoassay (dcELISA) using Mab 1A91H11 achieved a half-maximal inhibitory concentration (IC50) of 1.5 × 10−3 mg/kg and a linear range of 0.7 × 10−3 mg/kg–10.7 × 10−3 mg/kg, which was 10 times more sensitive than a comparable indirect competitive ELISA (icELISA) and more simple to operate. A spiking recovery experiment performed in tomato and maize matrices with 0.01, 0.05, and 0.1 mg/kg HM had average recoveries within 78.9–87.9% and 103.0–107.4% and coefficients of variation from 1.1–6.8% and 2.7–6.4% in tomato and maize, respectively. In addition, a magnetic lateral flow immunoassay (MLFIA) was developed for quantitative detection of low concentrations of HM in paddy water. Compared with dcELISA, the MLFIA exhibited 3.3- to 50-fold higher sensitivity (IC50 0.21 × 10−3 mg/kg). The average recovery and RSD of the developed MLFIA ranged from 81.5 to 92.5% and 5.4 to 9.7%. The results of this study demonstrated that the developed dcELISA and MLFIA are suitable for rapid detection of HM residues in tomato and maize matrices and paddy water, respectively, with acceptable accuracy and precision.
    Materialart: Online-Ressource
    ISSN: 2304-8158
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2023
    ZDB Id: 2704223-6
    Standort Signatur Einschränkungen Verfügbarkeit
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