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  • MDPI AG  (27)
  • 1
    In: Coatings, MDPI AG, Vol. 12, No. 3 ( 2022-03-19), p. 410-
    Abstract: Jiatang scroll paintings listed in the Chinese intangible cultural heritage list are an important form of family trees. In this paper, a Jiatang scroll painting drawn in the seventh year of Guangxu (AD 1881) was chosen as a prototype to analyze its components. Samples were taken from different parts of the Jiatang scroll painting to analyze the composition of fibers, pigments, and adhesives. Herein, fiber analyzer, Fourier transform infrared spectroscopy (FTIR), and Herzberg stain were conducted to identify the type of fiber used. Microstructural and microchemical analyses by means of SEM-EDX and Raman spectroscopy were performed to characterize the pigments present in the painting, while the nature of the adhesive was examined using pyrolysis gas chromatography–mass spectrometry (Py-GC/MS). The results show that the painting fiber is from cotton, while the red, black, and green pigments were identified as cinnabar, carbon black, and emerald green, respectively. Finally, the adhesive is a mixture of animal glue, benzoin gum, and starch. The research results provide a basis for the protection and restoration of this precious cultural heritage in the future.
    Type of Medium: Online Resource
    ISSN: 2079-6412
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2662314-6
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  • 2
    In: Animals, MDPI AG, Vol. 12, No. 13 ( 2022-06-26), p. 1644-
    Abstract: N6-methyladenosine (m6A) modification can accommodate mRNA processing, stability, and translation in mammals, and fat mass and obesity associated protein (FTO) is a vital demethylase in the m6A modification pathway. Clostridium perfringens type C (C. perfringens type C) causes diarrhea in piglets and has a serious impact on the pig industry. However, our understanding of the effect of m6A in the process of C. perfringens type C infectious piglet diarrhea (CPTCIPD) is limited. Here, an in vitro model of CPTCIPD was constructed by treating the intestinal porcine epithelial cell line-J2 (IPEC-J2) with Clostridium perfringens beta2 (CPB2) toxin, and the role of FTO was analyzed using quantitative real-time polymerase chain reaction, Western blotting, and flow cytometry. The results revealed that the overall RNA m6A contents at the tissue and cell levels were significantly up-regulated after C. perfringens infection (p 〈 0.05). FTO expression was significantly reduced in CPB2-treated IPEC-J2 cells. Functionally, FTO knockdown in the treated cells inhibited their proliferation and promoted apoptosis and the inflammation phenotype, whereas FTO overexpression had the opposite effects. Inhibiting FTO prolonged the half-life and up-regulated the expression of Caspase 3, leading to apoptosis. Therefore, this work explored the regulation of FTO in IPEC-J2 cells after CPB2 treatment and enhanced our understanding of the effect of the m6A modification in CPTCIPD.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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  • 3
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 23, No. 24 ( 2022-12-13), p. 15833-
    Abstract: Clostridium perfringens beta2 (CPB2) toxin is one of the main pathogenic toxins produced by Clostridium perfringens, which causes intestinal diseases in animals and humans. The N6-methyladenosine (m6A) modification is the most common reversible modification in eukaryotic disease processes. Methyltransferase-like 3 (METTL3) regulates immunity and inflammatory responses induced by the bacterial infections in animals. However, METTL3′s involvement in CPB2-treated intestinal porcine epithelial cell line-J2 (IPEC-J2) remains unclear. In the current study, we used methylated RNA immunoprecipitation-quantitative polymerase chain reaction, Western blotting and immunofluorescence assay to determine the role of METTL3 in CPB2-exposed IPEC-J2 cells. The findings revealed that m6A and METTL3 levels were increased in CPB2 treated IPEC-J2 cells. Functionally, METTL3 overexpression promoted the release of inflammatory factors, increased cytotoxicity, decreased cell viability and disrupted tight junctions between cells, while the knockdown of METTL3 reversed these results. Furthermore, METTL3 was involved in the inflammatory response of IPEC-J2 cells by activating the TLR2/NF-κB signaling pathway through regulating TLR2 m6A levels. In conclusion, METTL3 overexpression triggered the TLR2/NF-κB signaling pathway and promoted CPB2-induced inflammatory responses in IPEC-J2 cells. These findings may provide a new strategy for the prevention and treatment of diarrhea caused by Clostridium perfringens.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    MDPI AG ; 2023
    In:  Nutrients Vol. 15, No. 7 ( 2023-03-23), p. 1564-
    In: Nutrients, MDPI AG, Vol. 15, No. 7 ( 2023-03-23), p. 1564-
    Abstract: Wheat allergy is a primary disease of food allergy, and its global prevalence is unclear. This study aimed to characterize the latest worldwide prevalence of wheat allergy based on five different diagnostic methods. Study searches were conducted in Web of Science, PubMed, Ovid LWW, and Cochrane database, with a time limit of 1 January 2007 to 1 September 2022. The review and screening of the articles was undertaken by two independent reviewers. The statistical analysis was conducted by R. A total of 56 articles were finally included. The prevalence of wheat allergy was 0.63% (95% CI: 0.43–0.87%) for self-reported, 0.70% (95% CI: 0.18–1.22%) for self-reported physician-diagnosed, 0.22% (95%CI: 0.07–0.65%) for skin prick test positive, 0.97% (95% CI: 0.43–2.20%) for specific immunoglobulin E positive, and 0.04% (95% CI: 0–0.16%) for food challenge. However, food challenge can be largely subjective, and the results were only based two countries, so the prevalence of wheat allergy confirmed by food challenge may be not entirely trustworthy. In conclusion, investigating the prevalence of wheat allergy in the real world as accurately as possible will contribute to the prevention, management, and risk assessment of wheat allergy.
    Type of Medium: Online Resource
    ISSN: 2072-6643
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2518386-2
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  • 5
    In: Water, MDPI AG, Vol. 11, No. 4 ( 2019-04-17), p. 793-
    Abstract: Artemisia halodendron is a widely distributed native plant in China’s Horqin sandy land, but few studies have examined its physiological responses to drought and rehydration. To provide more information, we investigated the effects of drought and rehydration on the chlorophyll fluorescence parameters and physiological responses of A. halodendron to reveal the mechanisms responsible for A. halodendron’s tolerance of drought stress and the resulting ability to tolerate drought. We found that A. halodendron had strong drought resistance. Its chlorophyll content first increased and then decreased with prolonged drought. Variable chlorophyll fluorescence (Fv) and quantum efficiency of photosystem II (Fv/Fm) decreased, and the membrane permeability and malondialdehyde increased. When plants were subjected to drought stress, superoxide dismutase (SOD) activity degraded under severe drought, but the activities of peroxidase (POD) and catalase (CAT) and the contents of soluble proteins, soluble sugars, and free proline increased. Severe drought caused wilting of A. halodendron leaves and the leaves failed to recover even after rehydration. After rehydration, the chlorophyll content, membrane permeability, SOD and CAT activities, and the contents of the three osmoregulatory substances under moderate drought began to recover. However, Fv, Fv/Fm, malondialdehyde, and POD activity did not recover under severe drought. These results illustrated that drought tolerance of A. halodendron resulted from increased enzyme (POD and CAT) activities and accumulation of osmoregulatory substances.
    Type of Medium: Online Resource
    ISSN: 2073-4441
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2521238-2
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  • 6
    In: Molecules, MDPI AG, Vol. 27, No. 24 ( 2022-12-13), p. 8844-
    Abstract: Colorectal cancer (CRC) is one of the most common causes of cancer-related death worldwide, and more therapies are needed to treat CRC. To discover novel CRC chemotherapeutic molecules, we used a series of previously synthesized novel imidazolidin-4-one derivatives to study their anticancer role in several cancer cell lines. Among these compounds, compound 9r exhibited the best anticancer activity in CRC cell lines HCT116 and SW620. We further investigated the anticancer molecular mechanism of compound 9r. We found that compound 9r induced mitochondrial pathway apoptosis in HCT116 and SW620 cells by inducing reactive oxygen species (ROS) production. Moreover, the elevated ROS generation activated the c-Jun N-terminal kinase (JNK) pathway, which further accelerated apoptosis. N-acetylcysteine (NAC), an antioxidant reagent, suppressed compound 9r-induced ROS production, JNK pathway activation, and apoptosis. Collectively, this research synthesized a series of imidazolidin-4-one derivatives, evaluated their anticancer activity, and explored the molecular mechanism of compound 9r-induced apoptosis in CRC cells. The present results suggest that compound 9r has a potential therapeutic role in CRC. Hence, it deserves further exploration as a lead compound for CRC treatment.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2008644-1
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  • 7
    Online Resource
    Online Resource
    MDPI AG ; 2021
    In:  International Journal of Molecular Sciences Vol. 22, No. 22 ( 2021-11-09), p. 12125-
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 22, No. 22 ( 2021-11-09), p. 12125-
    Abstract: The Chinese medicine monomer cynaroside (Cy) is a flavonoid glycoside compound that widely exists in plants and has a variety of pharmacological effects, such as its important role in the respiratory system, cardiovascular system and central nervous system. Studies have reported that Cy has varying degrees of anticancer activity in non-small cell lung cancer, cervical cancer, liver cancer, esophageal cancer and other cancers. However, there are no relevant reports about its role in gastric cancer. The MET/AKT/mTOR signaling pathway plays important roles in regulating various biological processes, including cell proliferation, apoptosis, autophagy, invasion and tumorigenesis. In this study, we confirmed that Cy can inhibit the cell growth, migration and invasion and tumorigenesis in gastric cancer. Our finding shows that Cy can block the MET/AKT/mTOR axis by decreasing the phosphorylation level of AKT, mTOR and P70S6K. Therefore, the MET/AKT/mTOR axis may be an important target for Cy. In summary, Cy has anti-cancer properties and is expected to be a potential drug for the treatment of gastric cancer.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 8
    Online Resource
    Online Resource
    MDPI AG ; 2019
    In:  Water Vol. 11, No. 6 ( 2019-06-05), p. 1181-
    In: Water, MDPI AG, Vol. 11, No. 6 ( 2019-06-05), p. 1181-
    Abstract: Bridge piers on river channels can cause obstacles for flood flow by reducing the cross-sectional area and inducing local eddy currents and high flow velocities, which may destroy hydraulic structures. A two-dimensional numerical model was used to investigate the effects of bridge piers on river flood hazards in the Jialing River, China. For the modeling, Mike 21 FM was used, which is an unstructured mesh and finite volume model that solves the shallow water equations. The numerical model was validated with collected historical flood traces, and sensitivity analyses identified the effects of the Manning coefficient and the dependence on the grid size. The influence of backwater effects on the flow field was analyzed by comparing numerical results with and without piers. The results showed that the most significant impacts were caused by the Fengxian Bridge. The maximum water level rise was about 1 m and the maximum velocity near this bridge decreased by 22.77% for a 10-year flood. We found that the top elevations of planned levees near the bridges must be increased by 0.15–0.36 m. The influence of bridge piers on the flood velocity field is more complex. These findings will help flood hazard management in this river and provides a reference for similar projects.
    Type of Medium: Online Resource
    ISSN: 2073-4441
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2521238-2
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  • 9
    In: Coatings, MDPI AG, Vol. 12, No. 9 ( 2022-08-31), p. 1269-
    Abstract: Excavated human bones are important materials for revealing information about ancient human food, the ancient environment and the ancient climate, and the origins of ancient humans. Often, the chemical composition and biological characteristics of bones have changed to varying degrees, which means that they are contaminated and cannot be used for further analysis. Through research on the correlation between human bones excavated from a burial environment and their state of preservation, judging whether the excavated bones are contaminated is a prerequisite for scientific analysis, environmental archeology, and research on ancient human recipes. In this paper, human bones excavated from the Shenna ruins and the contamination of them in the burial environment has been judged using craniofacial measurement characteristics, pH measurement, scanning electron microscopy–energy dispersive spectrometer (SEM-EDS), X-ray diffraction (XRD), Fourier transform red external light spectrum (FTIR), and stable isotope tests (SIT). However, the organic compositions of the remains at Shenna are decomposed to a large degree, and the inorganic structure has been destroyed, which will eventually cause looseness and porosity, and the compositions of inorganic minerals in the human bones have not been changed or contaminated. The results indicate that the burial environment at Shenna accelerated the deterioration of human bones, but it has not affected the typical character of the human bone, and the human bones extracted can still be used for further trace element testing. Moreover, this can help to reduce the effort required to reveal information about ancient human food, as well as the need for further studies of the paleoenvironment and paleoclimate in the Shenna ruins.
    Type of Medium: Online Resource
    ISSN: 2079-6412
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2662314-6
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  • 10
    In: Animals, MDPI AG, Vol. 11, No. 8 ( 2021-07-30), p. 2263-
    Abstract: Chinese HZ boars are typical plateau miniature boars characterized by precocious puberty, which is closely related to testicular development and spermatogenesis. Accumulating evidence indicates that lncRNA is involved in the testicular development and regulation of spermatogenesis. However, little is known about the lncRNA precocious regulation in testicular development and spermatogenesis on early sexual maturity of HZ boars. Thus, we investigated the expression and characterization of lncRNA and mRNA in 30-day-old and 120-day-old HZ boar testes using transcriptome to explore precocious puberty. Landrace (LC) boar was treated as the control. Histological analyses indicated that HZ boar underwent puberty development at an earlier stage than LC boar and had achieved sexual maturity at 120 days old. RNA-Seq yielded a total of 187 lncRNAs and 984 mRNAs; these molecules were identified as possible candidates for precocious puberty. GO terms and KEGG pathways enrichment analyses revealed that the differentially expressed lncRNA and their targeted genes were involved in metabolic pathways regulating testis development and spermatogenesis, such as the PI3K-Akt, TGF-beta and Wnt pathways. Further screening, some lncRNA (such as LOC102166140, LOC110259451, and MSTRG.15011.2), and mRNA (such as PDCL2, HSD17B4, SHCBP1L, CYP21A2, and SPATA3) were found to be possibly associated with precocious puberty, which would add to our understanding of the molecular regulatory mechanisms of precocious puberty. This study provided valuable information for further study of the role of lncRNA and mRNA in the process of precocious puberty.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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