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  • 1
    In: Materials, MDPI AG, Vol. 16, No. 6 ( 2023-03-15), p. 2349-
    Abstract: An optimization design of the bending-vibration resistance of magnetorheological elastomer carbon fibre reinforced polymer sandwich sheets (MECFRPSSs) was studied in this paper. Initially, by adopting the classical laminate theory, the Reddy’s high-order shear deformation theory, the Rayleigh-Ritz method, etc., an analytical model of the MECFRPSSs was established to predict both bending and vibration parameters, with the three-point bending forces and a pulse load being considered separately. After the validation of the model was completed, the optimization design work of the MECFRPSSs was conducted based on an optimization model developed, in which the thickness, modulus, and density ratios of magnetorheological elastomer core to carbon fibre reinforced polymer were taken as design variables, and static bending stiffness, the averaged damping, and dynamic stiffness parameters were chosen as objective functions. Subsequently, an artificial bee colony algorithm was adopted to execute single-objective, dual-objective, and multi-objective optimizations to obtain the optimal design parameters of such structures, with the convergence effectiveness being examined in a validation example. It was found that it was hard to improve the bending, damping, and dynamic stiffness behaviours of the structure simultaneously as the values of design variables increased. Some compromised results of design parameters need to be determined, which are based on Pareto-optimal solutions. In further engineering application of the MECFRPSSs, it is suggested to use the corresponding design parameters related to a turning point to better exert their bending-vibration resistance.
    Type of Medium: Online Resource
    ISSN: 1996-1944
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2487261-1
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  • 2
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 13, No. 4 ( 2012-04-10), p. 4545-4563
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2012
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    MDPI AG ; 2023
    In:  Agronomy Vol. 13, No. 7 ( 2023-07-08), p. 1816-
    In: Agronomy, MDPI AG, Vol. 13, No. 7 ( 2023-07-08), p. 1816-
    Abstract: The vision-based fruit recognition and localization system is the basis for the automatic operation of agricultural harvesting robots. Existing detection models are often constrained by high complexity and slow inference speed, which do not meet the real-time requirements of harvesting robots. Here, a method for apple object detection and localization is proposed to address the above problems. First, an improved YOLOX network is designed to detect the target region, with a multi-branch topology in the training phase and a single-branch structure in the inference phase. The spatial pyramid pooling layer (SPP) with serial structure is used to expand the receptive field of the backbone network and ensure a fixed output. Second, the RGB-D camera is used to obtain the aligned depth image and to calculate the depth value of the desired point. Finally, the three-dimensional coordinates of apple-picking points are obtained by combining two-dimensional coordinates in the RGB image and depth value. Experimental results show that the proposed method has high accuracy and real-time performance: F1 is 93%, mean average precision (mAP) is 94.09%, detection speed can reach 167.43 F/s, and the positioning errors in X, Y, and Z directions are less than 7 mm, 7 mm, and 5 mm, respectively.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2607043-1
    SSG: 23
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  • 4
    In: Cancers, MDPI AG, Vol. 12, No. 8 ( 2020-08-08), p. 2221-
    Abstract: RNA-binding protein Musashi-1 (MSI1) is a key regulator of several stem cell populations. MSI1 is involved in tumor proliferation and maintenance, and it regulates target mRNAs at the translational level. The known mRNA targets of MSI1 include Numb, APC, and P21WAF-1, key regulators of Notch/Wnt signaling and cell cycle progression, respectively. In this study, we aim to identify small molecule inhibitors of MSI1–mRNA interactions, which could block the growth of cancer cells with high levels of MSI1. Using a fluorescence polarization (FP) assay, we screened small molecules from several chemical libraries for those that disrupt the binding of MSI1 to its consensus RNA. One cluster of hit compounds is the derivatives of secondary metabolites from Aspergillus nidulans. One of the top hits, Aza-9, from this cluster was further validated by surface plasmon resonance and nuclear magnetic resonance spectroscopy, which demonstrated that Aza-9 binds directly to MSI1, and the binding is at the RNA binding pocket. We also show that Aza-9 binds to Musashi-2 (MSI2) as well. To test whether Aza-9 has anti-cancer potential, we used liposomes to facilitate Aza-9 cellular uptake. Aza-9-liposome inhibits proliferation, induces apoptosis and autophagy, and down-regulates Notch and Wnt signaling in colon cancer cell lines. In conclusion, we identified a series of potential lead compounds for inhibiting MSI1/2 function, while establishing a framework for identifying small molecule inhibitors of RNA binding proteins using FP-based screening methodology.
    Type of Medium: Online Resource
    ISSN: 2072-6694
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2527080-1
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  • 5
    In: Energies, MDPI AG, Vol. 15, No. 11 ( 2022-05-28), p. 3989-
    Abstract: Two comb-type copolymers were synthesized by modifications of the maleic anhydride-α-octadecene copolymer with aniline (AMAC) and with phenethylamine (EMAC), respectively. The effects of AMAC and EMAC on the non-isothermal crystallization kinetics of the MEK–toluene dewaxing process were comparatively investigated. The results showed that, under the optimum process conditions, the addition of 100 ppm AMAC or EMAC could increase the yield of dewaxed oil (DWO) from 67.21% to 70.87% or 69.90%, respectively, and increase the filtration rate by 80% and 70%, respectively. The non-isothermal crystallization kinetics analysis showed that the crystallization process conformed to Mo’s equation, and the addition of AMAC and EMAC slowed down the crystallization rate of paraffin. The polarizing light microscope observations revealed that the addition of AMAC promoted the formation of snow flower-like crystals, and the EMAC addition led to the crystals having longer rods with a relatively complete morphology. This work provided useful experimental data for a better understanding of the roles of comb-type copolymer additives in the MEK–toluene dewaxing process.
    Type of Medium: Online Resource
    ISSN: 1996-1073
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2437446-5
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  • 6
    Online Resource
    Online Resource
    MDPI AG ; 2023
    In:  Remote Sensing Vol. 15, No. 10 ( 2023-05-11), p. 2530-
    In: Remote Sensing, MDPI AG, Vol. 15, No. 10 ( 2023-05-11), p. 2530-
    Abstract: The acquisition of maize tassel phenotype information plays a vital role in studying maize growth and improving yield. Unfortunately, detecting maize tassels has proven challenging because of the complex field environment, including image resolution, varying sunlight conditions, plant varieties, and planting density. To address this situation, the present study uses unmanned aerial vehicle (UAV) remote sensing technology and a deep learning algorithm to facilitate maize tassel identification and counting. UAVs are used to collect maize tassel images in experimental fields, and RetinaNet serves as the basic model for detecting maize tassels. Small maize tassels are accurately identified by optimizing the feature pyramid structure in the model and introducing attention mechanisms. We also study how mapping differences in image resolution, brightness, plant variety, and planting density affect the RetinaNet model. The results show that the improved RetinaNet model is significantly better at detecting maize tassels than the original RetinaNet model. The average precision in this study is 0.9717, the precision is 0.9802, and the recall rate is 0.9036. Compared with the original model, the improved RetinaNet improves the average precision, precision, and recall rate by 1.84%, 1.57%, and 4.6%, respectively. Compared with mainstream target detection models such as Faster R-CNN, YOLOX, and SSD, the improved RetinaNet model more accurately detects smaller maize tassels. For equal-area images of differing resolution, maize tassel detection becomes progressively worse as the resolution decreases. We also analyze how detection depends on brightness in the various models. With increasing image brightness, the maize tassel detection worsens, especially for small maize tassels. This paper also analyzes the various models for detecting the tassels of five maize varieties. Zhengdan958 tassels prove the easiest to detect, with R2 = 0.9708, 0.9759, and 0.9545 on 5, 9, and 20 August 2021, respectively. Finally, we use the various models to detect maize tassels under different planting densities. At 29,985, 44,978, 67,466, and 89,955 plants/hm2, the mean absolute errors for detecting Zhengdan958 tassels are 0.18, 0.26, 0.48, and 0.63, respectively. Thus, the detection error increases gradually with increasing planting density. This study thus provides a new method for high-precision identification of maize tassels in farmland and is especially useful for detecting small maize tassels. This technology can be used for high-throughput investigations of maize phenotypic traits.
    Type of Medium: Online Resource
    ISSN: 2072-4292
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2513863-7
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  • 7
    In: Micromachines, MDPI AG, Vol. 11, No. 12 ( 2020-11-26), p. 1037-
    Abstract: Cell separation plays an important role in the fields of analytical chemistry and biomedicine. To solve the blockage problem and improve the separation throughput in the traditional microstructure filtration-based separation approach, a continuous cell separation and collection approach via micropost array railing on a microfilter and negative dielectrophoresis combined chip is proposed. By tilting the micropost array at a certain angle, microparticles or cells enter the collection area under micropost array railing. The effects of the inclination angle of the micropost array and the electrode distance on the microparticle collection efficiency were investigated. Based on the optimized microfluidic chip structure, 37- and 16.3-μm particles were collected with 85% and 89% efficiencies, respectively. Additionally, algal cells were separated and collected by using the optimized microchip. The chip also had good separation and collection effects on biological samples, which effectively solved the blockage problem and improved the separation throughput, laying a foundation for subsequent microstructure filtration separation-based research and application.
    Type of Medium: Online Resource
    ISSN: 2072-666X
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2620864-7
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  • 8
    In: Journal of Marine Science and Engineering, MDPI AG, Vol. 10, No. 3 ( 2022-03-16), p. 434-
    Abstract: The stratigraphic-diffusive type of gas hydrate system is formed by microbial methane produced in a shallow slope space when flowing laterally into hydrate stable zones and is worth studying for both energy supply and academic understanding. A deposition production model matching the vertical and lateral seabed morphological characteristics was constructed to show the accumulation process, layer timing sequence, and reservoir quality of the stratigraphic-diffusive hydrate system in the Dongsha slope sediments since the Pleistocene. Six representative key system factors at three selected moments (1.5 Ma, 700 ka B.P., and at present) have been exhibited during debris is continuously accumulating. The coexistence of the hydrate decomposition in the lower part and the formation in the upper part, and the uneven distribution of hydrates within the slope sediment surface are explained clearly. By comparing four geological cases with diverse environments, it is shown that the diffusive hydrate system is likely to develop into moderate geological conditions. The most powerful carbon fixation ability in this system was quantified within the time range of 100−50 ka B.P. Finally, it was verified that residual methane would converge near the seafloor interface and then eventually overflow out of the seabed into the seawater.
    Type of Medium: Online Resource
    ISSN: 2077-1312
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2738390-8
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  • 9
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 24, No. 7 ( 2023-03-23), p. 6066-
    Abstract: To investigate the role of peroxisome proliferator-activated receptor alpha (PPARα) in carnitine status and intestinal fatty acid oxidation in neonates, a total of 72 suckled newborn piglets were assigned into 8 dietary treatments following a 2 (±0.35% clofibrate) × 4 (diets with: succinate+glycerol (Succ), tri-valerate (TC5), tri-hexanoate (TC6), or tri-2-methylpentanoate (TMPA)) factorial design. All pigs received experimental milk diets with isocaloric energy for 5 days. Carnitine statuses were evaluated, and fatty acid oxidation was measured in vitro using [1-14C]-palmitic acid (1 mM) as a substrate in absence or presence of L659699 (1.6 µM), iodoacetamide (50 µM), and carnitine (1 mM). Clofibrate increased concentrations of free (41%) and/or acyl-carnitine (44% and 15%) in liver and plasma but had no effects in the intestine. The effects on carnitine status were associated with the expression of genes involved in carnitine biosynthesis, absorption, and transportation. TC5 and TMPA stimulated the increased fatty acid oxidation rate induced by clofibrate, while TC6 had no effect on the increased fatty acid oxidation induced by clofibrate (p 〉 0.05). These results suggest that dietary clofibrate improved carnitine status and increased fatty acid oxidation. Propionyl-CoA, generated from TC5 and TMPA, could stimulate the increased fatty acid oxidation rate induced by clofibrate as anaplerotic carbon sources.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 10
    In: Remote Sensing, MDPI AG, Vol. 6, No. 11 ( 2014-11-19), p. 11494-11517
    Type of Medium: Online Resource
    ISSN: 2072-4292
    Language: English
    Publisher: MDPI AG
    Publication Date: 2014
    detail.hit.zdb_id: 2513863-7
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