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  • MDPI AG  (191)
  • 1
    In: Remote Sensing, MDPI AG, Vol. 13, No. 24 ( 2021-12-16), p. 5122-
    Abstract: This project explored the integrated use of satellite, ground observations and hydrological distributed models to support water resources assessment and monitoring in High Mountain Asia (HMA). Hydrological data products were generated taking advantage of the synergies of European and Chinese data assets and space-borne observation systems. Energy-budget-based glacier mass balance and hydrological models driven by satellite observations were developed. These models can be applied to describe glacier-melt contribution to river flow. Satellite hydrological data products were used for forcing, calibration, validation and data assimilation in distributed river basin models. A pilot study was carried out on the Red River basin. Multiple hydrological data products were generated using the data collected by Chinese satellites. A new Evapo-Transpiration (ET) dataset from 2000 to 2018 was generated, including plant transpiration, soil evaporation, rainfall interception loss, snow/ice sublimation and open water evaporation. Higher resolution data were used to characterize glaciers and their response to environmental forcing. These studies focused on the Parlung Zangbo Basin, where glacier facies were mapped with GaoFeng (GF), Sentinal-2/Multi-Spectral Imager (S2/MSI) and Landsat8/Operational Land Imager (L8/OLI) data. The geodetic mass balance was estimated between 2000 and 2017 with Zi-Yuan (ZY)-3 Stereo Images and the SRTM DEM. Surface velocity was studied with Landsat5/Thematic Mapper (L5/TM), L8/OLI and S2/MSI data over the period 2013–2019. An updated method was developed to improve the retrieval of glacier albedo by correcting glacier reflectance for anisotropy, and a new dataset on glacier albedo was generated for the period 2001–2020. A detailed glacier energy and mass balance model was developed with the support of field experiments at the Parlung No. 4 Glacier and the 24 K Glacier, both in the Tibetan Plateau. Besides meteorological measurements, the field experiments included glaciological and hydrological measurements. The energy balance model was formulated in terms of enthalpy for easier treatment of water phase transitions. The model was applied to assess the spatial variability in glacier melt. In the Parlung No. 4 Glacier, the accumulated glacier melt was between 1.5 and 2.5 m w.e. in the accumulation zone and between 4.5 and 6.0 m w.e. in the ablation zone, reaching 6.5 m w.e. at the terminus. The seasonality in the glacier mass balance was observed by combining intensive field campaigns with continuous automatic observations. The linkage of the glacier and snowpack mass balance with water resources in a river basin was analyzed in the Chiese (Italy) and Heihe (China) basins by developing and applying integrated hydrological models using satellite retrievals in multiple ways. The model FEST-WEB was calibrated using retrievals of Land Surface Temperature (LST) to map soil hydrological properties. A watershed model was developed by coupling ecohydrological and socioeconomic systems. Integrated modeling is supported by an updated and parallelized data assimilation system. The latter exploits retrievals of brightness temperature (Advanced Microwave Scanning Radiometer, AMSR), LST (Moderate Resolution Imaging Spectroradiometer, MODIS), precipitation (Tropical Rainfall Measuring Mission (TRMM) and FengYun (FY)-2D) and in-situ measurements. In the case study on the Red River Basin, a new algorithm has been applied to disaggregate the SMOS (Soil Moisture and Ocean Salinity) soil moisture retrievals by making use of the correlation between evaporative fraction and soil moisture.
    Type of Medium: Online Resource
    ISSN: 2072-4292
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2513863-7
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  • 2
    In: Molecules, MDPI AG, Vol. 16, No. 2 ( 2011-02-15), p. 1642-1654
    Type of Medium: Online Resource
    ISSN: 1420-3049
    Language: English
    Publisher: MDPI AG
    Publication Date: 2011
    detail.hit.zdb_id: 2008644-1
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  • 3
    In: Genes, MDPI AG, Vol. 13, No. 9 ( 2022-08-29), p. 1558-
    Abstract: Pathogenic variants of zinc finger C4H2-type containing (ZC4H2) on the X chromosome cause a group of genetic diseases termed ZC4H2-associated rare disorders (ZARD), including Wieacker-Wolff Syndrome (WRWF) and Female-restricted Wieacker-Wolff Syndrome (WRWFFR). In the current study, a de novo c.352C 〉 T (p.Gln118*) mutation in ZC4H2 (NM_018684.4) was identified in a female neonate born with severe arthrogryposis multiplex congenita (AMC) and Pierre-Robin sequence (cleft palate and micrognathia). Plasmids containing the wild-type (WT), mutant-type (MT) ZC4H2, or GFP report gene (N) were transfected in 293T cell lines, respectively. RT-qPCR and western blot analysis showed that ZC4H2 protein could not be detected in the 293T cells transfected with MT ZC4H2. The RNA seq results revealed that the expression profile of the MT group was similar to that of the N group but differed significantly from the WT group, indicating that the c.352C 〉 T mutation resulted in the loss of function of ZC4H2. Differentially expressed genes (DEGs) enrichment analysis showed that c.352C 〉 T mutation inhibited the expression levels of a series of genes involved in the oxidative phosphorylation pathway. Subsequently, expression levels of ZC4H2 were knocked down in neural stem cells (NSCs) derived from induced pluripotent stem cells (iPSCs) by lentiviral-expressed small hairpin RNAs (shRNAs) against ZC4H2. The results also demonstrated that decreasing the expression of ZC4H2 significantly reduced the growth of NSCs by affecting the expression of genes related to the oxidative phosphorylation signaling pathway. Taken together, our results strongly suggest that ZC4H2 c.352C 〉 T (p.Gln118*) mutation resulted in the loss of protein function and caused WRWFFR.
    Type of Medium: Online Resource
    ISSN: 2073-4425
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2527218-4
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  • 4
    In: Cells, MDPI AG, Vol. 11, No. 20 ( 2022-10-14), p. 3229-
    Abstract: Oxidative damage is a critical cause of diabetic wounds. Exosomes from various stem cells could promote wound repair. Here, we investigated the potential mechanism by which exosomes from adipose-derived stem cells (ADSC-EXOs) promote diabetic wound healing through the modulation of oxidative stress. We found that ADSC-EXOs could promote proliferation, migration, and angiogenesis in keratinocytes, fibroblasts, and endothelial cells. Furthermore, ADSC-EXOs reduced the reactive oxygen species (ROS) levels in these cells and protected them against hypoxic and oxidative stress damage. Finally, the local injection of ADSC-EXOs at wound sites significantly increased collagen deposition and neovascularization while reducing ROS levels and cell death; thus, it led to accelerated diabetic wound closure. The mechanism underlying ADSC-EXO functions involved heat-shock protein 90 (HSP90) expressed on the cell surface; these functions could be inhibited by an anti-HSP90 antibody. Exosomal HSP90 could bind to the low-density lipoprotein receptor-related protein 1 (LRP1) receptor on the recipient cell membrane, leading to activation of the downstream AKT signaling pathway. Knockdown of LRP1 and inhibition of the AKT signaling pathway by LY294002 in fibroblasts was sufficient to impair the beneficial effect of ADSC-EXOs. In summary, ADSC-EXOs significantly accelerated diabetic wound closure through an exosomal HSP90/LRP1/AKT signaling pathway.
    Type of Medium: Online Resource
    ISSN: 2073-4409
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2661518-6
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  • 5
    In: Microorganisms, MDPI AG, Vol. 9, No. 7 ( 2021-07-19), p. 1536-
    Abstract: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used in the field of clinical microbiology since 2010. Compared with the traditional technique of biochemical identification, MALDI-TOF MS has many advantages, including convenience, speed, accuracy, and low cost. The accuracy and speed of identification using MALDI-TOF MS have been increasing with the development of sample preparation, database enrichment, and algorithm optimization. MALDI-TOF MS has shown promising results in identifying cultured colonies and rapidly detecting samples. MALDI-TOF MS has critical research applications for the rapid detection of highly virulent and drug-resistant pathogens. Here we present a scientific review that evaluates the performance of MALDI-TOF MS in identifying clinical pathogenic microorganisms. MALDI-TOF MS is a promising tool in identifying clinical microorganisms, although some aspects still require improvement.
    Type of Medium: Online Resource
    ISSN: 2076-2607
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2720891-6
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  • 6
    In: Sustainability, MDPI AG, Vol. 15, No. 4 ( 2023-02-10), p. 3247-
    Abstract: Due to the global warming and dynamic changes in pathogenic virulence, leaf rust caused by Puccinia triticina has greatly expanded its epidermic region and become a severe threat to global wheat production. Genetic bases of wheat resistance to leaf rust mainly rely on the leaf rust resistance (Lr) gene or quantitative trait locus (QLr). Although these genetic loci have been insensitively studied during the last two decades, an updated overview of Lr/QLr in a genome-wide level is urgently needed. This review summarized recent progresses of genetic studies of wheat resistance to leaf rust. Wheat germplasms with great potentials for genetic improvement in resistance to leaf rust were highlighted. Key information about the genetic loci carrying Lr/QLr was summarized. A genome-wide chromosome distribution map for all of the Lr/QLr was generated based on the released wheat reference genome. In conclusion, this review has provided valuable sources for both wheat breeders and researchers to understand the genetics of resistance to leaf rust in wheat.
    Type of Medium: Online Resource
    ISSN: 2071-1050
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2518383-7
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  • 7
    Online Resource
    Online Resource
    MDPI AG ; 2018
    In:  International Journal of Molecular Sciences Vol. 19, No. 12 ( 2018-11-22), p. 3706-
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 19, No. 12 ( 2018-11-22), p. 3706-
    Abstract: Innate immunity is an essential line of defense against pathogen invasion which is gained at birth, and the mechanism involved is mainly to identify pathogen-associated molecular patterns through pattern recognition receptors. STING (stimulator of interferon genes) is a signal junction molecule that hosts the perception of viral nucleic acids and produces type I interferon response, which plays a crucial role in innate immunity. However, relatively few studies have investigated the molecular characterization, tissue distribution, and potential function of STING in chickens. In this study, we cloned the full-length cDNA of chicken STING that is composed of 1341 bp. Sequence analyses revealed that STING contains a 1140-bp open-reading frame that probably encodes a 379-amino acid protein. Multiple sequence alignments showed that the similarity of the chicken STING gene to other birds is higher than that of mammals. Real-time polymerase chain reaction (PCR) assays revealed that STING is highly expressed in the spleen, thymus and bursa of fabricious in chickens. Furthermore, we observed that STING expression was significantly upregulated both in vitro and in vivo following infection with Newcastle disease virus (NDV). STING expression was also significantly upregulated in chicken embryo fibroblasts upon stimulation with poly(I:C) or poly(dA:dT). Taken together, these findings suggest that STING plays an important role in antiviral signaling pathways in chickens.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 8
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 19, No. 3 ( 2018-02-27), p. 675-
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 9
    In: Photonics, MDPI AG, Vol. 9, No. 1 ( 2022-01-17), p. 49-
    Abstract: Coherent beam combining (CBC) with closely arranged centrosymmetric arrays is a promising way to obtain a high-brightness laser. An essential task in CBC is to actively control the piston phases of the input beams, maintaining the correct phasing to maximize the combination efficiency. By applying the neural network, the nonlinear mapping relationship between the far-field image and the piston phase could be established, so that the piston phase can be corrected quickly with one step, which caused widespread concern. However, there exists a piston-type phase ambiguity problem in the CBC system with centrosymmetric arrays, which means that multiple different piston phases may generate the same far-field image. This will prevent the far-field image from correctly reflecting the phase information, which will result in a performance degradation of the image-based intelligent algorithms. In this paper, we make a theoretical analysis of phase ambiguity. A method to solve phase ambiguity is proposed, which requires no additional optical devices. We designed simulations to verify our conclusions and methods. We believe that our work solves the phase ambiguity problem in theory and is conducive to improving the performance of image-based algorithms.
    Type of Medium: Online Resource
    ISSN: 2304-6732
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2770002-1
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  • 10
    In: Fermentation, MDPI AG, Vol. 10, No. 5 ( 2024-04-25), p. 229-
    Abstract: To explore the key sites affecting the intracellular assembly of key components of cellulosomes and obtain DocA mutants independent of Ca2+, Swiss-model, GROMACS, PyMOL, and other molecular dynamics simulation software were used for modeling and static and dynamic combination analysis. Site-specific mutation technology was used to mutate DocA, and Biacore was used to test the dependence of Ca2+ on the binding ability of protein DocA mutants and protein Coh, and to analyze the interaction and binding effect of mutant proteins in vitro. Forward intracellular mutant screening was performed based on semi-rational design and high throughput screening techniques. The orientation of mutations suitable for intracellular assembly was determined, and three directional mutant proteins, DocA-S1, DocA-S2, and DocA-S3, were obtained. Ca2+ independent DocA mutants were obtained gradually and their potential interaction mechanisms were analyzed. In the present study, intracellular self-assembly of key components of cellulosomes independent of Ca2+ was achieved, and DocA-S3 was applied to the assembly of key enzymes of L-lysine biosynthesis, in which DapA and DapB intracellular assembly increased L-lysine accumulation by 29.8% when compared with the control strains, providing a new strategy for improving the intracellular self-assembly of cellulosomes and amino acid fermentation efficiency.
    Type of Medium: Online Resource
    ISSN: 2311-5637
    Language: English
    Publisher: MDPI AG
    Publication Date: 2024
    detail.hit.zdb_id: 2813985-9
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