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  • 1
    In: Diversity, MDPI AG, Vol. 15, No. 8 ( 2023-07-28), p. 894-
    Abstract: Dipterocarpus turbinatus C.F.Gaertn. is a valuable timber tree indigenous to the lowland tropical forests of southern and central Vietnam. It is in danger of extinction due to fragmented habitats and overexploitation. Therefore, assessing the genetic diversity and demographic history of this species is essential to providing a platform for conservation activities. Herein, we analyzed 281 D. turbinatus trees from 10 populations representing their natural distribution range in Vietnam using nine polymorphic microsatellites to provide valuable information for conservation activities. We detected genetic diversity within these populations (0.285 and 0.328 for observed and expected heterozygosity, respectively), as well as population genetic differentiation (a Wei and Cockerham value of 0.202 and a Hedrick value of 0.32). Clustering analysis based on different approaches revealed three genetic clusters which were significantly correlated to gene flow across the geographic distribution range of D. turbinatus in tropical evergreen forests. Approximate Bayesian computation suggested that the D. turbinatus populations had an evolutionary history consistent with divergence during the last glacial maximum. Based on our results, we recommend the in situ conservation of several populations with high genetic diversity, high allelic richness, or private alleles, and the gathering of the seeds of the remaining populations for ex situ conservation.
    Type of Medium: Online Resource
    ISSN: 1424-2818
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2518137-3
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  • 2
    In: Molecules, MDPI AG, Vol. 25, No. 17 ( 2020-08-22), p. 3825-
    Abstract: k-Oligocarrageenan (OC) is an effective biostimulator and a protector against disease infections for plants. However, the effect of OC molecular weight (MW) on plant growth is not fully understood. In this work, OCs with three different MWs (42, 17 and 4 kDa) was prepared by varying the degradation reaction time using ascorbic acid as a reagent. The product structure was confirmed by Fourier-transform infrared spectroscopy (FTIR) data. The growth promotion for maize (Zea mays L.) plants was investigated by foliar spray application of the prepared OCs. Field trials were carried out in two years, 2018 and 2019. The results showed that among treatments, OC with 4 kDa exhibited the best performance in both crop growth and grain yield parameters which indicated increases compared to the control in plant height (6.9–19.9%), length of ears (12.2%), diameter of ears (9.1%), fresh grain weight (17.8%), dry grain weight (20.0%) and grain yield (21.3%). Moreover, low MW OC augmented NP uptake in the plant growth while no effect on K uptake was observed. Therefore, OC with low MWs is potentially promising to apply as a promoter to enhance yield of crops.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2008644-1
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  • 3
    In: Agriculture, MDPI AG, Vol. 11, No. 5 ( 2021-04-27), p. 397-
    Abstract: Rice breeding was conducted for a long time during historical times and is an important job in Vietnam because rice is the major food for domestic consumption and export. In this review, we have provided a comprehensive insight into the importance of promising rice germplasm resources, breeding achievements, and breeding approaches as well as discussed challenges and perspectives of rice breeding in this country. With rice germplasm and wild rice relative resources with rich and various genetic diversity, their useful genes and traits have been exploited and integrated into commercial varieties as the final outputs of rice breeding programs. New achievements of the modern genetics era have been approached and effectively contributed to breeding activities in this country. Genome sequences, molecular breeding, and mutation are powerful tools and playing vital roles in developing new varieties with characteristics of interest that should be followed by the current market demands. In the last decades, there has been a plethora of newly generated varieties by Vietnamese scientists and rice breeders and approved by the state authorities. However, very few domestic mega varieties have prevailed over the imported varieties. Therefore, rice breeding in this country is faced with big challenges, including limitations of backgrounds, budgets, and even talents in basic research to compete with other rice-producing countries. The target goals and long-term approaches for rice breeding should be paid explicitly in priority to ensure national food security and the advantage and development of rice breeding in this country.
    Type of Medium: Online Resource
    ISSN: 2077-0472
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2651678-0
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  • 4
    In: Medicina, MDPI AG, Vol. 55, No. 4 ( 2019-04-12), p. 102-
    Abstract: Background and Objectives: Congenital nephrotic syndrome (CNS), a genetic disease caused by mutations in genes on autosomes, usually occurs in the first three months after birth. A number of genetic mutations in genes, which encode for the components of the glomerular filtration barrier have been identified. We investigated mutations in NPHS1, NPHS2, PLCE1 (NPHS3), and WT1 genes that relate to the disease in Vietnamese patients. Materials and Methods: We performed genetic analysis of two unrelated patients, who were diagnosed with CNS in the Vietnam National Children’s Hospital with different disease status. The entire coding region and adjacent splice sites of these genes were amplified and sequenced using the Sanger method. The sequencing data were analyzed and compared with the NPHS1, NPHS2, PLCE1, and WT1 gene sequences published in Ensembl (ENSG00000161270, ENSG00000116218, ENSG00000138193, and ENSG00000184937, respectively) using BioEdit software to detect mutations. Results: We detected a new variant p.Ser607Arg and two other (p.Glu117Lys and p.Ser1105Ser) in the NPHS1 gene, as well as two variants (p.Arg548Leu, p.Pro1575Arg) in the PLCE1 gene. No mutations were detected in the NPHS2 and WT1 genes. Patient 1, who presented a heterozygous genotype of p.Ser1105Ser and p.Arg548Leu had a mild disease status but patient 2, who presented a homozygous genotype of these alleles, had a severe phenotype. Conclusions: These results suggest that variants p.Ser1105Ser (in NPHS1 gene) and p.Arg548Leu (in PLCE1 gene) in the homozygous form might play a role in the development of the disease in patients.
    Type of Medium: Online Resource
    ISSN: 1648-9144
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2088820-X
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  • 5
    In: International Journal of Environmental Research and Public Health, MDPI AG, Vol. 15, No. 5 ( 2018-05-14), p. 981-
    Type of Medium: Online Resource
    ISSN: 1660-4601
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2175195-X
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  • 6
    In: Coatings, MDPI AG, Vol. 12, No. 8 ( 2022-07-24), p. 1046-
    Abstract: Spherical zinc is well known as an effective pigment for the corrosion protection of carbon steel. However, a high proportion of spherical Zn in a coating leads to difficulties in fabricating the coating solution and increased cost. In this work, the influence of flake ZnAl alloy in silicate coatings on the corrosion protection properties of steel substrates was investigated. The electrochemical behaviour of coatings containing different flake ZnAl alloy content immersed in NaCl solution (3.5 wt%) was evaluated using an electrochemical impedance spectroscopy (EIS) method. A salt spray test was performed to evaluate corrosion on the coating surface. Pull-off tests of the coatings before and after the salt spray process were performed, and the surface morphology was analysed to determine the degradation of corrosion resistance. The results show that silicate coating containing flake ZnAl alloy (25 wt%) possesses the highest total resistance (1417 Ω) and the longest time to the appearance of white rust (720 h). The surface morphology of the coating containing 25 wt% flake ZnAl alloy was found to include corrosion products with the most compacted surface, which effectively prevents the penetration of electrolytes to the interface between the coating and the steel.
    Type of Medium: Online Resource
    ISSN: 2079-6412
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2662314-6
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  • 7
    In: Current Issues in Molecular Biology, MDPI AG, Vol. 43, No. 3 ( 2021-12-10), p. 2210-2219
    Abstract: Astronauts are always faced with serious health problems during prolonged spaceflights. Previous studies have shown that weightlessness significantly affects the physiological function of female astronauts, including a change in reproductive hormones and ovarian cells, such as granulosa and theca cells. However, the effects of microgravity on these cells have not been well characterized, especially in granulosa cells. This study aimed to investigate the effects of simulated microgravity (SMG) on the proliferation and morphology of porcine granulosa cells (pGCs). pGC proliferation from the SMG group was inhibited, demonstrated by the reduced O.D. value and cell density in the WST-1 assay and cell number counting. SMG-induced pGCs exhibited an increased ratio of cells in the G0/G1 phase and a decreased ratio of cells in the S and G2/M phase. Western blot analysis indicated a down-regulation of cyclin D1, cyclin-dependent kinase 4 (cdk4), and cyclin-dependent kinase 6 (cdk6), leading to the prevention of the G1-S transition and inducing the arrest phase. pGCs under the SMG condition showed an increase in nuclear area. This caused a reduction in nuclear shape value in pGCs under the SMG condition. SMG-induced pGCs exhibited different morphologies, including fibroblast-like shape, rhomboid shape, and pebble-like shape. These results revealed that SMG inhibited proliferation and induced morphological changes in pGCs.
    Type of Medium: Online Resource
    ISSN: 1467-3045
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2090836-2
    SSG: 12
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  • 8
    In: Molecules, MDPI AG, Vol. 28, No. 17 ( 2023-09-04), p. 6433-
    Abstract: Curcumin (CUR) is a natural compound extracted from turmeric (Curcuma longa L.) used to cure acne, wound healing, etc. Its disadvantages, such as poor solubility and permeability, limit its efficacy. Nanoemulsion (NE)-based drug delivery systems have gained popularity due to their advantages. This study aimed to optimize a CUR-NE-based gel and evaluate its physicochemical and biological properties. A NE was prepared using the catastrophic phase inversion method and optimized using the Design Expert 12.0 software. The CUR-NE gel was characterized in terms of visual appearance, pH, drug release, antibacterial and wound healing effects. The optimal formulation contained CUR, Capryol 90 (oil), Labrasol:Cremophor RH40 (1:1) (surfactants), propylene glycol (co-surfactant), and water. The NE had a droplet size of 22.87 nm and a polydispersity index of 0.348. The obtained CUR-NE gel had a soft, smooth texture and a pH of 5.34 ± 0.05. The in vitro release of CUR from the NE-based gel was higher than that from a commercial gel with nanosized CUR (21.68 ± 1.25 µg/cm2, 13.62 ± 1.63 µg/cm2 after 10 h, respectively). The CUR-NE gel accelerated in vitro antibacterial and in vivo wound healing activities as compared to other CUR-loaded gels. The CUR-NE gel has potential for transdermal applications.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2008644-1
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  • 9
    In: Geriatrics, MDPI AG, Vol. 6, No. 4 ( 2021-12-12), p. 116-
    Abstract: The proportion of geriatric depression recorded in Vietnam was 66.9%. Depression in older people is a risk factor for problems related to dementia, poor quality of life, and suicide. To have a good Vietnamese questionnaire for assessing geriatric depression, we conducted the study to translate and cross-culturally adapt the Geriatric Depression Scale—long-form with 30 items (GDS-30). The study has two steps. Step 1 is a translation of the GDS-30 scale. We followed the guideline by Beaton et al., (2000 & 2007). Firstly, two translators (informed and uninformed) translated the questionnaires. Secondly, the translations were synthesized. Thirdly, back translation was performed by two translators fluent in both Vietnamese and English but completely unknown of the original version of the scale and did not have medical expertise. Finally, seven experts reached a consensus on the pre-final Vietnamese version (GDS-30). Step 2 is a field test of the questionnaires on people 60 years or older. Then, we determined the internal consistency and test-retest reliability of the questionnaire in 55 Vietnamese inpatients in a geriatric department. Construct validity was determined by examining the relationship between depressive scores and patient characteristics. The Vietnamese version of GDS-30 was built with the agreement of all experts on the semantic, idiomatic, experiential, and conceptual equivalences between the original and pre-final Vietnamese versions of the GDS-30. The Cronbach’s alpha coefficient value was 0.928, indicating the items’ adequate internal consistency. Spearman’s correlation coefficient value of total scores between the first and second interviews showed medium correlation (0.479, p 〈 0.001), and the stability is acceptable. The GDS-30 scale reached the construct validity because the proportion of geriatric depression according to GDS-30 was significantly different between characteristics groups, such as gender, employment, level of education, economic status, and sleep disturbance. The Vietnamese version of the GDS-30 scale had high consistency, satisfactory reliability, and understanding and can be used as a screening tool for depression in elderly patients in primary healthcare centers. This is the first depression rating scale for the elderly in Vietnam to be translated and validated. Non-psychiatric health professionals or patients can quickly self-assess and screen for the illness.
    Type of Medium: Online Resource
    ISSN: 2308-3417
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2934571-6
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  • 10
    In: Healthcare, MDPI AG, Vol. 11, No. 11 ( 2023-06-05), p. 1658-
    Abstract: Background: Helicobacter pylori (H. pylori) remains a major cause of gastroduodenal diseases. We aimed to evaluate the burden of this infection, particularly peptic ulcer disease in Vietnamese children. Methods: We enrolled consecutive children referred for esophagogastroduodenoscopy at two tertiary children’s hospitals in Ho Chi Minh City, from October 2019 to May 2021. Children treated with proton pump inhibitors during the last two weeks or antibiotics for four weeks, and those having a previous or interventional endoscopy were excluded. H. pylori infection was diagnosed with either a positive culture or positive histopathology combined with a rapid urease test, or with a polymerase chain reaction of the urease gene. The study was approved by the Ethics Committee and written informed consent/assent was obtained. Results: Among 336 enrolled children aged 4–16 (mean: 9.1 ± 2.4 years; 55.4% girls), H. pylori infection was positive in 80%. Peptic ulcers were detected in 65 (19%), increasing with age, and 25% with anemia. cagA+ strains were detected at a higher rate in children with ulcers. Conclusions: Prevalence of H. pylori and peptic ulcers is high among symptomatic Vietnamese children. It is crucial to have a program for early detection of H. pylori to reduce ulcer risk and gastric cancer later.
    Type of Medium: Online Resource
    ISSN: 2227-9032
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2721009-1
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