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  • MDPI AG  (10)
  • 1
    In: Sustainability, MDPI AG, Vol. 14, No. 24 ( 2022-12-07), p. 16381-
    Abstract: Soil moisture is one of the most critical elements of the Earth system and is essential for the study of the terrestrial water cycle, ecological processes, climate change, and disaster warnings. In this study, the training sample was selected to divide the dataset according to months from 2000 to 2018 after the advantages of three training samples were compared using a backpropagation (BP) neural network model. Furthermore, the monthly surface soil moisture in China in 2019 and 2020 was simulated based on various meteorological elements. The results demonstrate that evapotranspiration has the greatest influence on soil moisture among the various meteorological factors, followed by precipitation on a national scale throughout the year. Additionally, the accuracy of the training and simulation results with BP neural networks in the national winter months is slightly worse. In the future, the training samples of the BP neural network can be optimized following the differences in the dominant influence of various meteorological factors on soil moisture in different areas at different times to improve the simulation prediction accuracy.
    Type of Medium: Online Resource
    ISSN: 2071-1050
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2518383-7
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  • 2
    In: Metabolites, MDPI AG, Vol. 13, No. 7 ( 2023-07-11), p. 836-
    Abstract: Myostatin (MSTN) is a negative regulator of skeletal muscle genesis during development. MSTN mutation leads to increased lean meat production and reduced fat deposition in livestock. However, the mechanism by which MSTN promotes myogenesis by regulating metabolism is not clear. In this study, we compared the metabolomics of the livers of wild-type (WT) and MSTN mutation cattle (MT), and found changes in the content and proportion of fatty acids and bile acids in MT cattle. The differential metabolites were enriched in sterol synthesis and primary bile acid synthesis. We further analyzed the expression of genes involved in the regulation of lipid and bile acid metabolism, and found that the loss of MSTN may alter lipid synthesis and bile acid metabolism. This study provides new basic data for MSTN mutations in beef cattle breeding.
    Type of Medium: Online Resource
    ISSN: 2218-1989
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2662251-8
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  • 3
    In: Animals, MDPI AG, Vol. 12, No. 2 ( 2022-01-15), p. 205-
    Abstract: Myostatin (MSTN) is a major negative regulator of skeletal muscle mass and causes a variety of metabolic changes. However, the effect of MSTN knockout on bile acid metabolism has rarely been reported. In this study, the physiological and biochemical alterations of serum in MSTN+/− and wild type (WT) cattle were investigated. There were no significant changes in liver and kidney biochemical indexes. However, compared with the WT cattle, lactate dehydrogenase, total bile acid (TBA), cholesterol, and high-density lipoprotein (HDL) in the MSTN+/− cattle were significantly increased, and glucose, low-density lipoprotein (LDL), and triglycerides (TG) were significantly decreased, indicating that MSTN knockout affected glucose and lipid metabolism and total bile acids content. Targeted metabolomic analysis of the bile acids and their derivatives was performed on serum samples and found that bile acids were significantly increased in the MSTN+/− cattle compared with the WT cattle. As the only bile acid synthesis organ in the body, we performed metabolomic analysis on the liver to study the effect of MSTN knockout on hepatic metabolism. Metabolic pathway enrichment analysis of differential metabolites showed significant enrichment of the primary bile acid biosynthesis and bile secretion pathway in the MSTN+/− cattle. Targeted metabolomics data further showed that MSTN knockout significantly increased bile acid content in the liver, which may have resulted from enhanced bile acid synthesis due to the expression of bile acid synthesis genes, cholesterol 7 alpha-hydroxylase (CYP7A1) and sterol 27-hydroxylase (CYP27A1), and upregulation in the liver of the MSTN+/− cattle. These results indicate that MSTN knockout does not adversely affect bovine fitness but regulates bile acid metabolism via enhanced bile acid synthesis. This further suggests a role of MSTN in regulating metabolism.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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  • 4
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 23, No. 17 ( 2022-09-04), p. 10145-
    Abstract: Inter-species somatic cell nuclear transfer (iSCNT) is significant in the study of biological problems such as embryonic genome activation and the mitochondrial function of embryos. Here, we used iSCNT as a model to determine whether abnormal embryo genome activation was caused by mitochondrial dysfunction. First, we found the ovine-bovine iSCNT embryos were developmentally blocked at the 8-cell stage. The reactive oxygen species level, mitochondrial membrane potential, and ATP level in ovine-bovine cloned embryos were significantly different from both bovine-bovine and IVF 8-cell stage embryos. RNA sequencing and q-PCR analysis revealed that mitochondrial transport, mitochondrial translational initiation, mitochondrial large ribosomal subunit, and mitochondrial outer membrane genes were abnormally expressed in the ovine-bovine embryos, and the mitochondrial outer membrane and mitochondrial ribosome large subunit genes, mitochondrial fusion gene 1, and ATPase Na+/K+ transporting subunit beta 3 gene were expressed at lower levels in the ovine-bovine cloned embryos. Furthermore, we found that overexpression and knockdown of Mfn1 significantly affected mitochondrial fusion and subsequent biological functions such as production of ATP, mitochondrial membrane potential, reactive oxygen species and gene expressions in cloned embryos. These findings enhance our understanding of the mechanism by which the Mfn1 gene regulates embryonic development and embryonic genome activation events.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 5
    In: Animals, MDPI AG, Vol. 12, No. 8 ( 2022-04-18), p. 1047-
    Abstract: In precision dairy farming, computer vision-based approaches have been widely employed to monitor the cattle conditions (e.g., the physical, physiology, health and welfare). To this end, the accurate and effective identification of individual cow is a prerequisite. In this paper, a deep learning re-identification network model, Global and Part Network (GPN), is proposed to identify individual cow face. The GPN model, with ResNet50 as backbone network to generate a pooling of feature maps, builds three branch modules (Middle branch, Global branch and Part branch) to learn more discriminative and robust feature representation from the maps. Specifically, the Middle branch and the Global branch separately extract the global features of middle dimension and high dimension from the maps, and the Part branch extracts the local features in the unified block, all of which are integrated to act as the feature representation for cow face re-identification. By performing such strategies, the GPN model not only extracts the discriminative global and local features, but also learns the subtle differences among different cow faces. To further improve the performance of the proposed framework, a Global and Part Network with Spatial Transform (GPN-ST) model is also developed to incorporate an attention mechanism module in the Part branch. Additionally, to test the efficiency of the proposed approach, a large-scale cow face dataset is constructed, which contains 130,000 images with 3000 cows under different conditions (e.g., occlusion, change of viewpoints and illumination, blur, and background clutters). The results of various contrast experiments show that the GPN outperforms the representative re-identification methods, and the improved GPN-ST model has a higher accuracy rate (up by 2.8% and 2.2% respectively) in Rank-1 and mAP, compared with the GPN model. In conclusion, using the Global and Part feature deep network with attention mechanism can effectively ameliorate the efficiency of cow face re-identification.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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  • 6
    In: Sustainability, MDPI AG, Vol. 14, No. 3 ( 2022-01-25), p. 1358-
    Abstract: The fitness industry is rapidly developing due to the demand for fitness activities, and a large number of commercial fitness spaces have emerged in Changchun city. The distribution of commercial fitness spaces in the city is not chaotic; different types of fitness spaces should have different spaces to choose from. The purpose of this article is to summarize the spatial distribution characteristics and laws of urban commercial fitness spaces, to help better develop commercial fitness spaces. Using Changchun (a central city in northeastern China) as an example, the article divides commercial fitness spaces into five categories. Then, GIS tools are used to analyze the distribution patterns, level distributions, and agglomeration characteristics of commercial fitness spaces. The city’s commercial fitness space distribution patterns are subjected to further study, along with the influencing factors and forming mechanisms of the pattern. Moreover, based on the research results, this study provides targeted suggestions for the development of fitness spaces. The study found that the commercial fitness space in Changchun city has formed a multi-core spatial pattern. Various types of fitness spaces show significant spatial differentiation in many aspects, such as “center-periphery” characteristics, the spatial distribution form, and the specialized characteristics of each block unit. Fitness needs, national policies, transportation accessibility, spatial agglomeration, land rent, urban population distribution, etc., are the main factors affecting the spatial distributions of fitness spaces.
    Type of Medium: Online Resource
    ISSN: 2071-1050
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2518383-7
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  • 7
    In: Plants, MDPI AG, Vol. 10, No. 3 ( 2021-03-17), p. 570-
    Abstract: The FHY3/FAR1 transcription factor family, derived from transposases, plays important roles in light signal transduction, and in the growth and development of plants. However, the homologous genes in tea plants have not been studied. In this study, 25 CsFHY3/FAR1 genes were identified in the tea plant genome through a genome-wide study, and were classified into five subgroups based on their phylogenic relationships. Their potential regulatory roles in light signal transduction and photomorphogenesis, plant growth and development, and hormone responses were verified by the existence of the corresponding cis-acting elements. The transcriptome data showed that these genes could respond to salt stress and shading treatment. An expression analysis revealed that, in different tissues, especially in leaves, CsFHY3/FAR1s were strongly expressed, and most of these genes were positively expressed under salt stress (NaCl), and negatively expressed under low temperature (4 °C) stress. In addition, a potential interaction network demonstrated that PHYA, PHYC, PHYE, LHY, FHL, HY5, and other FRSs were directly or indirectly associated with CsFHY3/FAR1 members. These results will provide the foundation for functional studies of the CsFHY3/FAR1 family, and will contribute to the breeding of tea varieties with high light efficiency and strong stress resistance.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2704341-1
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  • 8
    Online Resource
    Online Resource
    MDPI AG ; 2022
    In:  Polymers Vol. 14, No. 4 ( 2022-02-11), p. 683-
    In: Polymers, MDPI AG, Vol. 14, No. 4 ( 2022-02-11), p. 683-
    Abstract: Ultrasonic plastic welding (UPW) is a promising method for joining carbon fiber reinforced thermoplastic (CFRTP). The interface temperature determines weld quality to a large extent. This paper numerically analyzes the contact behavior and temperature characterization during welding using harmonic balance method (HBM). The simulation and experimental results show that amplitude and welding time are important factors determining the interface temperature. Increasing amplitude and welding time can significantly increase the interface temperature. Plunging speed and trigger force have little effect on the interface temperature. For nonlinear contact and heat generation, the results show that there is a certain separation between workpieces and the heat source is mainly friction heat generation in the early stage of welding. With the progress of welding, there is no separation between the workpieces and viscoelastic heat generation begins to dominate.
    Type of Medium: Online Resource
    ISSN: 2073-4360
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2527146-5
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  • 9
    In: Life, MDPI AG, Vol. 12, No. 5 ( 2022-04-23), p. 627-
    Abstract: Chinese Yellow Cattle, an ancient and domesticated breed for draft service, provide unique animal genetic resources with excellent genetic features, including crude feed tolerance, good stress resistance, strong adaptability, and tender meat quality; however, their production performance and meat yield are significantly inferior. Herein, the myostatin gene (MSTN), a negative regulator of skeletal muscle development, was knocked out by CRISPR/Cas9 technology. Eight MSTN gene-edited bull calves (MT) were born, and six of them are well-developed. Compared with the control cattle (WT), the growth trait indexes of MT cattle were generally increased, and the hindquarters especially were significantly improved. The biochemical indexes and the semen characteristics demonstrated that MT bulls were healthy and fertile. Consistent with our conjecture, the wobble and beating of MT bull spermatozoa were significantly higher than that of WT. Nine sperm motility-related proteins and nineteen mitochondrial-related proteins were identified by up-regulation in MT bull spermatozoa using FLQ proteomic technique and act to govern sperm flagellum assembly, organization, and beating and provide sufficient energy for sperm motility. The current study confirmed that the MSTN gene-edited Chinese Yellow cattle have improved growth traits and normal fertility, which can be used for beef cattle production and breeding.
    Type of Medium: Online Resource
    ISSN: 2075-1729
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2662250-6
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  • 10
    In: Animals, MDPI AG, Vol. 13, No. 12 ( 2023-06-20), p. 2036-
    Abstract: Today, large-scale Penaeus monodon farms no longer incubate eggs but instead purchase larvae from large-scale hatcheries for rearing. The accurate counting of tens of thousands of larvae in these transactions is a challenging task due to the small size of the larvae and the highly congested scenes. To address this issue, we present the Penaeus Larvae Counting Strategy (PLCS), a simple and efficient method for counting Penaeus monodon larvae that only requires a smartphone to capture images without the need for any additional equipment. Our approach treats two different types of keypoints as equip keypoints based on keypoint regression to determine the number of shrimp larvae in the image. We constructed a high-resolution image dataset named Penaeus_1k using images captured by five smartphones. This dataset contains 1420 images of Penaeus monodon larvae and includes general annotations for three keypoints, making it suitable for density map counting, keypoint regression, and other methods. The effectiveness of the proposed method was evaluated on a real Penaeus monodon larvae dataset. The average accuracy of 720 images with seven different density groups in the test dataset was 93.79%, outperforming the classical density map algorithm and demonstrating the efficacy of the PLCS.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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