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  • 1
    In: Molecules, MDPI AG, Vol. 23, No. 1 ( 2017-12-26), p. 41-
    Type of Medium: Online Resource
    ISSN: 1420-3049
    Language: English
    Publisher: MDPI AG
    Publication Date: 2017
    detail.hit.zdb_id: 2008644-1
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  • 2
    In: Water, MDPI AG, Vol. 15, No. 13 ( 2023-06-21), p. 2309-
    Abstract: The hydromechanical coupling behavior of rocks is widely present in the fields of rock mechanics and engineering studies. Analyzing and summarizing the relevant literature, the current status of experimental and coupling theory research on hydromechanical coupling is systematically described, the commonly used numerical simulation methods and their applications are briefly introduced, and the hydromechanical coupling problems in mining engineering, water conservancy, and hydropower engineering, slope engineering, tunneling engineering, and other fields are analyzed. Regarding the current status of studies on the hydromechanical coupling behavior of rocks, the test research aspect needs to further enhance the test studies on the triaxial shear permeability of rock material, and adopt a combination of macroscopic, fine, and microscopic methods to study the hydraulic coupling problems of rock materials from different scales. To couple theory, the traditional concepts are broken through, and new coupling theories and mathematical models are used to explain and solve the relevant practical problems. Meanwhile, the application of interdisciplinary approaches to solving coupling problems in the future is emphasized. In terms of numerical simulation and engineering applications, new large data algorithms are developed to improve the efficiency of simulation calculations. In addition, consideration should be given to the numerical simulation of coupling effects, the coupled rheological effects, and the coupled dynamic properties of rock masses under high-ground stress and high water pressure.
    Type of Medium: Online Resource
    ISSN: 2073-4441
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2521238-2
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  • 3
    In: Viruses, MDPI AG, Vol. 12, No. 2 ( 2020-02-18), p. 225-
    Abstract: Influenza A viruses (IAV) have been a major public health threat worldwide, and options for antiviral therapy become increasingly limited with the emergence of drug-resisting virus strains. New and effective anti-IAV drugs, especially for highly pathogenic influenza, with different modes of action, are urgently needed. The influenza virus glycoprotein hemagglutinin (HA) plays critical roles in the early stage of virus infection, including receptor binding and membrane fusion, making it a potential target for the development of anti-influenza drugs. In this study, we show that OA-10, a newly synthesized triterpene out of 11 oleanane-type derivatives, exhibited significant antiviral activity against four different subtypes of IAV (H1N1, H5N1, H9N2 and H3N2) replications in A549 cell cultures with EC50 ranging from 6.7 to 19.6 μM and a negligible cytotoxicity (CC50 〉 640 μM). It inhibited acid-induced hemolysis in a dose-dependent manner, with an IC50 of 26 µM, and had a weak inhibition on the adsorption of H5 HA to chicken erythrocytes at higher concentrations (≥40 µM). Surface plasmon resonance (SPR) analysis showed that OA-10 interacted with HA in a dose-dependent manner with the equilibrium dissociation constants (KD) of the interaction of 2.98 × 10−12 M. Computer-aided molecular docking analysis suggested that OA-10 might bind to the cavity in HA stem region which is known to undergo significant rearrangement during membrane fusion. Our results demonstrate that OA-10 inhibits H5N1 IAV replication mainly by blocking the conformational changes of HA2 subunit required for virus fusion with endosomal membrane. These findings suggest that OA-10 could serve as a lead for further development of novel virus entry inhibitors to prevent and treat IAV infections.
    Type of Medium: Online Resource
    ISSN: 1999-4915
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2516098-9
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