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  • 1
    Online Resource
    Online Resource
    BSA Hydrogel Beads Functionalized with a Specific Aptamer Library for Capturing Pseudomonas aeruginosa in Serum and Blood
    MDPI AG ; 2021
    In:  International Journal of Molecular Sciences Vol. 22, No. 20 ( 2021-10-15), p. 11118-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 22, No. 20 ( 2021-10-15), p. 11118-
    Abstract: Systemic blood stream infections are a major threat to human health and are dramatically increasing worldwide. Pseudomonas aeruginosa is a WHO-alerted multi-resistant pathogen of extreme importance as a cause of sepsis. Septicemia patients have significantly increased survival chances if sepsis is diagnosed in the early stages. Affinity materials can not only represent attractive tools for specific diagnostics of pathogens in the blood but can prospectively also serve as the technical foundation of therapeutic filtration devices. Based on the recently developed aptamers directed against P. aeruginosa, we here present aptamer-functionalized beads for specific binding of this pathogen in blood samples. These aptamer capture beads (ACBs) are manufactured by crosslinking bovine serum albumin (BSA) in an emulsion and subsequent functionalization with the amino-modified aptamers on the bead surface using the thiol- and amino-reactive bispecific crosslinker PEG4-SPDP. Specific and quantitative binding of P. aeruginosa as the dedicated target of the ACBs was demonstrated in serum and blood. These initial but promising results may open new routes for the development of ACBs as a platform technology for fast and reliable diagnosis of bloodstream infections and, in the long term, blood filtration techniques in the fight against sepsis.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms222011118
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Albumin Microspheres as “Trans-Ferry-Beads” for Easy Cell Passaging in Cell Culture Technology
    MDPI AG ; 2021
    In:  Gels Vol. 7, No. 4 ( 2021-10-21), p. 176-
    Details
    In: Gels, MDPI AG, Vol. 7, No. 4 ( 2021-10-21), p. 176-
    Abstract: Protein hydrogels represent ideal materials for advanced cell culture applications, including 3D-cultivation of even fastidious cells. Key properties of fully functional and, at the same time, economically successful cell culture materials are excellent biocompatibility and advanced fabrication processes allowing their easy production even on a large scale based on affordable compounds. Chemical crosslinking of bovine serum albumin (BSA) with N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) in a water-in-oil emulsion with isoparaffinic oil as the continuous phase and sorbitan monooleate as surfactant generates micro-meter-scale spherical particles. They allow a significant simplification of an indispensable and laborious step in traditional cell culture workflows. This cell passaging (or splitting) to fresh culture vessels/flasks conventionally requires harsh trypsinization, which can be omitted by using the “trans-ferry-beads” presented here. When added to different pre-cultivated adherent cell lines, the beads are efficiently boarded by cells as passengers and can be easily transferred afterward for the embarkment of novel flasks. After this procedure, cells are perfectly viable and show normal growth behavior. Thus, the trans-ferry-beads not only may become extremely affordable as a final product but also may generally replace trypsinization in conventional cell culture, thereby opening new routes for the establishment of optimized and resource-efficient workflows in biological and medical cell culture laboratories.
    Type of Medium: Online Resource
    ISSN: 2310-2861
    URL: Article
    DOI: 10.3390/gels7040176
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2813982-3
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  • 3
    Online Resource
    Online Resource
    Correction: Patrick Nadoll et al. REY and Trace Element Chemistry of Fluorite from Post-Variscan Hydrothermal Veins in Paleozoic Units of the North German Basin. Geosciences, 2018, 8, 283
    MDPI AG ; 2018
    In:  Geosciences Vol. 8, No. 11 ( 2018-11-05), p. 403-
    Details
    In: Geosciences, MDPI AG, Vol. 8, No. 11 ( 2018-11-05), p. 403-
    Abstract: The authors would like to correct the published article [...]
    Type of Medium: Online Resource
    ISSN: 2076-3263
    URL: Article
    DOI: 10.3390/geosciences8110403
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2655946-8
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  • 4
    Online Resource
    Online Resource
    Polyclonal Aptamer Libraries from a FluRoot-SELEX for the Specific Labeling of the Apical and Elongation/Differentiation Zones of Arabidopsis thaliana Roots
    MDPI AG ; 2022
    In:  International Journal of Molecular Sciences Vol. 23, No. 20 ( 2022-10-13), p. 12220-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 23, No. 20 ( 2022-10-13), p. 12220-
    Abstract: In more than 30 years of aptamer research, it has become widely accepted that aptamers are fascinating binding molecules for a vast variety of applications. However, the majority of targets have been proteins, although special variants of the so-called SELEX process for the molecular evolution of specific aptamers have also been developed, allowing for the targeting of small molecules as well as larger structures such as cells and even cellular networks of human (tumor) tissues. Although the provocative thesis is widely accepted in the field, that is, in principle, any level of complexity for SELEX targets is possible, the number of studies on whole organs or at least parts of them is limited. To pioneer this thesis, and based on our FluCell-SELEX process, here, we have developed polyclonal aptamer libraries against apices and the elongation/differentiation zones of plant roots as examples of organs. We show that dedicated libraries can specifically label the respective parts of the root, allowing us to distinguish them in fluorescence microscopy. We consider this achievement to be an initial but important evidence for the robustness of this SELEX variant. These libraries may be valuable tools for plant research and a promising starting point for the isolation of more specific individual aptamers directed against root-specific epitopes.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms232012220
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Administration of Bovine Milk Oligosaccharide to Weaning Gnotobiotic Mice Inoculated with a Simplified Infant Type Microbiota
    MDPI AG ; 2021
    In:  Microorganisms Vol. 9, No. 5 ( 2021-05-06), p. 1003-
    Details
    In: Microorganisms, MDPI AG, Vol. 9, No. 5 ( 2021-05-06), p. 1003-
    Abstract: Bovine milk oligosaccharides (BMO) share structural similarity to selected human milk oligosaccharides, which are natural prebiotics for infants. Thus, there is a potential in including BMOs as a prebiotic in infant formula. To examine the in vivo effect of BMO-supplementation on the infant gut microbiota, a BMO-rich diet (2% w/w) was fed to gnotobiotic mice (n = 11) inoculated with an infant type co-culture and compared with gnotobiotic mice receiving a control diet (n = 9). Nuclear magnetic resonance metabolomics in combination with high-throughput 16S rRNA gene amplicon sequencing was used to compare metabolic activity and microbiota composition in different compartments of the lower gastrointestinal tract. BMO components were detected in cecum and colon contents, revealing that BMO was available for the gut bacteria. The gut microbiota was dominated by Enterobacteriaceae and minor abundance of Lactobacilliaceae, while colonization of Bifidobacteriaceae did not succeed. Apart from a lower E. coli population in cecum content and lower formate (in colon) and succinate (in colon and cecum) concentrations, BMO supplementation did not result in significant changes in microbiota composition nor metabolic activity. The present study corroborates the importance of the presence of bifidobacteria for obtaining microbial-derived effects of milk oligosaccharides in the gastrointestinal tract.
    Type of Medium: Online Resource
    ISSN: 2076-2607
    URL: Article
    DOI: 10.3390/microorganisms9051003
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2720891-6
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  • 6
    Online Resource
    Online Resource
    Perspectives from Dentists, Dental Assistants, Students, and Patients on Dental Care Adapted to the COVID-19 Pandemic: A Cross-Sectional Survey
    MDPI AG ; 2021
    In:  International Journal of Environmental Research and Public Health Vol. 18, No. 8 ( 2021-04-09), p. 3940-
    Details
    In: International Journal of Environmental Research and Public Health, MDPI AG, Vol. 18, No. 8 ( 2021-04-09), p. 3940-
    Abstract: Dental care has been affected by SARS-CoV-2 (COVID-19) worldwide. In contrast to other dental clinics, the Justus-Liebig-University Giessen (Germany) decided not to limit dental treatment to emergencies alone, but to continue dental care for all patients, with increased safety measures. As such, health care professionals may be exposed to additional physical and mental stress. The aim of this study was to assess the perspectives of all persons involved in dental care (dentists, dental assistants, students, and patients) regarding the aspects of safety measures, anxiety about self-infection and infecting others, and other prospects in the period March to December 2020 using a questionnaire. Data collection was performed between 14 December 2020 and 23 January 2021. A total of 35 dentists (response rate of 79.5%), 23 dental assistants (65.7%), 84 students (80%), and 51 patients (21.8%) completed the survey. The patients did not notice any changes in the care received. Dentists and dental assistants reported a higher workload due to additional safety measures. The majority of dentists, students, and patients agreed that normal patient care was maintained. One-third of dental assistants would have preferred emergency treatment alone and expressed significantly higher anxiety about COVID-19 infection than all other groups (p 〈 0.05). In conclusion, all groups showed a predominantly positive perspective on dental care, and anxiety about self-infection and infecting others was especially low. However, additional measures are time-consuming and compound daily patient care. This concept, based on well-established infection control, might be a viable proposal for current and future pandemics.
    Type of Medium: Online Resource
    ISSN: 1660-4601
    URL: Article
    DOI: 10.3390/ijerph18083940
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2175195-X
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  • 7
    Online Resource
    Online Resource
    REY and Trace Element Chemistry of Fluorite from Post-Variscan Hydrothermal Veins in Paleozoic Units of the North German Basin
    MDPI AG ; 2018
    In:  Geosciences Vol. 8, No. 8 ( 2018-07-29), p. 283-
    Details
    In: Geosciences, MDPI AG, Vol. 8, No. 8 ( 2018-07-29), p. 283-
    Abstract: Hydrothermal fluorites from Paleozoic sedimentary rocks and volcanic units in the North German Basin (NGB) have been investigated to create a petrographic and geochemical inventory—with particular focus on strategic elements such as rare earth elements (REE)—and to uncover possible links between the post-Variscan hydrothermal mineralization in the NGB and bordering areas such as the Harz Mountains and Flechtingen Calvörde Block (FCB). Fluorites from ten localities underwent a detailed petrographic examination, including SEM-BSE/CL imagery, and were compositionally analysed using LA-ICP-MS. Overall, REY concentrations are comparatively low in fluorite from all investigated areas—the median sum of REY ranges from 0.3 to 176 ppm. EuropiumCN anomalies are slightly negative or absent, indicating that either the formation fluid experienced temperatures above 250 °C or that fluid-rock interactions and REE enrichment was likely controlled by the source rock (i.e., volcanic) composition and complexation processes. Fluorites from the Altmark-Brandenburg Basin (ABB) and the Lower Saxony Basin (LSB) display distinctly different REYCN signatures, suggesting that fluid compositions and genetic processes such as fluid-rock interaction differed significantly between the two areas. Complex growth zones and REYCN signatures in fluorite from the ABB and the FCB reflect geochemical variability due to adsorption processes and intrinsic crystallographic controls and imply that they are genetically related. Two petrographically and geochemically distinct generations are observed: Fluorite I—light SEM shades, relatively enriched in LREE; Fluorite II—darker SEM shades, comparatively depleted LREE, slightly higher HREE concentrations. These fluorite generations represent zoned (or cyclical) growth within a single progressive hydrothermal event and do not reflect a secondary remobilization process. We demonstrate that increasing Tb/La ratios and decreasing La/Ho ratios can be the result of continuous zoned growth during a single mineralizing event, with significant compositional variations on a micron-scale. This has implications for the interpretation of such trends and hence the inferred genetic evolution of fluorite that displays such geochemical patterns. The complex micro-scale intergrowth of these generations stresses the need for detailed petrographic investigations when geochemical data are collected and interpreted for mineral exploration.
    Type of Medium: Online Resource
    ISSN: 2076-3263
    URL: Article
    DOI: 10.3390/geosciences8080283
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2655946-8
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