GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • MDPI AG  (7)
  • 1
    Online Resource
    Online Resource
    Temporal Variation in Community Composition of Root Associated Endophytic Fungi and Carbon and Nitrogen Stable Isotope Abundance in Two Bletilla Species (Orchidaceae)
    MDPI AG ; 2020
    In:  Plants Vol. 10, No. 1 ( 2020-12-24), p. 18-
    Details
    In: Plants, MDPI AG, Vol. 10, No. 1 ( 2020-12-24), p. 18-
    Abstract: Mycorrhizae are an important energy source for orchids that may replace or supplement photosynthesis. Most mature orchids rely on mycorrhizae throughout their life cycles. However, little is known about temporal variation in root endophytic fungal diversity and their trophic functions throughout whole growth periods of the orchids. In this study, the community composition of root endophytic fungi and trophic relationships between root endophytic fungi and orchids were investigated in Bletilla striata and B. ochracea at different phenological stages using stable isotope natural abundance analysis combined with molecular identification analysis. We identified 467 OTUs assigned to root-associated fungal endophytes, which belonged to 25 orders in 10 phyla. Most of these OTUs were assigned to saprotroph (143 OTUs), pathotroph-saprotroph (63 OTUs) and pathotroph-saprotroph-symbiotroph (18 OTUs) using FunGuild database. Among these OTUs, about 54 OTUs could be considered as putative species of orchid mycorrhizal fungi (OMF). For both Bletilla species, significant temporal variation was observed in the diversity of root endophytic fungi. The florescence and emergence periods had higher fungal community richness of total species and endemic species than did other periods. Both Bletilla species were dominated by Agaricomycetes and Basidiomycota fungi throughout the whole year; however, their abundances varied between two Bletilla species and among phenological stages. Meanwhile, the ranges of 13C and 15N natural abundance were also highly dynamic across all growth stages of Bletilla species. Compared with the surrounding autotrophic plants, significant 13C enrichments (ε13C) were found across all phenological stages, while significant 15N enrichment in the florescence period and strong 15N depletion during the fruiting period were found for both Bletilla species. We can deduce that both Bletilla species obtained carbon from root endophytic fungi during the whole year. Additionally, the temporal varying tendency of root endophytic fungal diversity was consistent with 13C enrichments, which was also accord with the nutritional requirement of plant.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    URL: Article
    DOI: 10.3390/plants10010018
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2704341-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Relationship between Flower Color and Cellular Physicochemical Factors in Bletilla striata
    MDPI AG ; 2023
    In:  Horticulturae Vol. 9, No. 4 ( 2023-03-25), p. 426-
    Details
    In: Horticulturae, MDPI AG, Vol. 9, No. 4 ( 2023-03-25), p. 426-
    Abstract: Flower color not only determines the quality and commercial value of ornamental plants, but it also plays a vital role in ecological processes such as pollinator attraction. This study aimed to investigate the relationship between flower color and the cellular physicochemical factors of Bletilla striata. The color space values of 21 samples were initially determined, followed by a selection of five samples with significant color differences for testing cell shape, total flavonoid content (ranging from 1.86 to 5.42 mg/g), total anthocyanin content (ranging from 0.52 to 292.62 (A530 − 0.25 ∗ A657)/g), cell pH (varying between 5.03 and 5.74), and metal ion content (including Al3+, Ca2+, Fe3+, K+, Mg2+, Na+, P5+, Zn2+, Mo6+, Cu2+, and Mn2+). The flowers of Bletilla were predominantly purple, pink, and yellow; and distributed in quadrants I, II, and IV on the a* and b* rectangular coordinate. The a* value was identified as the primary color indicator for this species. Total anthocyanin content and Zn2+ showed a significant positive correlation with a*, while Al3+, Ca2+, Fe3+, Mg2+, Na+, and Mn2+ demonstrated negative correlations. Cell shape, flavonoid content, and pH had nonsignificant correlations with a*. In conclusion, the total anthocyanin content and metal ions play crucial roles in determining the flower color of B. striata, which can have implications for pollinator attraction. Future research should focus on understanding the complex interactions between these factors to develop novel ornamental plant varieties with desirable flower colors and enhanced ecological functions.
    Type of Medium: Online Resource
    ISSN: 2311-7524
    URL: Article
    DOI: 10.3390/horticulturae9040426
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2813983-5
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Metabolomic and Transcriptomic Analysis Reveal the Role of Metabolites and Genes in Modulating Flower Color of Paphiopedilum micranthum
    MDPI AG ; 2023
    In:  Plants Vol. 12, No. 10 ( 2023-05-22), p. 2058-
    Details
    In: Plants, MDPI AG, Vol. 12, No. 10 ( 2023-05-22), p. 2058-
    Abstract: Food-deceptive flowers primarily use visual signals (such as color) to mimic model plants and deceive insects into achieving pollination. Paphiopedilum micranthum is a food-deceptive orchid that has a pink labellum and two purple petals with a yellow base and has been proven to be pollinated by bumblebees. However, the chemical and molecular bases of the floral color are not well understood. We conducted targeted metabolite profiling and transcriptomic analysis to determine the color signal and its genetic basis in P. micranthum. We found that both anthocyanins and carotenoids contribute significantly to the formation of floral color that determines the color signal. Higher concentrations of anthocyanins (cyanidin and peonidin) and carotenoids (primarily lutein and zeaxanthin) were detected in the petal compared to the labellum. The upregulation of structural genes of CHS, F3′H, DFR and ANS on the anthocyanin biosynthesis pathway in petals was identified, as well as three genes of LCYE, BCH, and CCD4 on the carotenoid biosynthesis pathway. Furthermore, we discovered that three R2R3-MYBs and one bHLH transcription factors were co-expressed with the expression of different genes. These genes and transcription factors may be responsible for the spatial color difference of P. micranthum. Our study emphasizes that the color of this food-deceptive orchids is achieved through specific genes and transcription factors associated with the pigment biosynthesis pathway.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    URL: Article
    DOI: 10.3390/plants12102058
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2704341-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Effects of Bamboo Forest Type and Density on the Growth of Bletilla striata and Root Endophytic Fungi
    MDPI AG ; 2022
    In:  Diversity Vol. 14, No. 5 ( 2022-05-14), p. 391-
    Details
    In: Diversity, MDPI AG, Vol. 14, No. 5 ( 2022-05-14), p. 391-
    Abstract: Bletilla striata is a terrestrial orchid with high ornamental and medicinal values that is widely interplanted in bamboo forests. However, little is known about the effects of bamboo forest type and density on the growth of B. striata and its symbiotic relationship with root endophytic fungi. In this study, the growth state of B. striata, the community composition and diversity of its root endophytic fungal, and the fungal nutritional function were investigated in Phyllostachys edulis, P. iridescens and P. glauca forests with three densities. We found that the type and density of the bamboo forest had significant effects on the growth of B. striata, with the biomass, leaf width, root quantity and width being the highest in the low-density of the P. edulis forest. The community composition and abundance of root endophytic fungi in B. striata varied among different bamboo forests and densities, with P. edulis and P. iridescens forests dominated by Basidiomycota and Serendipita, while P. glauca prevailed by Ascomycota and Dactylonectria. The trophic modes of root endophytic fungi were also affected by forest types and densities. The abundance of symbiotroph fungi was the highest in P. edulis and P. iridescens forests and greatly varied with density gradient, and saprotrophic fungi comprised the highest proportion in the Ph. glauca forest. These results provide basic data for further research and the configuration between bamboo species and terrestrial orchids.
    Type of Medium: Online Resource
    ISSN: 1424-2818
    URL: Article
    DOI: 10.3390/d14050391
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2518137-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Root Endophytic Fungal Community and Carbon and Nitrogen Stable Isotope Patterns Differ among Bletilla Species (Orchidaceae)
    MDPI AG ; 2021
    In:  Journal of Fungi Vol. 7, No. 2 ( 2021-01-20), p. 69-
    Details
    In: Journal of Fungi, MDPI AG, Vol. 7, No. 2 ( 2021-01-20), p. 69-
    Abstract: Orchids of the genus Bletilla are well-known ornamental plants and sources of traditional medicine in Asia that rely on the symbiotic relationship with root endophytic fungi throughout their whole life cycle. However, little is known about their fungal partners, infection pattern, and pathways of carbon gain. We investigated carbon and nitrogen stable isotope patterns in different organs of three Bletilla species, identified the root endophytic fungal community composition, and determined mycorrhizal colonization rates. The three Bletilla species were comprised by a polyphyletic group which belongs to different trophic modes, such as saprotroph, pathotroph, and symbiotroph; however, the dominant species and their abundances varied among Bletilla spp. Mycorrhizal infection rates also varied among Bletilla species, with B. striata (65% ± 25%) being significantly higher than those of B. formosana (35% ± 16%) and B. ochracea (22% ± 13%). Compared with surrounding autotrophic plants, all Bletilla spp. were significantly enriched in 13C with B. striata to a significantly higher level than other two Bletilla species. Among different organs, stems had higher δ13C values, while leaves and flowers had higher δ15N and total N content values across all three species. Our results indicate that the symbiotic relationship of Bletilla and its root endophytic fungi is not strictly specific. Although mycorrhizal infection rates were highly variable, the three Bletilla species had the same infection pattern with hyphae penetrating the cortex cell by the pathway cell. Different Bletilla species have different strategies for C allocation among plant organs. These findings provide new insights into the ecological adaptation of orchids and will contribute to Bletilla germplasm conservation and sustainable utilization.
    Type of Medium: Online Resource
    ISSN: 2309-608X
    URL: Article
    DOI: 10.3390/jof7020069
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2784229-0
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Accurate Modeling and Optimization of Electromagnetic Forces in an Ironless Halbach-Type Permanent Magnet Synchronous Linear Motor
    MDPI AG ; 2023
    In:  Energies Vol. 16, No. 15 ( 2023-08-03), p. 5785-
    Details
    In: Energies, MDPI AG, Vol. 16, No. 15 ( 2023-08-03), p. 5785-
    Abstract: In order to solve the electromagnetic force optimization problem of a high-power-density ironless Halbach-type permanent magnet synchronous linear motor, this paper adopts an electromagnetic force optimization method based on magnetic field analysis, electromagnetic force modeling, and genetic algorithm optimization: Firstly, the magnetic field of the Halbach permanent magnet array is solved by the combination of the equivalent magnetization strength method and the pseudo-periodic method, which takes into account the influence of the edge effect of the secondary magnetic field, and the magnetic field of the primary winding is solved by Fourier series expansion method. Secondly, the Maxwell tensor method is used to establish the functional relationship between the electromagnetic thrust and the main structural parameters of the unilateral motor. Finally, based on the parameter sensitivity analysis of the optimized variables and the response surface calculation, the optimal combination of the optimized variables to meet the optimization objective is found by a genetic algorithm. This method of the accurate modeling and optimization of an electromagnetic force can accurately calculate the motor air gap magnetic field and electromagnetic thrust, and the optimization speed is fast, which can greatly save time. The optimization results show that, under the premise of constant input power, the unilateral average thrust of the motor is increased by 18.75%, the peak value of thrust fluctuation is decreased by 30.27%, and the results match well with the finite element results, which verifies the correctness of the optimization results of the electromagnetic force and the reasonableness of the optimization method.
    Type of Medium: Online Resource
    ISSN: 1996-1073
    URL: Article
    DOI: 10.3390/en16155785
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2437446-5
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    Joint Deployment and Coverage Path Planning for Capsule Airports with Multiple Drones
    MDPI AG ; 2023
    In:  Drones Vol. 7, No. 7 ( 2023-07-09), p. 457-
    Details
    In: Drones, MDPI AG, Vol. 7, No. 7 ( 2023-07-09), p. 457-
    Abstract: Due to the advantages of low cost and high flexibility, drones have been applied to urban surveillance, vegetation monitoring, and other fields with the need for coverage of regions. To expand UAVs’ coverage, we designed the Capsule Airport (CA) to recharge and restore drones and provide take-off and landing services. Meanwhile, the combination of drones’ coverage path planning (CPP) and the deployment of CAs is a crucial problem with few relevant studies. We propose a solution approach to the CPP problem based on selecting scanning patterns and trapezoidal decomposition. In addition, we construct a 0–1 integer programming model to minimize the cost of the distance between CAs and the scanning missions. Specifically, a solution approach based on greedy and clustering heuristics is designed to solve this problem. Furthermore, we then develop a local-search-based algorithm with the operators of CA location exchange and drone scanning mission exchange to further optimize the solution. Random instances at different sizes are used to validate the performance of proposed algorithms, through which the sensitivity analysis is conducted with some factors. Finally, a case study based on the Maolichong forest park in Changsha, China, is presented to illustrate the application of the proposed method.
    Type of Medium: Online Resource
    ISSN: 2504-446X
    URL: Article
    DOI: 10.3390/drones7070457
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2934569-8
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...