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  • 1
    Online Resource
    Online Resource
    The Novel Function of Unsymmetrical Chiral CCN Pincer Nickel Complexes as Chemotherapeutic Agents Targeting Prostate Cancer Cells
    MDPI AG ; 2022
    In:  Molecules Vol. 27, No. 10 ( 2022-05-12), p. 3106-
    Details
    In: Molecules, MDPI AG, Vol. 27, No. 10 ( 2022-05-12), p. 3106-
    Abstract: We report that the pincer nickel complexes display prostate cancer antitumor properties through inhibition of cell proliferation. Notably, they display better antitumor properties than cisplatin. Mechanistic studies reveal that these pincer nickel complexes trigger cell apoptosis, most likely due to cell cycle arrest. Interestingly, these complexes also inhibit androgen receptor (AR) and prostate-specific antigen (PSA) signaling, which are critical for prostate cancer survival and progression. Our study reveals a novel function of pincer nickel complexes as potential therapeutic drugs in prostate cancer.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    URL: Article
    DOI: 10.3390/molecules27103106
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2008644-1
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  • 2
    Online Resource
    Online Resource
    Development of a Quantitative Chromatographic Fingerprint Analysis Method for Sugar Components of Xiaochaihu Capsules Based on Quality by Design Concept
    MDPI AG ; 2022
    In:  Separations Vol. 10, No. 1 ( 2022-12-26), p. 13-
    Details
    In: Separations, MDPI AG, Vol. 10, No. 1 ( 2022-12-26), p. 13-
    Abstract: Background: Xiaochaihu capsule is composed of seven traditional Chinese medicines. The pharmacopoeia only focuses on the quantitative detection of baicalin, which cannot fully reflect the quality of the preparation. Some medium polar components were used to establish the fingerprint of Xiaochaihu capsule, but there was no report on the strong polar components. Methods: A high performance liquid chromatography-corona charged aerosol detection technology was used to establish a fingerprint analysis method for Xiaochaihu capsules following an analytical quality by design approach. Definitive screening designed experiments were used to optimize the method parameters. A stepwise regression method was used to build quantitative models. The method operable design region was calculated using the experimental error simulation method. Plackett–Burman designed experiments were carried out to test robustness. Results: The contents of four components were simultaneously determined. There were seven common peaks in the fingerprint. The common peak area accounted for 91.72%. Both fingerprint and quantitative analysis methods were validated as applicable in the methodology study. The quantitative fingerprint analysis method for sugar components can fill the gap in the detection of strong polar components in the existing methods. It provides a new technology for the comprehensive overall evaluation of Xiaochaihu capsule.
    Type of Medium: Online Resource
    ISSN: 2297-8739
    URL: Article
    DOI: 10.3390/separations10010013
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2869930-0
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  • 3
    Online Resource
    Online Resource
    Loss of Protein Function Causing Severe Phenotypes of Female-Restricted Wieacker Wolff Syndrome due to a Novel Nonsense Mutation in the ZC4H2 Gene
    MDPI AG ; 2022
    In:  Genes Vol. 13, No. 9 ( 2022-08-29), p. 1558-
    Details
    In: Genes, MDPI AG, Vol. 13, No. 9 ( 2022-08-29), p. 1558-
    Abstract: Pathogenic variants of zinc finger C4H2-type containing (ZC4H2) on the X chromosome cause a group of genetic diseases termed ZC4H2-associated rare disorders (ZARD), including Wieacker-Wolff Syndrome (WRWF) and Female-restricted Wieacker-Wolff Syndrome (WRWFFR). In the current study, a de novo c.352C 〉 T (p.Gln118*) mutation in ZC4H2 (NM_018684.4) was identified in a female neonate born with severe arthrogryposis multiplex congenita (AMC) and Pierre-Robin sequence (cleft palate and micrognathia). Plasmids containing the wild-type (WT), mutant-type (MT) ZC4H2, or GFP report gene (N) were transfected in 293T cell lines, respectively. RT-qPCR and western blot analysis showed that ZC4H2 protein could not be detected in the 293T cells transfected with MT ZC4H2. The RNA seq results revealed that the expression profile of the MT group was similar to that of the N group but differed significantly from the WT group, indicating that the c.352C 〉 T mutation resulted in the loss of function of ZC4H2. Differentially expressed genes (DEGs) enrichment analysis showed that c.352C 〉 T mutation inhibited the expression levels of a series of genes involved in the oxidative phosphorylation pathway. Subsequently, expression levels of ZC4H2 were knocked down in neural stem cells (NSCs) derived from induced pluripotent stem cells (iPSCs) by lentiviral-expressed small hairpin RNAs (shRNAs) against ZC4H2. The results also demonstrated that decreasing the expression of ZC4H2 significantly reduced the growth of NSCs by affecting the expression of genes related to the oxidative phosphorylation signaling pathway. Taken together, our results strongly suggest that ZC4H2 c.352C 〉 T (p.Gln118*) mutation resulted in the loss of protein function and caused WRWFFR.
    Type of Medium: Online Resource
    ISSN: 2073-4425
    URL: Article
    DOI: 10.3390/genes13091558
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2527218-4
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  • 4
    Online Resource
    Online Resource
    New Insights into the Relationships between Bacterial Dynamics and Water Quality of Aquaculture Systems Supplemented with Carbon Source and Biofilm Substratum
    MDPI AG ; 2021
    In:  Microorganisms Vol. 9, No. 10 ( 2021-10-18), p. 2168-
    Details
    In: Microorganisms, MDPI AG, Vol. 9, No. 10 ( 2021-10-18), p. 2168-
    Abstract: Aquaculture is crucial for achieving the FAO’s goal of a world without hunger and malnutrition. Recently, biofilm substratum has been proposed as an effective means to control waste pollution caused by excessive nutrient inputs from aquaculture, but key bacterial communities involved in the remediation remain unclear. Here we reported a freshwater mesocosm study where the addition of biofilm substrata with external carbon effectively controlled the total ammonia nitrogen and improved fish growth. 16S rRNA study and Weighted UniFrac analysis revealed that bacterial compositions were significantly different (999 permutations, p-value 〈 0.01) between the biofilm-substrata-added and biofilm-substrata-free systems. Planctomycetes were found, as key bacteria benefited from the biofilm substrata addition and exerted the major function of ammonia nitrogen control. Our study demonstrated that the addition of biofilm substrata and an external carbon source favored fish growth and improved the aquaculture environment by the formation of a unique bacteria community.
    Type of Medium: Online Resource
    ISSN: 2076-2607
    URL: Article
    DOI: 10.3390/microorganisms9102168
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2720891-6
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  • 5
    Online Resource
    Online Resource
    A Novel Transcription Factor CaSBP12 Gene Negatively Regulates the Defense Response against Phytophthora capsici in Pepper (Capsicum annuum L.)
    MDPI AG ; 2018
    In:  International Journal of Molecular Sciences Vol. 20, No. 1 ( 2018-12-22), p. 48-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 20, No. 1 ( 2018-12-22), p. 48-
    Abstract: SBP-box (Squamosa-promoter binding protein) genes are a type of plant-specific transcription factor and play important roles in plant growth, signal transduction and stress response. However, little is known about the SBP-box genes in pepper (CaSBP), especially in the process of Phytophthora capsici infection. In this study, a novel gene (CaSBP12) was selected from the CaSBP gene family, which was isolated from the pepper genome database in our previous study. The CaSBP12 gene was located in the nucleus of the cell and its silencing in the pepper plant enhanced the defense response against Phytophthora capsici infection. After inoculation with Phytophthora capsici, the root activity of the CaSBP12-silenced plants is compared to control plants, while malondialdehyde (MDA) content is compared viceversa. Additionally, the expression of defense related genes (CaPO1, CaSAR8.2, CaBPR1, and CaDEF1) in the silenced plants were induced to different degrees and the peak of CaSAR8.2 and CaBPR1 were higher than that of CaDEF1. The CaSBP12 over-expressed Nicotiana benthamiana plants were more susceptible to Phytophthora capsici infection with higher EC (electrical conductivity) and MDA contents as compared to the wild-type. The relative expression of defense related genes (NbDEF, NbNPR1, NbPR1a, and NbPR1b) in transgenic and wild-type Nicotiana benthamiana plants were induced, especially the NbPR1a and NbPR1b. In conclusion, these results indicate that CaSBP12 gene negative regulates the defense response against Phytophthora capsici infection which suggests their potentially significant role in plant defense. To our knowledge, this is the first report on CaSBP gene which negative regulate defense response.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms20010048
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Tetrocarcin Q, a New Spirotetronate with a Unique Glycosyl Group from a Marine-Derived Actinomycete Micromonospora carbonacea LS276
    MDPI AG ; 2018
    In:  Marine Drugs Vol. 16, No. 2 ( 2018-02-24), p. 74-
    Details
    In: Marine Drugs, MDPI AG, Vol. 16, No. 2 ( 2018-02-24), p. 74-
    Abstract: A new spirotetronate glycoside tetrocarcin Q (1) and six known analogues tetrocarcin A (2), AC6H (3), tetrocarcin N (4), tetrocarcin H (5), arisostatin A (6), and tetrocarcin F1 (7) were isolated from the fermentation broth of the marine-derived actinomycete Micromonospora carbonacea LS276. Their chemical structures were established on the basis of 1D- and 2D-NMR spectroscopy, as well as HR-ESI-MS analysis. The absolute configurations of their stereogenic carbons were determined by circular dichroism (CD) analysis. Compound 1 possesses 2-deoxy-allose, which is a unique sugar type at the C-9 position. This type has not been found in the previously reported spirotetronate glycosides. Compound 1 displayed moderate antibacterial activity against Bacillus subitlis ATCC 63501 with minimum inhibitory concentration (MIC) value of 12.5 μM.
    Type of Medium: Online Resource
    ISSN: 1660-3397
    URL: Article
    DOI: 10.3390/md16020074
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2175190-0
    SSG: 15,3
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  • 7
    Online Resource
    Online Resource
    CaHsfA1d Improves Plant Thermotolerance via Regulating the Expression of Stress- and Antioxidant-Related Genes
    MDPI AG ; 2020
    In:  International Journal of Molecular Sciences Vol. 21, No. 21 ( 2020-11-08), p. 8374-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 21, No. 21 ( 2020-11-08), p. 8374-
    Abstract: Heat shock transcription factor (Hsf) plays an important role in regulating plant thermotolerance. The function and regulatory mechanism of CaHsfA1d in heat stress tolerance of pepper have not been reported yet. In this study, phylogenetic tree and sequence analyses confirmed that CaHsfA1d is a class A Hsf. CaHsfA1d harbored transcriptional function and predicted the aromatic, hydrophobic, and acidic (AHA) motif mediated function of CaHsfA1d as a transcription activator. Subcellular localization assay showed that CaHsfA1d protein is localized in the nucleus. The CaHsfA1d was transcriptionally up-regulated at high temperatures and its expression in the thermotolerant pepper line R9 was more sensitive than that in thermosensitive pepper line B6. The function of CaHsfA1d under heat stress was characterized in CaHsfA1d-silenced pepper plants and CaHsfA1d-overexpression Arabidopsis plants. Silencing of the CaHsfA1d reduced the thermotolerance of the pepper, while CaHsfA1d-overexpression Arabidopsis plants exhibited an increased insensitivity to high temperatures. Moreover, the CaHsfA1d maintained the H2O2 dynamic balance under heat stress and increased the expression of Hsfs, Hsps (heat shock protein), and antioxidant gene AtGSTU5 (glutathione S-transferase class tau 5) in transgenic lines. Our findings clearly indicate that CaHsfA1d improved the plant thermotolerance via regulating the expression of stress- and antioxidant-related genes.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms21218374
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 8
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    Online Resource
    CaFtsH06, A Novel Filamentous Thermosensitive Protease Gene, Is Involved in Heat, Salt, and Drought Stress Tolerance of Pepper (Capsicum annuum L.)
    MDPI AG ; 2021
    In:  International Journal of Molecular Sciences Vol. 22, No. 13 ( 2021-06-28), p. 6953-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 22, No. 13 ( 2021-06-28), p. 6953-
    Abstract: Harsh environmental factors have continuous negative effects on plant growth and development, leading to metabolic disruption and reduced plant productivity and quality. However, filamentation temperature-sensitive H protease (FtsH) plays a prominent role in helping plants to cope with these negative impacts. In the current study, we examined the transcriptional regulation of the CaFtsH06 gene in the R9 thermo-tolerant pepper (Capsicum annuum L.) line. The results of qRT-PCR revealed that CaFtsH06 expression was rapidly induced by abiotic stress treatments, including heat, salt, and drought. The CaFtsH06 protein was localized to the mitochondria and cell membrane. Additionally, silencing CaFtsH06 increased the accumulation of malonaldehyde content, conductivity, hydrogen peroxide (H2O2) content, and the activity levels of superoxide dismutase and superoxide (·O2−), while total chlorophyll content decreased under these abiotic stresses. Furthermore, CaFtsH06 ectopic expression enhanced tolerance to heat, salt, and drought stresses, thus decreasing malondialdehyde, proline, H2O2, and ·O2− contents while superoxide dismutase activity and total chlorophyll content were increased in transgenic Arabidopsis. Similarly, the expression levels of other defense-related genes were much higher in the transgenic ectopic expression lines than WT plants. These results suggest that CaFtsH06 confers abiotic stress tolerance in peppers by interfering with the physiological indices through reducing the accumulation of reactive oxygen species, inducing the activities of stress-related enzymes and regulating the transcription of defense-related genes, among other mechanisms. The results of this study suggest that CaFtsH06 plays a very crucial role in the defense mechanisms of pepper plants to unfavorable environmental conditions and its regulatory network with other CaFtsH genes should be examined across variable environments.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms22136953
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 9
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    Online Resource
    Self-Heating Graphene Nanocomposite Bricks: A Case Study in China
    MDPI AG ; 2020
    In:  Materials Vol. 13, No. 3 ( 2020-02-05), p. 714-
    Details
    In: Materials, MDPI AG, Vol. 13, No. 3 ( 2020-02-05), p. 714-
    Abstract: In cold climate regions, the energy associated with indoor heating constitutes a large portion of energy consumption. Increasing energy utilization efficiency is critically important for both economic and environmental reasons. Directly converting electrical energy to thermal energy using joule heating construction elements can save energy and investment to the water pipelines which have been extensively used for indoor heating in China. The fired brick has been extensively used to make pavements, walls and other masonry. Taking advantage of the high dispersion quality of graphene oxide (GO) in water, as well as the firing process used to make fired bricks, graphene nanocomposite bricks with excellent electrical properties and improved mechanical performance were prepared in China. The compressive strength of the bricks showed a substantial increase from 3.15 MPa to 7.21 MPa when GO concentration was 0.1 wt.%. Through applying 5 volts of electrical field within 5 minutes, the nanocomposites can be heated from room temperature to 60 °C, 110 °C and 160 °C for the nanocomposite bricks with graphene concentration of 3 wt.%, 4 wt.% and 5 wt.%, respectively, due to the extremely low percolation threshold (~0.5 wt.%) and high conductivity (10 Ω·cm at 1 wt.%). The sheets were connected more tightly when the GO content was increased. The thermal efficiency can reach up to 88% based on the applied voltage, measured resistance and temperature rise curves.
    Type of Medium: Online Resource
    ISSN: 1996-1944
    URL: Article
    DOI: 10.3390/ma13030714
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2487261-1
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  • 10
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    Online Resource
    Effects of Curing Conditions on the MECHANICAL and Microstructural Properties of Ultra-High-Performance Concrete (UHPC) Incorporating Iron Tailing Powder
    MDPI AG ; 2021
    In:  Materials Vol. 14, No. 1 ( 2021-01-04), p. 215-
    Details
    In: Materials, MDPI AG, Vol. 14, No. 1 ( 2021-01-04), p. 215-
    Abstract: It has been reported that iron tailing powder (ITP) has the potential to partially replace cement to prepare ultra-high-performance concrete (UHPC). However, the reactivity of ITP particles in concrete largely depends on the curing method. This study investigates the effects of curing conditions on the mechanical and microstructural properties of UHPC containing ITP. To achieve this objective, three research tasks are conducted, including (1) preparing seven concrete formulations by introducing ITP; (2) characterizing their mechanical performance under different curing regimes; and (3) analyzing their microstructure by XRD patterns, FTIR analysis, and SEM observation. The experimental results show that there is an optimum ITP dosage (15%) for their application. The concrete with 15% ITP under standard curing obtains 94.3 MPa at 7 days, their early-age strength could be even further increased by ~30% (warm-water curing) and ~35% (steamed curing). The steam curing regime stimulates the activity of ITP and refines the microstructure. This study demonstrates the potential of replacing Portland cement with ITP in UHPC production.
    Type of Medium: Online Resource
    ISSN: 1996-1944
    URL: Article
    DOI: 10.3390/ma14010215
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2487261-1
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