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  • 1
    In: Plants, MDPI AG, Vol. 10, No. 9 ( 2021-09-09), p. 1870-
    Abstract: Centaurium erythraea (centaury) is a medicinal plant with exceptional developmental plasticity in vitro and vigorous, often spontaneous, regeneration via shoot organogenesis and somatic embryogenesis, during which arabinogalactan proteins (AGPs) play an important role. AGPs are highly glycosylated proteins belonging to the super family of O-glycosylated plant cell surface hydroxyproline-rich glycoproteins (HRGPs). HRGPs/AGPs are intrinsically disordered and not well conserved, making their homology-based mining ineffective. We have applied a recently developed pipeline for HRGP/AGP mining, ragp, which is based on machine learning prediction of proline hydroxylation, to identify HRGP sequences in centaury transcriptome and to classify them into motif and amino acid bias (MAAB) classes. AGP sequences with low AG glycomotif representation were also identified. Six members of each of the three AGP subclasses, fasciclin-like AGPs, receptor kinase-like AGPs and AG peptides, were selected for phylogenetic and expression analyses. The expression of these 18 genes was recorded over 48 h following leaf mechanical wounding, as well as in 16 tissue samples representing plants from nature, plants cultivated in vitro, and developmental stages during shoot organogenesis and somatic embryogenesis. None of the selected genes were upregulated during both wounding recovery and regeneration. Possible functions of AGPs with the most interesting expression profiles are discussed.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2704341-1
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  • 2
    In: Plants, MDPI AG, Vol. 10, No. 1 ( 2021-01-14), p. 154-
    Abstract: Aquaporins comprise a large group of transmembrane proteins responsible for water transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins, nothing is known about this protein family in Impatiens walleriana, a commercially important horticultural plant, which is sensitive to drought stress. In the present study, attention is given to the molecular characterization of aquaporins in I. walleriana and their expression during drought stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein. Drought stress and recovery treatment affected the aquaporins expression in I. walleriana leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively. Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins expanded knowledge about plant aquaporins, while its expression during drought and recovery contributed to I. walleriana drought tolerance mechanisms and re-acclimation.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2704341-1
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  • 3
    In: Plants, MDPI AG, Vol. 9, No. 11 ( 2020-11-17), p. 1589-
    Abstract: Impatiens walleriana is a valued ornamental plant sensitive to drought stress. We investigated whether the foliar application of 2mM salicylic acid (SA) can protect potted I. walleriana plants from drought stress. The plants were divided into: watered plants, drought-stressed plants, watered plants treated with SA and drought-stressed plants treated with SA. The number of flowers and flower buds, relative water content (RWC), contents of malondialdehyde (MDA) and proline (Pro) and the activities of superoxide dismutases, catalases and peroxidases were recorded at different time points. Three dehydrin sequences were identified in de novo assembled leaf transcriptome: IwDhn1, IwDhn2.1 and IwDhn2.2. Drought stress caused wilting, floral abortion, reduction of RWC and increased MDA—an indicator of lipid peroxidation. In response to drought, Impatiens accumulated Pro and induced chloroplastic Cu/ZnSOD and two peroxidase isoforms. The most remarkable drought response was strong induction of IwDhn2.1 and IwDhn2.2. Rehydration restored RWC, Pro level, Cu/ZnSOD activity and dehydrins expression in drought-stressed plants approximately to the values of watered plants.SA had ameliorating effects on plants exposed to drought, including prevention of wilting, preservation of RWC, increased Pro accumulation, modulation of antioxidative activities and remarkable decrease of lipid peroxidation, but without effects on flowers’ preservation.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2704341-1
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  • 4
    In: Atmosphere, MDPI AG, Vol. 14, No. 1 ( 2023-01-03), p. 104-
    Abstract: The Western Balkans (WB) region is highly prone to water erosion processes, and therefore, the estimation of rainfall erosivity (R-factor) is essential for understanding the complex relationships between hydro-meteorological factors and soil erosion processes. The main objectives of this study are to (1) estimate the spatial-temporal distribution R-factor across the WB region by applying the RUSLE and RUSLE2 methodology with data for the period between 1991 and 2020 and (2) apply cluster analysis to identify places of high erosion risk, and thereby offer a means of targeting suitable mitigation measures. To assess R-factor variability, the ERA5 reanalysis hourly data (0.25° × 0.25° spatial resolution) comprised 390 grid points were used. The calculations were made on a decadal resolution (i.e., for the 1990s, the 2000s, and the 2010s), as well as for the whole study period (1991–2020). In order to reveal spatial patterns of rainfall erosivity, a k-means clustering algorithm was applied. Visualization and mapping were performed in python using the Matplotlib, Seaborn, and Cartopy libraries. Hourly precipitation intensity and monthly precipitation totals exhibited pronounced variability over the study area. High precipitation values were observed in the SW with a 〉 0.3 mm h−1 average, while the least precipitation was seen in the Pannonian Basin and far south (Albanian coast), where the mean intensity was less than an average of 0.1 mm h−1. R-factor variability was very high for both the RUSLE and RUSLE2 methods. The mean R-factor calculated by RUSLE2 was 790 MJ mm ha−1·h−1·yr−1, which is 58% higher than the mean R-factor obtained from RUSLE (330 MJ mm ha−1·h−1·yr−1). The analysis of the R-factor at decadal timescales suggested a rise of 14% in the 2010s. The k-means algorithm for both the RUSLE and RUSLE2 methods implies better spatial distribution in the case of five clusters (K = 5) regarding the R-factor values. The rainfall erosivity maps presented in this research can be seen as useful tools for the assessment of soil erosion intensity and erosion control works, especially for agriculture and land use planning. Since the R-factor is an important part of soil erosion models (RUSLE and RUSLE2), the results of this study can be used as a guide for soil control works, landscape modeling, and suitable mitigation measures on a regional scale.
    Type of Medium: Online Resource
    ISSN: 2073-4433
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2605928-9
    SSG: 23
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  • 5
    In: Diversity, MDPI AG, Vol. 15, No. 9 ( 2023-08-25), p. 962-
    Abstract: This work presents refined, updated subregional and regional non-indigenous species (NIS) inventories for the Mediterranean Sea, validated by national and taxonomic experts, with species records observed until December 2020. These datasets will be used as the baselines for the implementation of the Integrated Monitoring and Assessment Programme for the Mediterranean (IMAP) and the Mediterranean Quality Status Report 2023. In total, 1006 non-indigenous species have been found in Mediterranean marine and brackish waters. The highest numbers of NIS were observed in Israel, Türkiye, Lebanon and Italy. Approximately 45 species were categorized as data deficient, either due to lack of consensus on their alien status or the validity of their identification. Polychaeta, Foraminifera and macroalgae were the groups with the highest numbers of controversial species. There was a general increase in the yearly rate of new NIS introductions after the late 1990s, which appears to be slowing down in the last decade, but this may be confounded by reporting lags and differential research efforts. Between 1970 and 2020 there has been a steep increase in the proportion of shared species present throughout all four Mediterranean subregions, which are predominantly transported via shipping and recreational boating. While Lessepsian species are gradually spreading westwards and northwards, there is still a considerable invasion debt accumulating in the eastern and central Mediterranean.
    Type of Medium: Online Resource
    ISSN: 1424-2818
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2518137-3
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