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  • 1
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 23, No. 11 ( 2022-05-25), p. 5941-
    Abstract: Hybrid rice technology has been used for more than 50 years, and eating and cooking quality (ECQ) has been a major focus throughout this period. Waxy (Wx) and alkaline denaturation (ALK) genes have received attention owing to their pivotal roles in determining rice characteristics. However, despite significant effort, the ECQ of restorer lines (RLs) has changed very little. By contrast, obvious changes have been seen in inbred rice varieties (IRVs), and the ECQ of IRVs is influenced by Wx, which reduces the proportion of Wxa and increases the proportion of Wxb, leading to a decrease in amylose content (AC) and an increase in ECQ. Meanwhile, ALK is not selected in the same way. We investigated Wx alleles and AC values of sterile lines of female parents with the main mating combinations in widely used areas. The results show that almost all sterile lines were Wxa-type with a high AC, which may explain the low ECQ of hybrid rice. Analysis of hybrid rice varieties and RLs in the last 5 years revealed serious homogenisation among hybrid rice varieties.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 2
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 19, No. 11 ( 2018-11-04), p. 3460-
    Abstract: Seed germination, a pivotal process in higher plants, is precisely regulated by various external and internal stimuli, including brassinosteroid (BR) and gibberellin (GA) phytohormones. The molecular mechanisms of crosstalk between BRs and GAs in regulating plant growth are well established. However, whether BRs interact with GAs to coordinate seed germination remains unknown, as do their common downstream targets. In the present study, 45 differentially expressed proteins responding to both BR and GA deficiency were identified using isobaric tags for relative and absolute quantification (iTRAQ) proteomic analysis during seed germination. The results indicate that crosstalk between BRs and GAs participates in seed germination, at least in part, by modulating the same set of responsive proteins. Moreover, most targets exhibited concordant changes in response to BR and GA deficiency, and gene ontology (GO) indicated that most possess catalytic activity and are involved in various metabolic processes. Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) analysis was used to construct a regulatory network of downstream proteins mediating BR- and GA-regulated seed germination. The mutation of GRP, one representative target, notably suppressed seed germination. Our findings not only provide critical clues for validating BR–GA crosstalk during rice seed germination, but also help to optimise molecular regulatory networks.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 3
    In: Sustainability, MDPI AG, Vol. 12, No. 5 ( 2020-03-09), p. 2094-
    Abstract: Soil organic carbon (SOC) concentration is closely related to soil quality and climate change. The objectives of this study were to estimate the effects of contemporary land use on SOC concentrations at 0–20 cm depths, and to investigate the dynamics of SOC in paddy-field soil and dry-land soil after their conversion from natural wetlands (20 and 30 years ago). We investigated the dissolved organic carbon (DOC), light fraction organic carbon (LFOC), heavy fraction organic carbon (HFOC), and other soil properties (i.e., moisture content, bulk density, pH, clay, sand, silt, available phosphorous, light fraction nitrogen, and heavy fraction nitrogen) in natural wetlands, constructed wetlands, fishponds, paddy fields, and soybean fields. The results indicated that the content of DOC increased 17% in constructed wetland and decreased 39% in fishponds, and the content of HFOC in constructed wetland and fishponds increased 50% and 8%, respectively, compared with that in natural wetlands at 0–20 cm. After the conversion of a wetland, the content of HFOC increased 72% in the paddy fields and decreased 62% in the dry land, while the content of DOC and LFOC decreased in both types. In the paddy fields, LFOC and HFOC content in the topmost 0.2 m of the soil layer was significantly higher compared to the layer below (from 0.2 to 0.6 m), and there were no significant differences observed in the dry land. The findings suggest that the paddy fields can sequester organic carbon through the accumulation of HFOC. However, the HFOC content decreased 22% after 10 years of cultivation with the decrease of clay content, indicating that paddy fields need to favor clay accumulation for the purpose of enhancing carbon sequestration in the paddy fields.
    Type of Medium: Online Resource
    ISSN: 2071-1050
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2518383-7
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  • 4
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 22, No. 4 ( 2021-02-07), p. 1661-
    Abstract: Seed dormancy and germination are key events in plant development and are critical for crop production, and defects in seed germination or the inappropriate release of seed dormancy cause substantial losses in crop yields. Rice is the staple food for more than half of the world’s population, and preharvest sprouting (PHS) is one of the most severe problems in rice production, due to a low level of seed dormancy, especially under warm and damp conditions. Therefore, PHS leads to yield loss and a decrease in rice quality and vitality. We reveal that mutation of OsbZIP09 inhibited rice PHS. Analysis of the expression of OsbZIP09 and its encoded protein sequence and structure indicated that OsbZIP09 is a typical bZIP transcription factor that contains conserved bZIP domains, and its expression is induced by ABA. Moreover, RNA sequencing (RNA-seq) and DNA affinity purification sequencing (DAP-seq) analyses were performed and 52 key direct targets of OsbZIP09 were identified, including OsLOX2 and Late Embryogenesis Abundant (LEA) family genes, which are involved in controlling seed germination. Most of these key targets showed consistent changes in expression in response to abscisic acid (ABA) treatment and OsbZIP09 mutation. The data characterize a number of key target genes that are directly regulated by OsbZIP09 and contribute to revealing the molecular mechanism that underlies how OsbZIP09 controls rice seed germination.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 5
    In: Materials, MDPI AG, Vol. 10, No. 8 ( 2017-08-15), p. 946-
    Type of Medium: Online Resource
    ISSN: 1996-1944
    Language: English
    Publisher: MDPI AG
    Publication Date: 2017
    detail.hit.zdb_id: 2487261-1
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  • 6
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 20, No. 19 ( 2019-10-03), p. 4908-
    Abstract: Leaf angle is a key parameter that determines plant architecture and crop yield. Hormonal crosstalk involving brassinosteroid (BR) plays an essential role in leaf angle regulation in cereals. In this study, we investigated whether abscisic acid (ABA), an important stress-responsive hormone, co-regulates lamina joint inclination together with BR, and, if so, what the underlying mechanism is. Therefore, lamina joint inclination assay and RNA sequencing (RNA-Seq) analysis were performed here. ABA antagonizes the promotive effect of BR on leaf angle. Hundreds of genes responsive to both hormones that are involved in leaf-angle determination were identified by RNA-Seq and the expression of a gene subset was confirmed using quantitative real-time PCR (qRT-PCR). Results from analysis of rice mutants or transgenic lines affected in BR biosynthesis and signaling indicated that ABA antagonizes the effect of BR on lamina joint inclination by targeting the BR biosynthesis gene D11 and BR signaling genes GSK2 and DLT, thus forming a multi-level regulatory module that controls leaf angle in rice. Taken together, our findings demonstrate that BR and ABA antagonistically regulate lamina joint inclination in rice, thus contributing to the elucidation of the complex hormonal interaction network that optimizes leaf angle in rice.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 7
    In: Remote Sensing, MDPI AG, Vol. 14, No. 19 ( 2022-10-09), p. 5011-
    Abstract: Vegetation dynamics are often affected by climate variability, but the past state of vegetation has a non-negligible impact on current vegetation growth. However, seasonal differences in the effects of these drivers on vegetation growth remain unclear, particularly in ecologically fragile areas. We used the normalized difference vegetation index (NDVI), gross primary productivity (GPP), and leaf area index (LAI) to describe the vegetation dynamic in the upper and middle reaches of the Yellow River basin (YRB). Three active vegetation growing seasons (early, peak, and late) were defined based on phenological metrics. In light of three vegetation indicators and the climatic data, we identified the correlation between the inter-annual variation of vegetation growth in the three sub-seasons. Then, we quantified the contributions of climate variability and the vegetation growth carryover (VGC) effect on seasonal vegetation greening between 2000–2019. Results showed that both the vegetation coverage and productivity in the study area increased over a 20-year period. The VGC effect dominated vegetation growth during the three active growing seasons, and the effect increased from early to late growing season. Vegetation in drought regions was found to generally have a stronger vegetation carryover ability, implying that negative disturbances might have severer effects on vegetation in these areas. The concurrent seasonal precipitation was another positive driving factor of vegetation greening. However, sunshine duration, including its immediate and lagged impacts, had a negative effect on vegetation growth. In addition, the VGC effect can sustain into the second year. The VGC effect showed that initial ecological restoration and sustainable conservation would promote vegetation growth and increase vegetation productivity. This study provides a comprehensive perspective on understanding the climate–vegetation interactions on a seasonal scale, which helps to accurately predict future vegetation dynamics over time in ecologically fragile areas.
    Type of Medium: Online Resource
    ISSN: 2072-4292
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2513863-7
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  • 8
    In: Sensors, MDPI AG, Vol. 18, No. 5 ( 2018-04-24), p. 1314-
    Type of Medium: Online Resource
    ISSN: 1424-8220
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2052857-7
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  • 9
    In: Molecules, MDPI AG, Vol. 21, No. 4 ( 2016-04-21), p. 514-
    Type of Medium: Online Resource
    ISSN: 1420-3049
    Language: English
    Publisher: MDPI AG
    Publication Date: 2016
    detail.hit.zdb_id: 2008644-1
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  • 10
    In: Agronomy, MDPI AG, Vol. 13, No. 5 ( 2023-04-25), p. 1210-
    Abstract: Soilless cultivation has increased in China. Like vegetables or ornamental plants, tomatoes can be cultivated in soilless culture systems. Fusarium wilt (FW) is an economic tomato disease. The nature and amount of volatile organic compounds in Trichoderma asperellum PT-15 were investigated, and 6-pentyl-α-pyrone (6-PP) was detected. Furthermore, the effect of 6-PP on Fusarium oxysporum HF-26 was evaluated. Results revealed that 25 mg/L 6-PP was the optimal concentration inhibiting F. oxysporum HF-26 and that the content of fusaric acid decreased considerably compared to that of the control. FUB1, FUB4, and FUB10 toxin synthesis and transport genes were downregulated. Additionally, VelA, velB, and LaeA genes were downregulated, reducing F. oxysporum mycelial growth and hyphae formation. 6-PP was added to the soilless culture solution in a greenhouse experiment. The results showed that an antifungal–nutrient solution containing 25 mg/L 6-PP significantly suppressed FW with 70.71% efficacy and a 27.23% disease index (DI), which were higher efficacy and lower DI than that of the control. Furthermore, treatment with an antifungal–nutrient solution containing 6-PP increased the levels of defence enzymes 24 h post-inoculation (hpi) compared with those at other time periods. The relative expression levels of the PR1, NPR1, PR2, and PR5 genes were considerably upregulated at 24 hpi.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2607043-1
    SSG: 23
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