GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Magnetic SERS Strip Based on 4-mercaptophenylboronic Acid-Modified Fe3O4@Au for Active Capture and Simultaneous Detection of Respiratory Bacteria
    MDPI AG ; 2023
    In:  Biosensors Vol. 13, No. 2 ( 2023-01-31), p. 210-
    Details
    In: Biosensors, MDPI AG, Vol. 13, No. 2 ( 2023-01-31), p. 210-
    Abstract: The rapid diagnosis and detection of respiratory bacteria at the early stage can effectively control the epidemic spread and bacterial infection. Here, we designed a rapid, ultrasensitive, and quantitative lateral flow immunoassay (LFA) strip for simultaneous detection of respiratory bacteria S. aureus and S. pneumoniae. In this assay, the surface enhanced Raman scattering (SERS) tags were designed through combining magnetite Raman enhancement nanoparticle Fe3O4@Au/DTNB and recognition element 4-mercaptophenylboronic acid (4-MPBA). Further, 4-MPBA could capture multiple bacteria in a complex environmental solution. Based on the strategies, Fe3O4@Au/DTNB-mediated magnetic enrichment and 4-MPBA-mediated universal capture capabilities improved the detection sensitivity, the limits of detection for S. aureus and S. pneumoniae were as low as 8 and 13 CFU mL−1, respectively, which were more sensitive than those of colloidal gold method. The Fe3O4@Au/DTNB/Au/4-MPBA-LFA also exhibited good reproducibility, excellent specificity, and high recovery rates in sputum samples, indicating its potential application in the detection of respiratory bacteria samples.
    Type of Medium: Online Resource
    ISSN: 2079-6374
    URL: Article
    DOI: 10.3390/bios13020210
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2662125-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Research on the Dynamic Mechanism of Technological Innovation Diffusion in Enterprise Communities Based on a Predation Diffusion Model
    MDPI AG ; 2023
    In:  Axioms Vol. 12, No. 9 ( 2023-08-30), p. 847-
    Details
    In: Axioms, MDPI AG, Vol. 12, No. 9 ( 2023-08-30), p. 847-
    Abstract: In order to study the dynamic mechanism of the impact of technological innovation diffusion on enterprise population networks, a corresponding relationship between enterprise population networks and predatory models was established based on a predatory model. Without considering the impact of technological innovation diffusion, the stability of the enterprise population network was analyzed, and the results showed that it has the characteristic of local asymptotic stability at a positive equilibrium point. Considering the influence of technological innovation diffusion, the stability of the enterprise population network becomes complex, and its stability at the positive equilibrium point is also affected by the eigenvalue of the Laplacian matrix and technological innovation diffusion coefficient. The simulation experimental results indicate that in addition to the influence of technological innovation diffusion coefficient, the connection probability density of enterprise population networks has an important impact on stability. Only when the connection probability density is very small can the enterprise population network resist the impact of technological innovation diffusion and maintain stability.
    Type of Medium: Online Resource
    ISSN: 2075-1680
    URL: Article
    DOI: 10.3390/axioms12090847
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2661511-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Detecting the Hydrogen Bond Cooperativity in a Protein β-Sheet by H/D Exchange
    MDPI AG ; 2022
    In:  International Journal of Molecular Sciences Vol. 23, No. 23 ( 2022-11-26), p. 14821-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 23, No. 23 ( 2022-11-26), p. 14821-
    Abstract: The hydrogen bond (H-bond) cooperativity in the β-sheet of GB3 is investigated by a NMR hydrogen/deuterium (H/D) exchange method. It is shown that the weakening of one backbone N–H…O=C H-bond between two β-strands, β1 and β2, due to the exchange of NH to ND of the H-bond donor in β1, perturbs the chemical shift of 13Cα, 13Cβ, 1Hα, 1HN, and 15N of the H-bond acceptor and its following residue in β2. Quantum mechanical calculations suggest that the -H-bond chemical shift isotope effect is caused by the structural reorganization in response to the H-bond weakening. This structural reorganization perturbs four neighboring H-bonds, with three being weaker and one being stronger, indicating that three H-bonds are cooperative and one is anticooperative with the perturbed H-bond. The sign of the cooperativity depends on the relative position of the H-bonds. This H-bond cooperativity, which contributes to β-sheet stability overall, can be important for conformational coupling across the β-sheet.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms232314821
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2019364-6
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Identification of a Novel Linear B-Cell Epitope on the Nucleocapsid Protein of Porcine Deltacoronavirus
    MDPI AG ; 2020
    In:  International Journal of Molecular Sciences Vol. 21, No. 2 ( 2020-01-19), p. 648-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 21, No. 2 ( 2020-01-19), p. 648-
    Abstract: Porcine deltacoronavirus (PDCoV), first identified in 2012, is a swine enteropathogen now found in many countries. The nucleocapsid (N) protein, a core component of PDCoV, is essential for virus replication and is a significant candidate in the development of diagnostics for PDCoV. In this study, monoclonal antibodies (mAbs) were generated and tested for reactivity with three truncations of the full protein (N1, N2, N3) that contained partial overlaps; of the five monoclonals chosen tested, each reacted with only the N3 truncation. The antibody designated 4E88 had highest binding affinity with the N protein and was chosen for in-depth examination. The 4E88 epitope was located to amino acids 308-AKPKQQKKPKK-318 by testing the 4E88 monoclonal for reactivity with a series of N3 truncations, then the minimal epitope, 309-KPKQQKKPK-317 (designated EP-4E88), was pinpointed by testing the 4E88 monoclonal for reactivity with a series of synthetic peptides of this region. Homology analysis showed that the EP-4E88 sequence is highly conserved among PDCoV strains, and also shares high similarity with sparrow coronavirus (HKU17), Asian leopard cat coronavirus (ALCCoV), quail coronavirus (UAE-HKU30), and sparrow deltacoronavirus (SpDCoV). Of note, the PDCoV EP-4E88 sequence shared very low similarity ( 〈 22.2%) with other porcine coronaviruses (PEDV, TGEV, PRCV, SADS-CoV, PHEV), demonstrating that it is an epitope that can be used for distinguishing PDCoV and other porcine coronavirus. 3D structural analysis revealed that amino acids of EP-4E88 were in close proximity and may be exposed on the surface of the N protein.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms21020648
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2019364-6
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Research on Detection of Ultra-Low Concentration Anthrax Protective Antigen Using Graphene Field-Effect Transistor Biosensor
    MDPI AG ; 2023
    In:  Sensors Vol. 23, No. 13 ( 2023-06-22), p. 5820-
    Details
    In: Sensors, MDPI AG, Vol. 23, No. 13 ( 2023-06-22), p. 5820-
    Abstract: Background: Protective antigen (PA) is an important biomarker for the early diagnosis of anthrax, and the accurate detection of protective antigen under extremely low concentration conditions has always been a hot topic in the biomedical field. To complete the diagnosis of anthrax in a timely manner, it is necessary to detect PA at extremely low concentrations, as the amount of PA produced in the early stage of anthrax invasion is relatively small. Graphene field-effect transistor (Gr−FET) biosensors are a new type of material for preparing biosensors, with the advantages of a short detection time and ultra-low detection limit. Methods: The effect of different concentrations of diluents on the affinity of PA monoclonal antibodies was determined via an ELISA experiment. Combined with the Debye equation, 0.01 × PBS solution was finally selected as the diluent for the experiment. Then, a PA monoclonal antibody was selected as the bio-recognition element to construct a Gr−FET device based on CVD-grown graphene, which was used to detect the concentration of PA while recording the response time, linear range, detection limit, and other parameters. Results: The experimental results showed that the biosensor could quickly detect PA, with a linear range of 10 fg/mL to 100 pg/mL and a detection limit of 10 fg/mL. In addition, the biosensor showed excellent specificity and repeatability. Conclusions: By constructing a Gr−FET device based on CVD-grown graphene and selecting a PA monoclonal antibody as the bio-recognition element, a highly sensitive, specific, and repeatable Gr−FET biosensor was successfully prepared for detecting extremely low concentrations of anthrax protective antigen (PA). This biosensor is expected to have a wide range of applications in clinical medicine and biological safety monitoring.
    Type of Medium: Online Resource
    ISSN: 1424-8220
    URL: Article
    DOI: 10.3390/s23135820
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2052857-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Research and Design of LC Series Resonant Wireless Power Transfer System with Modulation Control Method for Supercapacitor Charging in Linear Motion Systems
    MDPI AG ; 2022
    In:  Energies Vol. 15, No. 18 ( 2022-09-15), p. 6739-
    Details
    In: Energies, MDPI AG, Vol. 15, No. 18 ( 2022-09-15), p. 6739-
    Abstract: With the hot topic of “Carbon Neutrality”, energy efficiency and saving practices such as reducing fuel consumption, vigorously advocating new energy power and modern rail are now becoming the main research topics of energy conversion technologies. Supercapacitors, with their ability of higher power density, fast charging, and instantaneous high current output, have become an indispensable energy storage element in modern traction systems for modern rail. This proposal introduced wireless power transfer technologies by using LC series resonant technology for charging the supercapacitors. To match the voltage and current level of the supercapacitor, a four-switch buck-boost converter was applied on the secondary side of the load-matching converter. To regulate the wireless transfer power and charging power of the supercapacitor, the active modulation control method was introduced on both the primary and secondary sides of the transfer system. On the primary side, the power is controlled by controlling the current in resonant inductance through the phase shift control method, while on the secondary side, the charging power is controlled by regulating the input voltage of the four-switch buck-boost converter followed by inductance current control. The theoretical analysis under phase shift mode for the primary side and pulse width modulation for a four-switch buck-boost converter with a supercapacitor load (voltage source) were proposed in detail, and the state-space model of the load matching converter was established for controller design to obtain precise voltage and current control. Both open loop and closed loop simulation models were built in the MATLAB/SIMULINK environment, and simulations were carried out to evaluate the system characteristics and control efficiency. The experimental platform was established based on a dsPIC33FJ64GS606 digital controller. Experiments were carried out, and the results successfully verified the effectiveness of the system.
    Type of Medium: Online Resource
    ISSN: 1996-1073
    URL: Article
    DOI: 10.3390/en15186739
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2437446-5
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    Long Time Behavior and Global Dynamics of Simplified Von Karman Plate Without Rotational Inertia Driven by White Noise
    MDPI AG ; 2018
    In:  Symmetry Vol. 10, No. 8 ( 2018-08-01), p. 315-
    Details
    In: Symmetry, MDPI AG, Vol. 10, No. 8 ( 2018-08-01), p. 315-
    Abstract: Without the assumption that the coefficient of weak damping is large enough, the existence of the global random attractors for simplified Von Karman plate without rotational inertia driven by either additive white noise or multiplicative white noise are proved. Instead of the classical splitting method, the techniques to verify the asymptotic compactness rely on stabilization estimation of the system. Furthermore, a clear relationship between in-plane components of the external force that act on the edge of the plate and the expectation of radius of the global random attractors can be obtained from the theoretical results. Based on the relationship between global random attractor and random probability invariant measure, the global dynamics of the plates are analyzed numerically. With increasing the in-plane components of the external force that act on the edge of the plate, global D-bifurcation, secondary global D-bifurcation and complex local dynamical behavior occur in motion of the system. Moreover, increasing the intensity of white noise leads to the dynamical behavior becoming simple. The results on global dynamics reveal that random snap-through which seems to be a complex dynamics intuitively is essentially a simple dynamical behavior.
    Type of Medium: Online Resource
    ISSN: 2073-8994
    URL: Article
    DOI: 10.3390/sym10080315
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2518382-5
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    Characterization and Pathogenicity of the Porcine Deltacoronavirus Isolated in Southwest China
    MDPI AG ; 2019
    In:  Viruses Vol. 11, No. 11 ( 2019-11-18), p. 1074-
    Details
    In: Viruses, MDPI AG, Vol. 11, No. 11 ( 2019-11-18), p. 1074-
    Abstract: Porcine deltacoronavirus (PDCoV) is a newly emerging enteric pathogen in swine that causes diarrhea in neonatal piglets and creates an additional economic burden on porcine industries in Asia and North America. In this study, a PDCoV isolate, CHN-SC2015, was isolated from Sichuan Province in southwest China. The isolate was characterized by a cytopathic effect, immunofluorescence, and electron microscopy. CHN-SC2015 titers in LLC-PK cells ranged from 104.31 to 108.22 TCID50/mL during the first 30 passages. During serial passage, 11 nucleotide mutations occurred in the S gene, resulting in nine amino acid changes. A whole genome sequencing analysis demonstrated that CHN-SC2015 shares 97.5%–99.1% identity with 59 reference strains in GenBank. Furthermore, CHN-SC2015 contained 6-nt deletion and 9-nt insertion in the ORF1ab gene, 3-nt deletion in the S gene and 11-nt deletion in its 3′UTR compared with other reference strains available in GenBank. A phylogenetic analysis showed that CHN-SC2015 is more closely related to other PDCoV strains in China than to the strains from Southeast Asia, USA, Japan, and South Korea, indicating the diversity of genetic relationships and regional and epidemic characteristics among these strains. A recombination analysis indicated that CHN-SC2015 experienced recombination events between SHJS/SL/2016 and TT-1115. In vivo infection demonstrated that CHN-SC2015 is highly pathogenic to sucking piglets, causing diarrhea, vomiting, dehydration, and death. Virus was shed daily in the feces of infected piglets and upon necropsy, was found distributed in the gastrointestinal tract and in multiple organs. CHN-SC2015 is the first systematically characterized strain from southwest China hitherto reported. Our results enrich the body of information on the epidemiology, pathogenicity and molecular evolution associated with PDCoV.
    Type of Medium: Online Resource
    ISSN: 1999-4915
    URL: Article
    DOI: 10.3390/v11111074
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2516098-9
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    Deep Learning for Fully Automatic Tumor Segmentation on Serially Acquired Dynamic Contrast-Enhanced MRI Images of Triple-Negative Breast Cancer
    MDPI AG ; 2023
    In:  Cancers Vol. 15, No. 19 ( 2023-10-02), p. 4829-
    Details
    In: Cancers, MDPI AG, Vol. 15, No. 19 ( 2023-10-02), p. 4829-
    Abstract: Accurate tumor segmentation is required for quantitative image analyses, which are increasingly used for evaluation of tumors. We developed a fully automated and high-performance segmentation model of triple-negative breast cancer using a self-configurable deep learning framework and a large set of dynamic contrast-enhanced MRI images acquired serially over the patients’ treatment course. Among all models, the top-performing one that was trained with the images across different time points of a treatment course yielded a Dice similarity coefficient of 93% and a sensitivity of 96% on baseline images. The top-performing model also produced accurate tumor size measurements, which is valuable for practical clinical applications.
    Type of Medium: Online Resource
    ISSN: 2072-6694
    URL: Article
    DOI: 10.3390/cancers15194829
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2527080-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 10
    Online Resource
    Online Resource
    Assessment of Response to Neoadjuvant Systemic Treatment in Triple-Negative Breast Cancer Using Functional Tumor Volumes from Longitudinal Dynamic Contrast-Enhanced MRI
    MDPI AG ; 2023
    In:  Cancers Vol. 15, No. 4 ( 2023-02-06), p. 1025-
    Details
    In: Cancers, MDPI AG, Vol. 15, No. 4 ( 2023-02-06), p. 1025-
    Abstract: Early assessment of neoadjuvant systemic therapy (NAST) response for triple-negative breast cancer (TNBC) is critical for patient care in order to avoid the unnecessary toxicity of an ineffective treatment. We assessed functional tumor volumes (FTVs) from dynamic contrast-enhanced (DCE) MRI after 2 cycles (C2) and 4 cycles (C4) of NAST as predictors of response in TNBC. A group of 100 patients with stage I-III TNBC who underwent DCE MRI at baseline, C2, and C4 were included in this study. Tumors were segmented on DCE images of 1 min and 2.5 min post-injection. FTVs were measured using the optimized percentage enhancement (PE) and signal enhancement ratio (SER) thresholds. The Mann–Whitney test was used to compare the performance of the FTVs at C2 and C4. Of the 100 patients, 49 (49%) had a pathologic complete response (pCR) and 51 (51%) had a non-pCR. The maximum area under the receiving operating characteristic curve (AUC) for predicting the treatment response was 0.84 (p 〈 0.001) for FTV at C4 followed by FTV at C2 (AUC = 0.82, p 〈 0.001). The FTV measured at baseline was not able to discriminate pCR from non-pCR. FTVs measured on DCE MRI at C2, as well as at C4, of NAST can potentially predict pCR and non-pCR in TNBC patients.
    Type of Medium: Online Resource
    ISSN: 2072-6694
    URL: Article
    DOI: 10.3390/cancers15041025
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2527080-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...