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  • 1
    In: Agronomy, MDPI AG, Vol. 13, No. 10 ( 2023-09-27), p. 2492-
    Abstract: Initial evaluations of the System of Rice Intensification in India and elsewhere focused mainly on its impacts on yield and income, and usually covered just one or two seasons. Researchers at the ICAR-Indian Institute of Rice Research have conducted a more comprehensive evaluation of SRI methods over six years (six wet and six dry seasons), comparing them with three alternatives: modified, partially mechanized SRI (MSRI) to reduce labor requirements; direct-seeded rice (DSR) as an alternative method for growing rice; and conventional transplanting of rice with flooding of fields (CTF). Grain yield with SRI methods was found to be about 50% higher than with CTF (6.35 t ha−1 vs. 4.27 t ha−1), while the MSRI yield was essentially the same (6.34 t ha−1), 16% more than with DSR (5.45 t ha−1). Water productivity with SRI methods was 5.32–6.85 kg ha-mm−1, followed by 4.14–5.72 kg ha-mm−1 for MSRI, 5.06–5.11 kg ha-mm−1 for DSR, and 3.52–4.56 kg ha-mm−1 for CTF. In comparison with CTF, SRI methods significantly enhanced soil microbial populations over time: bacteria by 12%, fungi by 8%, and actinomycetes by 20%. Biological activity in the rhizosphere was also higher as indicated by 8.5% greater dehydrogenase and 16% more FDA enzymes in soil under SRI management. Similarly, an indicator of soil organic matter, glucosidase activity, was 78% higher compared to CTF. SRI enhanced the relative abundance of beneficial microbial-feeding nematodes by 7.5% compared to CTF, while that of plant-pathogenic nematodes was 7.5% lower under SRI. Relative to conventional methods, SRI management reduced GHG emissions by 21%, while DSR reduced them by 23%, and MSRI by 13%, compared to standard rice-growing practice. Economic analysis showed both gross and net economic returns to be higher with SRI than with the other management systems evaluated. While the six-year study documented many advantages of SRI crop management, it also showed that MSRI is a promising adaptation that provides similar benefits but with lower labor requirements.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2607043-1
    SSG: 23
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  • 2
    In: Plants, MDPI AG, Vol. 12, No. 1 ( 2022-12-28), p. 144-
    Abstract: Globally, micronutrient (iron and zinc) enriched rice has been a sustainable and cost-effective solution to overcome malnutrition or hidden hunger. Understanding the genetic basis and identifying the genomic regions for grain zinc (Zn) across diverse genetic backgrounds is an important step to develop biofortified rice varieties. In this case, an RIL population (306 RILs) obtained from a cross between the high-yielding rice variety MTU1010 and the high-zinc rice variety Ranbir Basmati was utilized to pinpoint the genomic region(s) and QTL(s) responsible for grain zinc (Zn) content. A total of 2746 SNP markers spanning a genetic distance of 2445 cM were employed for quantitative trait loci (QTL) analysis, which resulted in the identification of 47 QTLs for mineral (Zn and Fe) and agronomic traits with 3.5–36.0% phenotypic variance explained (PVE) over the seasons. On Chr02, consistent QTLs for grain Zn polished (qZnPR.2.1) and Zn brown (qZnBR.2.2) were identified. On Chr09, two additional reliable QTLs for grain Zn brown (qZnBR.9.1 and qZnBR.9.2) were identified. The major-effect QTLs identified in this study were associated with few key genes related to Zn and Fe transporter activity. The genomic regions, candidate genes, and molecular markers associated with these major QTLs will be useful for genomic-assisted breeding for developing Zn-biofortified varieties.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2704341-1
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  • 3
    In: Molecules, MDPI AG, Vol. 23, No. 10 ( 2018-10-12), p. 2620-
    Abstract: This paper provides a comparative account of the essential oil chemical composition and biological activities of five Brazilian species of Baccharis (Asteraceae), namely B. microdonta, B. pauciflosculosa, B. punctulata, B. reticularioides, and B. sphenophylla. The chemical compositions of three species (B. pauciflosculosa, B. reticularioides, and B. sphenophylla) are reported for the first time. Analyses by GC/MS showed notable differences in the essential oil compositions of the five species. α-Pinene was observed in the highest concentration (24.50%) in B. reticularioides. Other major compounds included α-bisabolol (23.63%) in B. punctulata, spathulenol (24.74%) and kongol (22.22%) in B. microdonta, β-pinene (18.33%) and limonene (18.77%) in B. pauciflosculosa, and β-pinene (15.24%), limonene (14.33%), and spathulenol (13.15%) in B. sphenophylla. In vitro analyses for antimalarial, antitrypanosomal, and insecticidal activities were conducted for all of the species. B. microdonta and B. reticularioides showed good antitrypanosomal activities; B. sphenophylla showed insecticidal activities in fumigation bioassay against bed bugs; and B. pauciflosculosa, B. reticularioides, and B. sphenophylla exhibited moderate antimalarial activities. B. microdonta and B. punctulata showed cytotoxicity. The leaves and stems of all five species showed glandular trichomes and ducts as secretory structures. DNA barcoding successfully determined the main DNA sequences of the investigated species and enabled authenticating them.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2008644-1
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  • 4
    In: Journal of Fungi, MDPI AG, Vol. 7, No. 7 ( 2021-07-14), p. 561-
    Abstract: Rhizoctonia solani AG1-1A is a necrotrophic fungus that causes sheath blight disease in rice. The reliable resistant source against this phytopathogenic fungus is not available in the gene pool of rice. Better understanding of pathogen genomics and gene regulatory networks are critical to devise alternate strategies for developing resistance against this noxious pathogen. In this study, miRNA-like RNAs (milRNAs) of an Indian strain of R. solani were identified by deep sequencing of small RNAs. We identified 128 known and 22 novel milRNAs from 20,963,123 sequence reads. These milRNAs showed 1725 target genes in the fungal genome which include genes associated with growth, development, pathogenesis and virulence of R. solani. Notably, these fungal milRNAs showed their target genes in host (rice) genome also which were later verified by qRT-PCR. The host target genes are associated with auxin metabolism, hypersensitive response, defense genes, and genes related to growth and development of rice. Osa-vacuolar-sorting receptor precursor: Rhi-milR-13, Osa-KANADI1:Rhi-milR-124, Osa-isoflavone reductase: Rhi-milR-135, Osa-nuclear transcription factor Y:Rhi-milR-131, Osa-NB-ARC domain containing protein: Rhi-milR-18, and Osa-OsFBX438: Rhi-milR-142 are notable potential regulons of host target genes: fungal milRNAs that need to be investigated for better understanding of the crosstalk of RNAi pathways between R. solani and rice. The detailed expression analysis of 17 milRNAs by qRT-PCR was analysed during infection at different time points of inoculation, at different growth stages of the host, in four different genotypes of the host, and also in four different strains of fungi which revealed differential regulation of milRNAs associated with pathogenesis and virulence. This study highlights several important findings on fungal milRNAs which need to be further studied and characterized to decipher the gene expression and regulation of this economically important phytopathogen.
    Type of Medium: Online Resource
    ISSN: 2309-608X
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2784229-0
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