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  • MDPI AG  (14)
  • 1
    Online-Ressource
    Online-Ressource
    Germacrane Sesquiterpene Dilactones from Mikania micrantha and Their Antibacterial and Cytotoxic Activity
    MDPI AG ; 2023
    In:  Molecules Vol. 28, No. 5 ( 2023-02-24), p. 2119-
    Details
    In: Molecules, MDPI AG, Vol. 28, No. 5 ( 2023-02-24), p. 2119-
    Kurzfassung: Four new germacrane sesquiterpene dilactones, 2β-hydroxyl-11β,13-dihydrodeoxymikanolide (1), 3β-hydroxyl-11β,13-dihydrodeoxymikanolide (2), 1α,3β-dihydroxy-4,9-germacradiene-12,8:15,6-diolide (3), and (11β,13-dihydrodeoxymikanolide-13-yl)-adenine (4), together with five known ones (5–9) were isolated from the aerial parts of Mikania micrantha. Their structures were elucidated on the basis of extensive spectroscopic analysis. Compound 4 is featured with an adenine moiety in the molecule, which is the first nitrogen-containing sesquiterpenoid so far isolated from this plant species. These compounds were evaluated for their in vitro antibacterial activity against four Gram-(+) bacteria of Staphyloccocus aureus (SA), methicillin-resistant Staphylococcus aureus (MRSA), Bacillus cereus (BC) and Curtobacterium. flaccumfaciens (CF), and three Gram-(–) bacteria of Escherichia coli (EC), Salmonella. typhimurium (SA), and Pseudomonas Solanacearum (PS). Compounds 4 and 7–9 were found to show strong in vitro antibacterial activity toward all the tested bacteria with the MIC values ranging from 1.56 to 12.5 µg/mL. Notably, compounds 4 and 9 showed significant antibacterial activity against the drug-resistant bacterium of MRSA with MIC value 6.25 µg/mL, which was close to reference compound vancomycin (MIC 3.125 µg/mL). Compounds 4 and 7–9 were further revealed to show in vitro cytotoxic activity toward human tumor A549, HepG2, MCF-7, and HeLa cell lines, with IC50 values ranging from 8.97 to 27.39 μM. No antibacterial and cytotoxic activity were displayed for the other compounds. The present research provided new data to support that M. micrantha is rich in structurally diverse bioactive compounds worthy of further development for pharmaceutical applications and for crop protection in agricultural fields.
    Materialart: Online-Ressource
    ISSN: 1420-3049
    URL: Article
    DOI: 10.3390/molecules28052119
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2023
    ZDB Id: 2008644-1
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Online-Ressource
    Online-Ressource
    Microrna-130a Downregulates HCV Replication through an atg5-Dependent Autophagy Pathway
    MDPI AG ; 2019
    In:  Cells Vol. 8, No. 4 ( 2019-04-10), p. 338-
    Details
    In: Cells, MDPI AG, Vol. 8, No. 4 ( 2019-04-10), p. 338-
    Kurzfassung: We previously identified that miR-130a downregulates HCV replication through two independent pathways: restoration of host immune responses and regulation of pyruvate metabolism. In this study, we further sought to explore host antiviral target genes regulated by miR-130a. We performed a RT² Profiler™ PCR array to identify the host antiviral genes regulated by miR-130a. The putative binding sites between miR-130a and its downregulated genes were predicted by miRanda. miR-130a and predicted target genes were over-expressed or knocked down by siRNA or CRISPR/Cas9 gRNA. Selected gene mRNAs and their proteins, together with HCV replication in JFH1 HCV-infected Huh7.5.1 cells were monitored by qRT-PCR and Western blot. We identified 32 genes that were significantly differentially expressed more than 1.5-fold following miR-130a overexpression, 28 of which were upregulated and 4 downregulated. We found that ATG5, a target gene for miR-130a, significantly upregulated HCV replication and downregulated interferon stimulated gene expression. miR-130a downregulated ATG5 expression and its conjugation complex with ATG12. ATG5 and ATG5-ATG12 complex affected interferon stimulated gene (ISG) such as MX1 and OAS3 expression and subsequently HCV replication. We concluded that miR-130a regulates host antiviral response and HCV replication through targeting ATG5 via the ATG5-dependent autophagy pathway.
    Materialart: Online-Ressource
    ISSN: 2073-4409
    URL: Article
    DOI: 10.3390/cells8040338
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2019
    ZDB Id: 2661518-6
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Online-Ressource
    Online-Ressource
    Influence of Multi-Step Heating Methods on Properties of Al–Si Coating Boron Steel Sheet
    MDPI AG ; 2021
    In:  Coatings Vol. 11, No. 2 ( 2021-01-30), p. 164-
    Details
    In: Coatings, MDPI AG, Vol. 11, No. 2 ( 2021-01-30), p. 164-
    Kurzfassung: In this study, the influence of the multi-step heating methods, such as two-step heating methods and three-step heating methods, on the properties of Al–Si coating boron steel sheet were evaluated by using the Gleeble-3500 thermal simulator. The evolution of microstructure and 3D surface topography of the Al–Si coating were also investigated. The results showed that the heating rates of 50 °C/s, named rapid heating, at the stage of 20–500 °C did not significantly influence the microstructure and 3D surface topography of the Al–Si coating in the two-step heating methods. The results also indicated that the volume fractions of Fe3Al2Si3 intermetallic compound, FeAl intermetallic compound and a-Fe phase in the Al–Si coating reduced by rapid heating at the stage of 700–930 °C in the three-step heating methods. The roughness of 3D surface topography of the Al–Si coating increased by rapid heating at the stage of 700–930 °C. Rapid heating at the stage of 700–930 °C had little influence on the porosity of the Al–Si coating. The results provided a theoretical basis for the popularization and application of rapid heating in the Al–Si coating boron steel sheet.
    Materialart: Online-Ressource
    ISSN: 2079-6412
    URL: Article
    DOI: 10.3390/coatings11020164
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2021
    ZDB Id: 2662314-6
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
    Online-Ressource
    Online-Ressource
    Thin-Film Lithium Cobalt Oxide for Lithium-Ion Batteries
    MDPI AG ; 2022
    In:  Energies Vol. 15, No. 23 ( 2022-11-28), p. 8980-
    Details
    In: Energies, MDPI AG, Vol. 15, No. 23 ( 2022-11-28), p. 8980-
    Kurzfassung: Lithium cobalt oxide (LCO) cathode has been widely applied in 3C products (computer, communication, and consumer), and LCO films are currently the most promising cathode materials for thin-film lithium batteries (TFBs) due to their high volumetric energy density and favorable durability. Most LCO thin films are fabricated by physical vapor deposition (PVD) techniques, while the influence of preparation on the materials’ properties and electrochemical performance has not been highlighted. In this review, the dominant effects (heating, substrate, power, atmosphere, etc.) on LCO thin films are summarized, and the LCO thin films fabricated by other techniques (spin coating, sol–gel, atomic layer deposition, pulsed laser deposition, etc.) are outlined. Moreover, the modification strategies including bulk doping and surface coating for powder and thin-film LCO electrodes are discussed in detail. This review may pave the way for developing novel, durable, and high-performance LCO thin films by versatile methods for TFB and other energy storage devices.
    Materialart: Online-Ressource
    ISSN: 1996-1073
    URL: Article
    DOI: 10.3390/en15238980
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2022
    ZDB Id: 2437446-5
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 5
    Online-Ressource
    Online-Ressource
    Water Use of Fossil Energy Production and Supply in China
    MDPI AG ; 2017
    In:  Water Vol. 9, No. 7 ( 2017-07-12), p. 513-
    Details
    In: Water, MDPI AG, Vol. 9, No. 7 ( 2017-07-12), p. 513-
    Materialart: Online-Ressource
    ISSN: 2073-4441
    URL: Article
    DOI: 10.3390/w9070513
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2017
    ZDB Id: 2521238-2
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 6
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    Optimal Obesity- and Lipid-Related Indices for Predicting Metabolic Syndrome in Chronic Kidney Disease Patients with and without Type 2 Diabetes Mellitus in China
    MDPI AG ; 2022
    In:  Nutrients Vol. 14, No. 7 ( 2022-03-23), p. 1334-
    Details
    In: Nutrients, MDPI AG, Vol. 14, No. 7 ( 2022-03-23), p. 1334-
    Kurzfassung: Existing obesity- and lipid-related indices are inconsistent with metabolic syndrome (MetS) in chronic kidney disease (CKD) patients. We compared seven indicators, including waist circumference (WC), body mass index (BMI), visceral fat area (VFA), subcutaneous fat area (SFA), visceral adiposity index (VAI), Chinese VAI and lipid accumulation product (LAP), to evaluate their ability to predict MetS in CKD patients with and without Type 2 diabetes mellitus (T2DM) under various criteria. Multivariate logistic regression analysis was used to investigate the independent associations between the indices and metabolic syndrome among 547 non-dialysis CKD patients, aged ≥18 years. The predictive power of these indices was assessed using receiver operating characteristic (ROC) curve analysis. After adjusting for potential confounders, the correlation between VAI and MetS was strongest based on the optimal cut-off value of 1.51 (sensitivity 86.84%, specificity 91.18%) and 2.35 (sensitivity 83.54%, specificity 86.08%), with OR values of 40.585 (8.683–189.695) and 5.076 (1.247–20.657) for males and females with CKD and T2DM. In CKD patients without T2DM, based on the optimal cut-off values of 1.806 (sensitivity 98.11%, specificity 72.73%) and 3.11 (sensitivity 84.62%, specificity 83.82%), the OR values were 7.514 (3.757–15.027) and 3.008 (1.789–5.056) for males and females, respectively. The area under ROC curve (AUC) and Youden index of VAI were the highest among the seven indexes, indicating its superiority in predicting MetS in both male and female CKD patients, especially those with T2DM.
    Materialart: Online-Ressource
    ISSN: 2072-6643
    URL: Article
    DOI: 10.3390/nu14071334
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2022
    ZDB Id: 2518386-2
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 7
    Online-Ressource
    Online-Ressource
    Th17/Regulatory T-Cell Imbalance and Acute Kidney Injury in Patients with Sepsis
    MDPI AG ; 2022
    In:  Journal of Clinical Medicine Vol. 11, No. 14 ( 2022-07-12), p. 4027-
    Details
    In: Journal of Clinical Medicine, MDPI AG, Vol. 11, No. 14 ( 2022-07-12), p. 4027-
    Kurzfassung: To analyze the predictive value of the Th17/Treg ratio for renal injury in sepsis patients, a prospective observational study was conducted. Adult patients with sepsis were enrolled and divided into a sepsis-induced acute kidney injury (SAKI) group and a sepsis-without-AKI group. Logistic regression was used to analyze the independent predictors of SAKI, and the ROC curve was plotted to evaluate the predictive value of the Th17/Treg ratio for renal injury in patients with sepsis. A total of 124 patients were enrolled in this study, including 60 cases (48.39%) of SAKI. Patients who developed sepsis-induced acute kidney injury had a higher Th17/Treg ratio level compared to patients without it (0.11 [0.07, 0.28] versus 0.06 [0.05, 0.16] , p 〈 0.05, respectively. The area under the receiver operating characteristic curve of the Th17/Treg ratio to predict sepsis-induced acute kidney injury was 0.669 (95% CI 0.574–0.763, p 〈 0.05). The Th17/Treg ratio was associated with SAKI (OR 1.15, 95%CI [1.06–1.24], p 〈 0.05, non-adjusted and R 1.12, 95%CI [1.00–1.25], p 〈 0.05, adjusted). The use of the Th17/Treg ratio improved the prediction performance of the prediction model of NAGL. The median Th17/Treg ratio significantly increased with the stratified KDIGO stage (p 〈 0.05). Th17/Treg imbalance was associated with occurrence of acute kidney injury and AKI severity in patients with sepsis. The Th17/Treg ratio could be a potential predictive marker of sepsis-induced acute kidney injury.
    Materialart: Online-Ressource
    ISSN: 2077-0383
    URL: Article
    DOI: 10.3390/jcm11144027
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2022
    ZDB Id: 2662592-1
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 8
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    Computational Docking as a Tool in Guiding the Drug Design of Rutaecarpine Derivatives as Potential SARS-CoV-2 Inhibitors
    MDPI AG ; 2024
    In:  Molecules Vol. 29, No. 11 ( 2024-06-03), p. 2636-
    Details
    In: Molecules, MDPI AG, Vol. 29, No. 11 ( 2024-06-03), p. 2636-
    Kurzfassung: COVID-19 continues to spread around the world. This is mainly because new variants of the SARS-CoV-2 virus emerge due to genomic mutations, evade the immune system and result in the effectiveness of current therapeutics being reduced. We previously established a series of detection platforms, comprising computational docking analysis, S-protein-based ELISA, pseudovirus entry, and 3CL protease activity assays, which allow us to screen a large library of phytochemicals from natural products and to determine their potential in blocking the entry of SARS-CoV-2. In this new screen, rutaecarpine (an alkaloid from Evodia rutaecarpa) was identified as exhibiting anti-SARS-CoV-2 activity. Therefore, we conducted multiple rounds of structure-activity-relationship (SAR) studies around this phytochemical and generated several rutaecarpine analogs that were subjected to in vitro evaluations. Among these derivatives, RU-75 and RU-184 displayed remarkable inhibitory activity when tested in the 3CL protease assay, S-protein-based ELISA, and pseudovirus entry assay (for both wild-type and omicron variants), and they attenuated the inflammatory response induced by SARS-CoV-2. Interestingly, RU-75 and RU-184 both appeared to be more potent than rutaecarpine itself, and this suggests that they might be considered as lead candidates for future pharmacological elaboration.
    Materialart: Online-Ressource
    ISSN: 1420-3049
    URL: Article
    DOI: 10.3390/molecules29112636
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2024
    ZDB Id: 2008644-1
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 9
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    An Exploratory Study on a New Method for Nutritional Status Assessment in Patients with Chronic Kidney Disease
    MDPI AG ; 2023
    In:  Nutrients Vol. 15, No. 11 ( 2023-06-05), p. 2640-
    Details
    In: Nutrients, MDPI AG, Vol. 15, No. 11 ( 2023-06-05), p. 2640-
    Kurzfassung: Malnutrition is a risk factor for disease progression and poor prognosis in chronic kidney disease (CKD). However, the complexity of nutritional status assessment limits its clinical application. This study explored a new method of nutritional assessment in CKD (stage 1–5) patients using the Subjective Global Assessment (SGA) as the gold standard and evaluated its applicability. The kappa test was used to analyze the consistency of the Renal Inpatient Nutrition Screening Tool (Renal iNUT) with SGA and protein-energy wasting. Logistic regression analysis was used to analyze the risk factors of CKD malnutrition and calculate the prediction probability of multiple indicators combined for the diagnosis of CKD malnutrition. The receiver operating characteristic curve of the prediction probability was drawn to evaluate its diagnostic efficiency. A total of 161 CKD patients were included in this study. The prevalence of malnutrition according to SGA was 19.9%. The results showed that Renal iNUT had a moderate consistency with SGA and a general consistency with protein-energy wasting. Age 〉 60 years (odds ratio, OR = 6.78), neutrophil–lymphocyte ratio 〉 2.62 (OR = 3.862), transferrin 〈 200 mg/dL (OR = 4.222), phase angle 〈 4.5° (OR = 7.478), and body fat percentage 〈 10% (OR = 19.119) were risk factors for malnutrition in patients with CKD. The area under the receiver operating characteristic curve of multiple indicators for the diagnosis of CKD malnutrition was 0.89 (95% confidence interval: 0.834–0.946, p 〈 0.001). This study demonstrated that Renal iNUT has good specificity as a new tool for the nutrition screening of CKD patients, but its sensitivity needs to be optimized. Advanced age, high neutrophil–lymphocyte ratio, low transferrin level, low phase angle, and low body fat percentage are risk factors for malnutrition in patients with CKD. The combination of the above indicators has high diagnostic efficiency in the diagnosis of CKD malnutrition, which may be an objective, simple, and reliable method to evaluate the nutritional status of patients with CKD.
    Materialart: Online-Ressource
    ISSN: 2072-6643
    URL: Article
    DOI: 10.3390/nu15112640
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2023
    ZDB Id: 2518386-2
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 10
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    A New and Practical Synthetic Method for the Synthesis of 6-O-Methyl-scutellarein: One Metabolite of Scutellarin in Vivo
    MDPI AG ; 2015
    In:  International Journal of Molecular Sciences Vol. 16, No. 4 ( 2015-04-03), p. 7587-7594
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 16, No. 4 ( 2015-04-03), p. 7587-7594
    Kurzfassung: Scutellarin (1) has been used for the treatment of angina pectoris, cerebral infarction and coronary heart disease with a large market share in China. Pharmacokinetic studies on scutellarin showed that scutellarin (1) is readily converted into its metabolites in vivo. In this paper, a new and practical synthetic method for the synthesis of 6-O-methyl-scutellarein (3) (one metabolite of scutellarin in vivo) is reported. The benzyl bromide was firstly used to selectively replace the acetyl group at C-7 in 7, and was then used to protect the hydroxy groups at C-4' in 10, 6-O-methyl-scutellarein (3) is obtained in high yield through these methods.
    Materialart: Online-Ressource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms16047587
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2015
    ZDB Id: 2019364-6
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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