Publication Date:
2022-05-25
Description:
© The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in MicrobiologyOpen 7 (2018): e00586, doi:10.1002/mbo3.586.
Description:
Chemoautotrophic bacteria belonging to the genus Sulfurimonas in the class
Campylobacteria are widespread in many marine environments characterized by redox
interfaces, yet little is known about their physiological adaptations to different environmental
conditions. Here, we used liquid chromatography coupled with tandem
mass spectrometry (LC-MS/
MS) in a targeted metabolomics approach to study the
adaptations of Sulfurimonas denitrificans to varying salt concentrations that are found
in its natural habitat of tidal mudflats. Proline was identified as one of the most abundant
internal metabolites and its concentration showed a strong positive correlation
with ionic strength, suggesting that it acts as an important osmolyte in S. denitrificans.
2,3-dihydroxypropane-
1-
sulfonate
was also positively correlated with ionic strength,
indicating it might play a previously unrecognized role in osmoregulation. Furthermore,
the detection of metabolites from the reductive tricarboxylic acid cycle at high internal
concentrations reinforces the importance of this pathway for carbon fixation in
Campylobacteria and as a hub for biosynthesis. As the first report of metabolomic data
for an campylobacterial chemolithoautotroph, this study provides data that will be
useful to understand the adaptations of Campylobacteria to their natural habitat at
redox interfaces.
Description:
NSF, Grant/Award Number: OCE-
1136727 and OCE-1154320; Woods Hole
Oceanographic Institution; U.S. Geological
Survey
Keywords:
Environmental stress
;
Metabolism
;
Metabolomics
;
Microbial ecology
;
Osmoregulation
;
Sulfurimonas
Repository Name:
Woods Hole Open Access Server
Type:
Article
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