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  • 1
    Online Resource
    Online Resource
    Zoo Workers. (2015)
    New York, NY :Rosen Publishing Group,
    Details
    Keywords: Electronic books.
    Description / Table of Contents: Working at a zoo is a great career path for someone who loves animals and isn't afraid of hands-on work. Through engaging text and fun fact boxes, readers discover what zoo workers do and how a person can prepare for a career in this field. Additional information is provided in a clear graphic organizer. Colorful photographs of zoo animals and the workers who care for them keep readers entertained with each turn of the page. It takes special skills to be a successful zoo worker, and readers discover what those skills are as they learn about this exciting career.
    Type of Medium: Online Resource
    Pages: 1 online resource (26 pages)
    Edition: 1st ed.
    ISBN: 9781508143789
    Series Statement: Hands-On Jobs Series
    URL: https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=5141242
    DDC: 590.73
    Language: English
    Note: Cover -- Title Page -- Copyright -- Contents -- A Wild Job! -- Doing the Dirty Work -- New Experiences -- Helpful Training -- Special Skills and Qualities -- Strong and Safe -- Studying Science -- Hands-On Experience -- Other Zoo Workers -- Worth the Hard Work -- Glossary -- Index -- Websites -- Back Cover.
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  • 2
    Electronic Resource
    Electronic Resource
    Crystallization and preliminary X-ray diffraction data of the complex of recombinant tick anticoagulant peptide (rTAP) and bovine factor Xa (1999)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 55 (1999), S. 862-864 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The complex of bovine factor Xa and recombinant tick anticoagulant peptide (rTAP) was crystallized in two different crystal forms using polyethylene glycol as a precipitant. Form I belongs to space group P42212 with unit-cell dimensions a = b = 133.1, c = 68.8 Å. It contains one complex per asymmetric unit and diffracts to 3.0 Å resolution. Form II belongs to P41212 (or P43212) with dimensions a = b = 126.5, c = 146.7 Å; it contains two complexes per asymmetric unit and diffracts to 2.5 Å. The crystals of both forms consist of factor Xa (MW = 45.3 kDa) and rTAP (MW = 6.7 kDa).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444998017673
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  • 3
    Electronic Resource
    Electronic Resource
    Purification of Total RNA from Human Stool Samples (1998)
    Springer
    Digestive diseases and sciences 43 (1998), S. 2652-2658 
    Details
    ISSN: 1573-2568
    Keywords: RNA ; STOOL ; REVERSE TRANSCRIPTION-POLYMERASE CHAINREACTION ; GENE EXPRESSION ; COLON CANCER
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract While colonoscopy may detect early-stage colontumors, a less invasive and more costeffective techniquewould be beneficial. Stool, which picks up sloughed-offcolonic epithelial cells, would be ideal for sampling the mucosa; shed tumor cells maydisplay alterations in gene expression observed inintact tumors. It is first necessary, however, to showthat RNA can be isolated from human feces and that this RNA contains human gene transcripts. We havetherefore developed a method for the isolation of totalRNA from freshly passed human stool, consisting of lysisin chaotropic agents, repeated extraction with phenol and phenolchloroform, and absorptionwith an RNA-binding resin. After treatment withRNase-free DNase I, we assayed these preparations forthe presence of human RNA by quantitative slot blotting, northern blotting, and reversetranscription-polymerase chain reaction (RT-PCR). Weobtained 5-30 μg RNA per gram of stool from cancerpatients, and about 5 μg RNA per gram of controlstool. Quantitative slot blotting showed that about 10% of this RNAwas of human origin. Both northern blotting and RT-PCRdemonstrated the presence of human RNA in these samples.To unambiguously demonstrate the isolation of RNA from stool, we incubated a mixture ofrat cells and control human stool at 37°C for up to24 hr. RT-PCR of the RNA isolated from this sampleclearly revealed the presence of rat-specific mRNA.These experiments indicate that RNA can be isolatedfrom human stool and that message encoded by human genescan be assayed in these preparations. This procedure mayprovide a powerful tool to identify patients at risk for colon cancer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026699126899
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of protooncogene-encoded mRNA by colonic epithelial cells in inflammatory bowel disease (1996)
    Springer
    Digestive diseases and sciences 41 (1996), S. 660-669 
    Details
    ISSN: 1573-2568
    Keywords: inflammatory bowel disease ; ulcerative colitis ; protooncogenes ; gene expression ; c-fos ; c-abl ; c-yes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Protooncogenes are cell cycle-related genes that are involved in cell growth or proliferation. Alterations in the level of expression of these genes, or expression of aberrant gene products, have been observed in tumors and precancerous conditions. To determine if expression of these genes is altered in patients with inflammatory bowel disease (IBD)-who are at risk for development of colon cancer—we assayed transcripts of 15 protooncogenes in colonic epithelial cells of IBD patients and controls. Nine, of these genes (H-ras, c-myc, c-fos, c-jun, junB, N-myc, c-abl, c-yes, andp53) were expressed in epithelial cells, whereas two (RB1 and N-ras) were not. Expression of four other genes (c-src, K-ras, c-raf and c-myb) was observed, but the intensity of these bands was too low for densitometric analysis. The steady-state levels of transcripts of H-ras and five nuclear protooncogenes (c-myc, c-fos, c-jun, junB, and N-myc) were lower in epithelial cells from involved or uninvolved IBD samples than in normal epithelial cells from either sporadic colon cancer or diverticulitis patients. The level of c-fos mRNA was two-to threefold higher in involved than in uninvolved areas of the colons of two ulcerative colitis (UC) patients, but not in one Crohn's disease (CD) patient. Message abundance of c-abl transcripts was two-to threefold lower in UC epithelial cells than in either the CD or control samples. The steady-state level of c-yes-encoded mRNA was considerably higher in IBD patients resected for colon cancer than in patients resected for active chronic IBD or in controls. The level ofp53 message was constant in these samples. Increased levels of c-fos mRNA in involved UC relative to uninvolved, UC may be related to the disease process. Decreased expression of c-abl transcripts in UC may be a diagnostic marker for UC and may be related to the rate of cell turnover in these diseases. Enhanced expression of c-yes in IBD patients with tumors compared to active chronic IBD and controls suggests that expression of this gene may be a marker for development of colon cancer in IBD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02213120
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Expression of growth factor receptor-encoded mRNA by colonic epithelial cells is altered in inflammatory bowel diseas (1995)
    Springer
    Digestive diseases and sciences 40 (1995), S. 485-494 
    Details
    ISSN: 1573-2568
    Keywords: IBD ; ulcerative colitis ; growth factors ; growth factor receptors ; gene expression ; PDGF-R-β
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A link between inflammation of the colon in inflammatory bowel disease (IBD) and the increased risk of colon cancer in ulcerative colitis (UC) may be provided by growth factor receptor genes. Their expression may be altered in response to growth factors present in the mucosa, and this, in turn, may induce further genetic changes, linked to carcinogenesis, in the cells of the colonic epithelium. To test this hypothesis, we assayed steady-state levels of eight growth factor receptor mRNAs in colonic epithelial cells of IBD patients and controls. Four of these genes (EGF-R, IGFI-R, CSF1-R, andPDGF-R-β) were expressed in epithelial cells, whereas four (erbB-2, erbB-3, NGF-R, andmet) were not. The level of the former in involved or uninvolved IBD was considerably lower than in normal epithelial cells from either sporadic colon cancer or diverticulitis patients. In contrast, expression was much higher in IBD patients with colon tumors than in active chronic IBD. The level of PDGF-R-β mRNA was two- to fourfold higher in involved than in uninvolved areas of the colons of two UC patients, but not in one Crohn's disease patient. Message abundance of its ligand,PDGF-β, however, was the same in paired UC samples. The pattern of expression ofPDGF-β andcripto was identical to that ofEGF-R, whereas the level of mRNA of amphiregulin was the same in active chronic IBD and IBD patients with tumors. A fourth growth factor,Kfgf, was not expressed. Increased levels of PDGF-R-β mRNA in involved UC relative to uninvolved UC may be related to the disease process in UC. Decreased expression of growth factor- and growth factor receptor-encoded mRNA in active chronic IBD may be related to the disease process, or it may be an effect of steroid therapy undergone by these patients. Enhanced expression of these genes in IBD patients with tumors compared to those without tumors suggests that this may be a marker for development of colon cancer in IBD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02064355
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  • 6
    Electronic Resource
    Electronic Resource
    Culture theory: The developing synthesis from biology (1982)
    Springer
    Human ecology 10 (1982), S. 383-400 
    Details
    ISSN: 1572-9915
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Conclusions We believe that a useful, complete theory of culture is simpler than the dichotomies promoted by the coevolutionary approach suggest. Culture can be regarded as an aspect of the environment into which each human is born and must succeed or fail, developed gradually by the succession of humans who have lived throughout history. We hypothesize that culture results from the inclusive-fitness-maximizing efforts of all humans who have lived. We think the evidence suggests that cultural traits are, in general, vehicles of genic survival, and that the heritability of cultural traits depends on the judgments (conscious and unconscious) of individuals with regard to their effects on the individual's inclusive fitness. The challenge now before students of culture is to understand the proximate mechanisms, especially the ontogeny of learning biases, that result in the acquisition and transmission of cultural traits. How, for example, do we learn what constitutes an appropriate return on a social investment in different circumstances; i.e., what causes us to feel rewarded by, say, helping offspring who do not help us back, yet consistently to begrudge lesser expenditures to most others, or to feel cheated if we are not compensated for such expenditures immediately? We suggest that the answers to such questions lie in a few basic evolved learning tendencies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01531192
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Streamflow-induced variations in nitrate flux in tributaries to the Atlantic Coastal Zone (1996)
    Springer
    Biogeochemistry 33 (1996), S. 149-177 
    Details
    ISSN: 1573-515X
    Keywords: Atlantic estuaries ; climate change ; climatic variability ; coastal management ; nitrate flux ; seasonal variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Streamflow-related variability in nutrient flux represents an important source of uncertainty in managing nutrient inputs to coastal ecosystems. Quantification of flux variability is of particular interest to coastal resource managers in adopting effective nutrient-reduction goals and monitoring progress towards these goals. We used historical records of streamflow and water-quality measurements for 104 river monitoring stations in an analysis of variability in annual and seasonal flux of nitrate to the Atlantic coastal zone. We present two measures of temporal flux variability: the coefficient of variation (CV) and the exceedence probability (EP) of 1.5 times the median flux. The magnitude of flux variations spans a very wide range and depends importantly upon the season of year and the climatic and land-use characteristics of the tributary watersheds. Year-to-year variations (CV) in annual mean flux range over two orders of magnitude, from 3–200% of the long-term mean flux, although variations more typically range from 20–40% of the long-term mean. The annual probability of exceeding the long-term median flux by more than 50% (EP) is less than 0.10 in most rivers, but is between 0.10 and 0.35 in 40% of the rivers. Year-to-year variability in seasonal mean flux commonly exceeds that in annual flux by a factor of 1.5 to 4. In western Gulf of Mexico coastal rivers, the year-to-year variablity in the seasonal mean flux is larger than in other regions, and is of a similar magnitude in all seasons. By contrast, in Atlantic coastal rivers, the winter and spring seasons, which account for about 70% of the annual flux, display the smallest relative variability in seasonal mean flux. We quantify the elasticity of nutrient flux to hypothetical changes in Streamflow (i.e., the percent increase in flux per percentage increase in mean discharge) to allow the approximation of flux variability from streamflow records and the estimation of the effects of future climatically-induced changes in Streamflow on nutrient flux. Flux elasticities are less than unity (median = 0.93%) at most stations, but vary widely from 0.05% to 1.59%. Elasticities above unity occur most frequently in the largest rivers and in rivers draining the arid portions of the western Gulf of Mexico Basin. Historical flux variability and elasticity generally increase with the extent of arid conditions and the quantity of nonurban land use in the watershed. We extend the analysis of flux variability to examine several case studies of highly unusual meteorological events capable of significantly elevating nitrate flux and degrading estuarine ecology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181070
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