ISSN:
1573-2568
Keywords:
IBD
;
ulcerative colitis
;
growth factors
;
growth factor receptors
;
gene expression
;
PDGF-R-β
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract A link between inflammation of the colon in inflammatory bowel disease (IBD) and the increased risk of colon cancer in ulcerative colitis (UC) may be provided by growth factor receptor genes. Their expression may be altered in response to growth factors present in the mucosa, and this, in turn, may induce further genetic changes, linked to carcinogenesis, in the cells of the colonic epithelium. To test this hypothesis, we assayed steady-state levels of eight growth factor receptor mRNAs in colonic epithelial cells of IBD patients and controls. Four of these genes (EGF-R, IGFI-R, CSF1-R, andPDGF-R-β) were expressed in epithelial cells, whereas four (erbB-2, erbB-3, NGF-R, andmet) were not. The level of the former in involved or uninvolved IBD was considerably lower than in normal epithelial cells from either sporadic colon cancer or diverticulitis patients. In contrast, expression was much higher in IBD patients with colon tumors than in active chronic IBD. The level of PDGF-R-β mRNA was two- to fourfold higher in involved than in uninvolved areas of the colons of two UC patients, but not in one Crohn's disease patient. Message abundance of its ligand,PDGF-β, however, was the same in paired UC samples. The pattern of expression ofPDGF-β andcripto was identical to that ofEGF-R, whereas the level of mRNA of amphiregulin was the same in active chronic IBD and IBD patients with tumors. A fourth growth factor,Kfgf, was not expressed. Increased levels of PDGF-R-β mRNA in involved UC relative to uninvolved UC may be related to the disease process in UC. Decreased expression of growth factor- and growth factor receptor-encoded mRNA in active chronic IBD may be related to the disease process, or it may be an effect of steroid therapy undergone by these patients. Enhanced expression of these genes in IBD patients with tumors compared to those without tumors suggests that this may be a marker for development of colon cancer in IBD.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02064355
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