In:
Сибирский научный медицинский журнал, Institute of Cytology and Genetics, SB RAS, Vol. 43, No. 6 ( 2024-01-11), p. 117-124
Abstract:
The aim of the work is to study the effect of new azoloazine derivatives on the level of DNA damage to Vero cells (DNA comet test) in vitro by alkaline gel electrophoresis. Material and methods . The objects of the study are 8-(piperidinocarbonyl)3-cyclohexylimidazo[5,1-d][1,2,3,5] tetrazine-4(3H)-one (1), diethyl ether 4-aminoimidazo[5,1-c][1,2,4] triazine3,8-dicarboxylic acid (2), 4-amino-8-ethoxycarbonylimidazo[5,1-c][1,2,4] triazine-3-N-(p-toluyl)carboxamide (3). Epirubicin was chosen as a comparison drug. The compounds were used in doses of 1/2, 1/10 and 1/50 IC50. The Vero cell line cultured according to the standard protocol was selected as the cell model. To assess genotoxicity, an alkaline version of the DNA comet method was used, which has a high sensitivity and allows detecting DNA damage. Results. Analysis of the data obtained indicates that the tested compounds 1-3 enhance DNA damage in non-tumor cells. Compound 1 has the most pronounced genotoxic effect. Thus, the use of this substance in a dose of ½ IC50 led to a significant increase in the length of the comet’s tail by 1.5 times. It was noted that DNA damage under the action of compound 1 in the studied doses and of epirubicin was on the same level. Conclusions. The results obtained prove that compounds 1-3 may have a potentially carcinogenic effect. However, this assumption requires further in-depth experimental studies.
Type of Medium:
Online Resource
ISSN:
2410-2520
,
2410-2512
DOI:
10.18699/SSMJ20230614
Language:
Unknown
Publisher:
Institute of Cytology and Genetics, SB RAS
Publication Date:
2024
Permalink
