In:
Journal of Physics D: Applied Physics, IOP Publishing, Vol. 52, No. 38 ( 2019-09-18), p. 385403-
Abstract:
It is often difficult to implement complex microscopy systems without spherical aberration. Herein, we developed a novel, robust, three-dimensional (3D), bifocal plane, single-particle tracking technique, based on a dual-objective fluorescent reflection system with spherical aberration (DOFR–SA). It can simultaneously image a pair of focused and defocused planes containing fluorescent particles with a single camera instead of splitting photons into two channels. Based on the 3D DOFR–SA, the desired position accuracy along the z -axis was achieved without compromising the precisions of the ( x , y ) positions, even with limited number of photons from a single molecule. Accordingly, this method was applied to fluorescent particle tracking in biofluids and living cells with high-spatial and temporal precisions.
Type of Medium:
Online Resource
ISSN:
0022-3727
,
1361-6463
DOI:
10.1088/1361-6463/ab2d82
Language:
Unknown
Publisher:
IOP Publishing
Publication Date:
2019
detail.hit.zdb_id:
209221-9
detail.hit.zdb_id:
1472948-9
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